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The map label for this gene is ilvD2 [H]

Identifier: 116690482

GI number: 116690482

Start: 2733165

End: 2734838

Strand: Direct

Name: ilvD2 [H]

Synonym: Bcen2424_2462

Alternate gene names: 116690482

Gene position: 2733165-2734838 (Clockwise)

Preceding gene: 116690479

Following gene: 116690483

Centisome position: 78.45

GC content: 66.61

Gene sequence:

>1674_bases
ATGTCGTACAACCGCCGCTCTAAAAACATCACGCAAGGCGTGGCCCGTTCGCCAAACCGGTCGATGTATTACGCCCTCGG
CTACCAGAAGGACGATTTCGACAAGCCGATGGTCGGCGTCGCGAACGGCCACTCGACGATCACGCCGTGCAATTCCGGCC
TGCAGCGCCTGTCGGACGCGGCCGTGGATGCCGTGAAGGCGGCCGGCGCGAACCCGCAGATCTTCGGCACGCCGACGATT
TCGGACGGCATGTCGATGGGCACCGAAGGGATGAAGTACTCGCTCGTGTCGCGCGAAGTGATCGCCGACTGCATCGAGAC
CTGCGTGCAGGGCCAGTGGATGGACGGCGTGGTGGTGGTCGGCGGCTGCGACAAGAACATGCCCGGCGGCATGATCGCGC
TCGCACGCCTGAACGTGCCGGGCATCTACGTGTACGGCGGCACGATCCGCCCGGGCAACTGGAAGGGCCGCGACCTGACG
ATCGTGTCGTCGTTCGAGGCCGTCGGCGAATTCACCGCGGGCCGGATGTCGCAGGAAGACTTCGAAGGCGTCGAGAAGAA
TGCGTGCCCGACGTCGGGTTCGTGCGGCGGCATGTACACCGCGAACACGATGAGTTCGTCGTTCGAGGCGCTCGGGATGT
CGCTGCTGTATTCGTCGACGATGGCGAACCCCGACCAGGAAAAAGTCGATTCGGCCGCCGAATCGGCACGCGTGCTGGTC
GAGGCGGTGAAGCGCGACCTGAAGCCGCGCGACATCATCACGAAGGAATCCATCGAGAACGCGGTGTCGGTGATCATGGC
CACCGGCGGCTCGACCAATGCGGTGCTGCACTATCTCGCGATCGCGCACGCGGCCGAAGTCGACTGGACGATCGACGACT
TCGAGCGCATTCGCAAGCGCGTGCCGGTGATCTGCGACCTGAAGCCGTCGGGACAATACGTCGCGACCGACCTACACCGG
GCCGGCGGGATTCCGCAGGTGCTGAAGATCCTGCTCGATGCGGGGCTGCTGCACGGCGACTGCCTGACGATCACCGGCCG
CACGATCGCCGACGAGCTGAAGGACGTGCCGGCCGTGCCGCGCGCGGACCAGCATGTGATCTTCCCGATCGACCGCGCAC
TGTACAAGGAAGGCCACCTGGCGATCCTGAAGGGCAATCTCGCGGAAGACGGCGCGGTCGCGAAGATCACCGGCCTGAAG
AACCCGGTGATCACGGGCCCGGCGCGCGTGTTCGACGACGAACAGAGCGCGATGGACGCGATCCTCGGCGACCGGATTCG
CGCGGGCGACATCCTCGTGCTGCGCTACCTCGGCCCGCAGGGCGGCCCCGGCATGCCGGAAATGCTCGCGCCAACTTCCG
CGATCATCGGCAAGGGACTCGGCGAATCGGTCGGCTTCATCACGGACGGACGCTTCTCGGGCGGCACCTGGGGCATGGTG
GTCGGCCACGTCGCGCCGGAAGCGTTCGTCGGCGGCACGATCGCGCTGGTGCAGGAAGGCGATTCGATCACGATCGACGC
GCACCGGCTGCTGCTGCAATTGAACGTCGACGAGGCGGAACTCGCCCGGCGCCGCGCCGCGTGGCGACAGCCGGCGCCGC
GCTATACGCGCGGGGTGCTCGCGAAATTCGCGGTGCTCGCACGACCGGCGAACCAGGGGGCGGTCACGGGTTGA

Upstream 100 bases:

>100_bases
AATAATATATTGGACATCCGATAACTGAAACTCCATGCTTGGTTGATCCACCCGGCGCACCGGTCGCGCCGGCGTCGCCA
AAACAACGATGGAGCTCCCC

Downstream 100 bases:

>100_bases
AGCAGGGCCGCAAGGCATGCGACCCCGATAAACGAGGCCGCCGGGAACGCGCAAAACGTGCTTCCCCTTTGCTTCTATAA
TGCGCGGACCATGAAGCCGG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD 2 [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSYNRRSKNITQGVARSPNRSMYYALGYQKDDFDKPMVGVANGHSTITPCNSGLQRLSDAAVDAVKAAGANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGNWKGRDLT
IVSSFEAVGEFTAGRMSQEDFEGVEKNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKRDLKPRDIITKESIENAVSVIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHR
AGGIPQVLKILLDAGLLHGDCLTITGRTIADELKDVPAVPRADQHVIFPIDRALYKEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMDAILGDRIRAGDILVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHRLLLQLNVDEAELARRRAAWRQPAPRYTRGVLAKFAVLARPANQGAVTG

Sequences:

>Translated_557_residues
MSYNRRSKNITQGVARSPNRSMYYALGYQKDDFDKPMVGVANGHSTITPCNSGLQRLSDAAVDAVKAAGANPQIFGTPTI
SDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGNWKGRDLT
IVSSFEAVGEFTAGRMSQEDFEGVEKNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
EAVKRDLKPRDIITKESIENAVSVIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHR
AGGIPQVLKILLDAGLLHGDCLTITGRTIADELKDVPAVPRADQHVIFPIDRALYKEGHLAILKGNLAEDGAVAKITGLK
NPVITGPARVFDDEQSAMDAILGDRIRAGDILVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
VGHVAPEAFVGGTIALVQEGDSITIDAHRLLLQLNVDEAELARRRAAWRQPAPRYTRGVLAKFAVLARPANQGAVTG
>Mature_556_residues
SYNRRSKNITQGVARSPNRSMYYALGYQKDDFDKPMVGVANGHSTITPCNSGLQRLSDAAVDAVKAAGANPQIFGTPTIS
DGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVVGGCDKNMPGGMIALARLNVPGIYVYGGTIRPGNWKGRDLTI
VSSFEAVGEFTAGRMSQEDFEGVEKNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLVE
AVKRDLKPRDIITKESIENAVSVIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKRVPVICDLKPSGQYVATDLHRA
GGIPQVLKILLDAGLLHGDCLTITGRTIADELKDVPAVPRADQHVIFPIDRALYKEGHLAILKGNLAEDGAVAKITGLKN
PVITGPARVFDDEQSAMDAILGDRIRAGDILVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMVV
GHVAPEAFVGGTIALVQEGDSITIDAHRLLLQLNVDEAELARRRAAWRQPAPRYTRGVLAKFAVLARPANQGAVTG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=604, Percent_Identity=37.9139072847682, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI2367371, Length=542, Percent_Identity=32.2878228782288, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786464, Length=468, Percent_Identity=35.2564102564103, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788157, Length=470, Percent_Identity=30.8510638297872, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=44.8214285714286, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59224; Mature: 59093

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNRRSKNITQGVARSPNRSMYYALGYQKDDFDKPMVGVANGHSTITPCNSGLQRLSDA
CCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCEECCHHHHHHHHHHH
AVDAVKAAGANPQIFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARLNVPGIYVYGGTIRPGNWKGRDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHHCCCCHHH
FEGVEKNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKRDLKPRDIITKESIENAVSVIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKR
HHHHHCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VPVICDLKPSGQYVATDLHRAGGIPQVLKILLDAGLLHGDCLTITGRTIADELKDVPAVP
CCEEEECCCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCC
RADQHVIFPIDRALYKEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMDA
CCCCEEEEECHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILGDRIRAGDILVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHCCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHRLLLQLNVDEAELARRRAAWRQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHH
AKFAVLARPANQGAVTG
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYNRRSKNITQGVARSPNRSMYYALGYQKDDFDKPMVGVANGHSTITPCNSGLQRLSDA
CCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCEECCHHHHHHHHHHH
AVDAVKAAGANPQIFGTPTISDGMSMGTEGMKYSLVSREVIADCIETCVQGQWMDGVVVV
HHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
GGCDKNMPGGMIALARLNVPGIYVYGGTIRPGNWKGRDLTIVSSFEAVGEFTAGRMSQED
ECCCCCCCCCEEEEEEECCCEEEEECCEECCCCCCCCEEEEEECHHHHHHHHHCCCCHHH
FEGVEKNACPTSGSCGGMYTANTMSSSFEALGMSLLYSSTMANPDQEKVDSAAESARVLV
HHCHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EAVKRDLKPRDIITKESIENAVSVIMATGGSTNAVLHYLAIAHAAEVDWTIDDFERIRKR
HHHHHCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VPVICDLKPSGQYVATDLHRAGGIPQVLKILLDAGLLHGDCLTITGRTIADELKDVPAVP
CCEEEECCCCCCEEEHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCC
RADQHVIFPIDRALYKEGHLAILKGNLAEDGAVAKITGLKNPVITGPARVFDDEQSAMDA
CCCCEEEEECHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCEECCHHHCCCHHHHHHH
ILGDRIRAGDILVLRYLGPQGGPGMPEMLAPTSAIIGKGLGESVGFITDGRFSGGTWGMV
HHCCCCCCCCEEEEEEECCCCCCCCCHHHCCHHHHHHCCCCCCCCEEECCCCCCCCHHEE
VGHVAPEAFVGGTIALVQEGDSITIDAHRLLLQLNVDEAELARRRAAWRQPAPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHH
AKFAVLARPANQGAVTG
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA