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The map label for this gene is cobS [H]

Identifier: 116690472

GI number: 116690472

Start: 2724348

End: 2725100

Strand: Reverse

Name: cobS [H]

Synonym: Bcen2424_2452

Alternate gene names: 116690472

Gene position: 2725100-2724348 (Counterclockwise)

Preceding gene: 116690473

Following gene: 116690471

Centisome position: 78.22

GC content: 69.59

Gene sequence:

>753_bases
ATGTGGGCCGAACTGCGCTACTTCTTCGTCGCGCTCGGCTATTTCACGCGCGTGCCCGTGCCGCGCTCGATCGGCTACGC
GGCCGGCGATCTCGACCAGGCCGCGCGCTATTTCCCGCTCGTCGGCGCCTGCGTCGGCGCATGGGGCGCGCTCGTCTATC
TCGTCGCACTGCGCGTGCTGCCCGCGTCGATCGCGGTCGGGCTGTCGATGGCCGCGACGCTGCTCGCGACCGGCGCTTTC
CACGAAGACGGCCTCGCCGACAGCTGCGATGCGTTCGGCGGCGGTTACACGCGCGACGACGTGCTGCGCATCATGCACGA
TTCGCGGATCGGCACGTTCGGCGCGGTCGCGCTCGTGATCGCGCTCGGCCTGAAATGGCAGGCGCTGGCGGCGATGCTGC
CGCTGCGCGCCGCATGGACGATGATCGCCGCGCATGCGGCGAGCCGCGCGATGGCCGTGAGCCTGCTGATGTCGCTCGAC
TACGTGCGGCCCGAAGGCAAGGCGAAGCCGGTTGCGCAGCGGATGGGCGCACGCGCGGCGTGCGTCGCGGCGGGGTTCGG
ATTGCCGTGGCTGTTCTGGCCGGACTGGCGCATGGGCGTTGCCGCGTGCGTGGCGCTCATGCTCGTGCGCGCGTGGCTGG
CCCGCTATTTCGTGAAGCGGATCGGCGGTTATACGGGCGACTGTCTCGGCTTCACGCAGCAACTGGGCGAACTGACGATC
TATCTCGTGGTGCTCGGATGGACATCGTCCTGA

Upstream 100 bases:

>100_bases
GCGCTGCCGCTGGTGCGCGCGGCGGCCGCGTTCCTCAGCGAGATGGCGAGCTTCGAGTCCGCCGGCGTCGACAATCGTGA
CGCCTGAGCGCGCGCGCGGC

Downstream 100 bases:

>100_bases
TCCGCCATCCGGCCGTCGGCATCGAGCCGGGCGTCTGCTACGGGCGCAGCGACGTGCCGCTCGCCGATTCGACCGATGGC
GGTGCGCAGGCCGTGCGTGC

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MWAELRYFFVALGYFTRVPVPRSIGYAAGDLDQAARYFPLVGACVGAWGALVYLVALRVLPASIAVGLSMAATLLATGAF
HEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALVIALGLKWQALAAMLPLRAAWTMIAAHAASRAMAVSLLMSLD
YVRPEGKAKPVAQRMGARAACVAAGFGLPWLFWPDWRMGVAACVALMLVRAWLARYFVKRIGGYTGDCLGFTQQLGELTI
YLVVLGWTSS

Sequences:

>Translated_250_residues
MWAELRYFFVALGYFTRVPVPRSIGYAAGDLDQAARYFPLVGACVGAWGALVYLVALRVLPASIAVGLSMAATLLATGAF
HEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALVIALGLKWQALAAMLPLRAAWTMIAAHAASRAMAVSLLMSLD
YVRPEGKAKPVAQRMGARAACVAAGFGLPWLFWPDWRMGVAACVALMLVRAWLARYFVKRIGGYTGDCLGFTQQLGELTI
YLVVLGWTSS
>Mature_250_residues
MWAELRYFFVALGYFTRVPVPRSIGYAAGDLDQAARYFPLVGACVGAWGALVYLVALRVLPASIAVGLSMAATLLATGAF
HEDGLADSCDAFGGGYTRDDVLRIMHDSRIGTFGAVALVIALGLKWQALAAMLPLRAAWTMIAAHAASRAMAVSLLMSLD
YVRPEGKAKPVAQRMGARAACVAAGFGLPWLFWPDWRMGVAACVALMLVRAWLARYFVKRIGGYTGDCLGFTQQLGELTI
YLVVLGWTSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=35.7429718875502, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 26849; Mature: 26849

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEECCCC
>Mature Secondary Structure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EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA