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The map label for this gene is rnfB [H]

Identifier: 116690347

GI number: 116690347

Start: 2590236

End: 2591264

Strand: Direct

Name: rnfB [H]

Synonym: Bcen2424_2326

Alternate gene names: 116690347

Gene position: 2590236-2591264 (Clockwise)

Preceding gene: 116690345

Following gene: 116690348

Centisome position: 74.35

GC content: 70.55

Gene sequence:

>1029_bases
ATGCGGGAAATCGGCGATAATCGAGCTTTGGCCAACCTTTCCGGCCGCGCCCGGCGCGGCCGACCGAGAGCCATTCGGCG
CCGCTGCGCCCGTTGTCAGCCTGTTGTCACCGTGACCGACTCCAAAACACTCGCGGATCGCATCGAAGATCTGCTTCCCC
AGACGCAATGCACGAAGTGCGGCTATAACGGCTGCCGTCCGTACGCCGAGGCGATCGCCGCCGGCGACGCGAACTACAAC
CAGTGCCCGCCCGGCGGCGCCGAAGGCATCGCGCGCCTCGCGAACCTGCTCGGCAAGCCGGTAATTCCGCTGAACCCCGT
CAACGGCAGCGAGCATCCGCGCGCGGTCGCGTTCATCGACGAAAACCTGTGCATCGGCTGCACGCTGTGCATGCAGGCGT
GCCCGGTCGACGCGATCGTCGGCGCGCCGAAGCAGATGCACACGATCGTCGCGTCGCTGTGCACGGGCTGCGACCTGTGC
ATCCCGCCGTGCCCGGTCGACTGCATCGCGATGCTGCCGGTGACCGGCGATCGCACGGGTTGGGATGCGTGGTCGCAGGA
ACAGGCCGACGCCGCGCGCGAACGCCACGACCTGCGGCTCGCGCGCGAGCGCCGCGAGCGCGAGGCCGCCGACGCGCGTG
CTGCCGCCCGCCGCGCGGCCAGTGCCGCCAAGCCGGTTGCGGAGCCCTCTGCAGCGCAGCCCGGTTCGCAGCCGGACACG
CCGGCCGCCGCGCCGGCCGCCGACGACGCCGAAGCGAAGAAACGCGCAATCATCGCCGCCGCACTCGAACGCGCGCGCAA
GAAGAAGGAAGAACTGTCCGGGCAAGGCGCCGGGCCGAAGAATACCGAAGGCGTGAGCGCGGCCGTCCAGGCGCAGATCG
ATGCGGCCGAGGCCCGTCGCAAGCGGCTCGCCGAACAGCAGGCGCAGCGCGATGCCGAAGCCGCATCGGGCAGCAACGAC
CACGACAACGACGCAGGCAACGACGATCCGGACGGCCCGTCCGCGCCGCCCGACCAGAACGCTCCATGA

Upstream 100 bases:

>100_bases
CGCCGGCCGCCCTGGAACGGGCGCGCCGCGGGCGGCCGCCCTCCTGCTGAGCCCCTGCAAAAAAGCGGTTCGATTATGCG
CATAGACGGCGTCCGGCGCA

Downstream 100 bases:

>100_bases
ACGCCAGCAAACGACGCGCGATCTACGAAACGCTGCAGAGCCTCAATCCGCATCCGACCACCGAGCTCGAATATTCGACG
CCGTTCGAGCTGCTGATCGC

Product: ferredoxin

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MREIGDNRALANLSGRARRGRPRAIRRRCARCQPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYN
QCPPGGAEGIARLANLLGKPVIPLNPVNGSEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLC
IPPCPVDCIAMLPVTGDRTGWDAWSQEQADAARERHDLRLARERREREAADARAAARRAASAAKPVAEPSAAQPGSQPDT
PAAAPAADDAEAKKRAIIAAALERARKKKEELSGQGAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAASGSND
HDNDAGNDDPDGPSAPPDQNAP

Sequences:

>Translated_342_residues
MREIGDNRALANLSGRARRGRPRAIRRRCARCQPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYN
QCPPGGAEGIARLANLLGKPVIPLNPVNGSEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLC
IPPCPVDCIAMLPVTGDRTGWDAWSQEQADAARERHDLRLARERREREAADARAAARRAASAAKPVAEPSAAQPGSQPDT
PAAAPAADDAEAKKRAIIAAALERARKKKEELSGQGAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAASGSND
HDNDAGNDDPDGPSAPPDQNAP
>Mature_342_residues
MREIGDNRALANLSGRARRGRPRAIRRRCARCQPVVTVTDSKTLADRIEDLLPQTQCTKCGYNGCRPYAEAIAAGDANYN
QCPPGGAEGIARLANLLGKPVIPLNPVNGSEHPRAVAFIDENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLC
IPPCPVDCIAMLPVTGDRTGWDAWSQEQADAARERHDLRLARERREREAADARAAARRAASAAKPVAEPSAAQPGSQPDT
PAAAPAADDAEAKKRAIIAAALERARKKKEELSGQGAGPKNTEGVSAAVQAQIDAAEARRKRLAEQQAQRDAEAASGSND
HDNDAGNDDPDGPSAPPDQNAP

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=142, Percent_Identity=52.112676056338, Blast_Score=147, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 36051; Mature: 36051

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIGDNRALANLSGRARRGRPRAIRRRCARCQPVVTVTDSKTLADRIEDLLPQTQCTKC
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHC
GYNGCRPYAEAIAAGDANYNQCPPGGAEGIARLANLLGKPVIPLNPVNGSEHPRAVAFID
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
ENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCIPPCPVDCIAMLPVTGDRTG
CCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCC
WDAWSQEQADAARERHDLRLARERREREAADARAAARRAASAAKPVAEPSAAQPGSQPDT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PAAAPAADDAEAKKRAIIAAALERARKKKEELSGQGAGPKNTEGVSAAVQAQIDAAEARR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KRLAEQQAQRDAEAASGSNDHDNDAGNDDPDGPSAPPDQNAP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MREIGDNRALANLSGRARRGRPRAIRRRCARCQPVVTVTDSKTLADRIEDLLPQTQCTKC
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHC
GYNGCRPYAEAIAAGDANYNQCPPGGAEGIARLANLLGKPVIPLNPVNGSEHPRAVAFID
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
ENLCIGCTLCMQACPVDAIVGAPKQMHTIVASLCTGCDLCIPPCPVDCIAMLPVTGDRTG
CCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCC
WDAWSQEQADAARERHDLRLARERREREAADARAAARRAASAAKPVAEPSAAQPGSQPDT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PAAAPAADDAEAKKRAIIAAALERARKKKEELSGQGAGPKNTEGVSAAVQAQIDAAEARR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KRLAEQQAQRDAEAASGSNDHDNDAGNDDPDGPSAPPDQNAP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA