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The map label for this gene is dgkA [H]

Identifier: 116690293

GI number: 116690293

Start: 2526363

End: 2526899

Strand: Direct

Name: dgkA [H]

Synonym: Bcen2424_2272

Alternate gene names: 116690293

Gene position: 2526363-2526899 (Clockwise)

Preceding gene: 116690292

Following gene: 116690294

Centisome position: 72.52

GC content: 70.76

Gene sequence:

>537_bases
TTGAAACGAGACCTGAACGACAAGATCCCGCCCCCCGCCGCGACGCCCTCACGGCACCGCCCGTTCGACGAGGAAGAGCC
GCACGCCGACGCGGACGTGCACCCGCACGAGCCGCTCGGCCCCGACGACCGGCTCGCGCCGTTGCCGCCGAACCCGTACA
AGCGCCACCGCGGCATCACGCGTGCGTGGTTCGCGCTCAAGCATTCGCTGAACGGCTTTCGCGTCGCGATCCGCGAGGAA
AGCGCGTTTCGCCAGGAGCTCACGCTCGCCGCGCTGATGCTGCCGATCGGCGCGTTCGCGCCGGTGCCGGCCGCATCGCG
CGCGCTGCTGATCGGCTCGGTGCTGCTGGTGCTGATCGTCGAGCTGCTGAACTCGAGCGTCGAAGCCGCGATCGACCGCA
TCTCGCTCGAGCGCCACGAACTCTCGAAGCGCGCGAAGGACCTCGGCAGCGCGGCCGTGACGGTCGCGCTGTTCGCGTGC
GTGACGACGTGGGGCTTCGTGCTCGGGCCGGTCGTCGCGCGCTGGCTCGGCTTCTAG

Upstream 100 bases:

>100_bases
GGGCGGAACGCTTCTCGTGGCGCGCGGCATCCGAGCAGTTCGCGTCGCATCTGAAGCCGCTGCCGAAGACCGCGTACTCG
CCAGCCGAAGGTGCCGCCGT

Downstream 100 bases:

>100_bases
GCAACCGCCCGGCGTCCTTCCCGCCGCCCTCATGCGGCGCTGCACCATGCGCCGCGCGGGGCCGCGATCATGAAATGCCC
CGATGTGGCGGAACCCCGGT

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAGK; Diglyceride kinase; DGK [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MKRDLNDKIPPPAATPSRHRPFDEEEPHADADVHPHEPLGPDDRLAPLPPNPYKRHRGITRAWFALKHSLNGFRVAIREE
SAFRQELTLAALMLPIGAFAPVPAASRALLIGSVLLVLIVELLNSSVEAAIDRISLERHELSKRAKDLGSAAVTVALFAC
VTTWGFVLGPVVARWLGF

Sequences:

>Translated_178_residues
MKRDLNDKIPPPAATPSRHRPFDEEEPHADADVHPHEPLGPDDRLAPLPPNPYKRHRGITRAWFALKHSLNGFRVAIREE
SAFRQELTLAALMLPIGAFAPVPAASRALLIGSVLLVLIVELLNSSVEAAIDRISLERHELSKRAKDLGSAAVTVALFAC
VTTWGFVLGPVVARWLGF
>Mature_178_residues
MKRDLNDKIPPPAATPSRHRPFDEEEPHADADVHPHEPLGPDDRLAPLPPNPYKRHRGITRAWFALKHSLNGFRVAIREE
SAFRQELTLAALMLPIGAFAPVPAASRALLIGSVLLVLIVELLNSSVEAAIDRISLERHELSKRAKDLGSAAVTVALFAC
VTTWGFVLGPVVARWLGF

Specific function: Recycling of diacylglycerol produced during the turnover of membrane phospholipid [H]

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

Organism=Escherichia coli, GI1790475, Length=111, Percent_Identity=50.4504504504504, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 19540; Mature: 19540

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDLNDKIPPPAATPSRHRPFDEEEPHADADVHPHEPLGPDDRLAPLPPNPYKRHRGIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHH
RAWFALKHSLNGFRVAIREESAFRQELTLAALMLPIGAFAPVPAASRALLIGSVLLVLIV
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ELLNSSVEAAIDRISLERHELSKRAKDLGSAAVTVALFACVTTWGFVLGPVVARWLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRDLNDKIPPPAATPSRHRPFDEEEPHADADVHPHEPLGPDDRLAPLPPNPYKRHRGIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHH
RAWFALKHSLNGFRVAIREESAFRQELTLAALMLPIGAFAPVPAASRALLIGSVLLVLIV
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ELLNSSVEAAIDRISLERHELSKRAKDLGSAAVTVALFACVTTWGFVLGPVVARWLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]