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The map label for this gene is nuoM [H]

Identifier: 116690258

GI number: 116690258

Start: 2490743

End: 2492233

Strand: Reverse

Name: nuoM [H]

Synonym: Bcen2424_2237

Alternate gene names: 116690258

Gene position: 2492233-2490743 (Counterclockwise)

Preceding gene: 116690259

Following gene: 116690257

Centisome position: 71.54

GC content: 62.11

Gene sequence:

>1491_bases
ATGCACGCTTTTCCGATTCTCAGTACCGCGATCTGGCTGCCGATCGTTTTCGGCCTCCTCGTGCTGGCGGTAGGTAACGA
CAAAAATCCGGGGACGGCCCGCTGGGTTGCGCTGATCGGCGCGCTGCTCGGCCTCGCGGTCACGATCCCGTTGATCACGG
GCTTCGACTCGAGCACGGCCGCACTGCAGTTCGTCGAGAAGTCGACCTGGATCGAACGCTTCGACATTGCGTATCACCTC
GGCGTCGACGGCATCTCGATGTGGTTCGTCGTGCTGACCGCGCTGATCACGGTGATCGTCGTGATCGCCGCATGGGAAGT
GATCACCGAGAACGTCGCGCAGTACCTCGCGGCATTCCTGATCCTGTCCGGGATCATGATCGGCGTGTTCTCGGCGGCCG
ACGGCCTGCTGTTCTACGTGTTCTTCGAGGCGACCCTGATCCCGATGTACATCATCATCGGCGTGTGGGGCGGCCCGAAC
CGCGTGTATGCGGCATTCAAGTTCTTCCTGTACACGCTGGCCGGCTCGCTGCTGATGCTGGTCGCGCTGATCTACCTGTA
CACGGAAACGCATTCGTTCGACCTCGCGACGTGGCAGAACGCGAAGATCGCGATGACGCCACAGATCTTGCTGTTCATTG
CCTTCTTCCTCGCGTTCGCGGTGAAGGTGCCGATGTGGCCGGTGCACACGTGGCTGCCGGACGCGCACGTGGAAGCGCCG
ACAGGCGGCTCGGTCGTGCTGGCTGCGATTATGCTGAAGCTCGGCGCATACGGTTTCCTGCGCTTCTCGCTGCCGATCAC
GCCTGACGCGAGCCACTTCCTGGCACCCGTCGTGATCACGCTGTCGCTGATCGCGGTGATCTACATCGGCCTCGTCGCGA
TGGTGCAGGCCGACATGAAGAAGCTGGTCGCGTATTCGTCGATCGCGCACATGGGCTTCGTCACGCTCGGCTTCTTCATC
TTCAACCAGCTCGGCGTCGAAGGCGCGATCATCCAGATGATCTCGCACGGCTTCGTGTCGGGCGCGATGTTCCTCTGCAT
CGGCGTGTTGTACGACCGCGTGCACTCGCGCCAGATCGCCGACTACGGCGGCGTCGTGAACGTGATGCCGAAGTTCGCGG
CATTCGCGATGCTGTTCTCGATGGCCAACTGCGGCCTGCCGGGTACGTCGGGTTTCGTCGGCGAGTTCATGGTGATTCTC
GCGGCCGTCCAGTACAACTTCTGGATCGCATTCGGCGCGGCATTCACGCTGATCCTCGGCGCGGCCTACACGCTGTGGAT
GTACAAGCGCGTGTATTTCGGCGCGGTGGCGAACGATCACGTCGCCAAGCTGAAGGACATCGGCCGTCGCGAATTCCTGA
TGCTGGCCGTGCTCGCCGCGTTCACGCTGCTGATGGGCCTGTATCCGAAGCCTTTCACCGACGTGATGCACGTTTCCGTC
GAAAACCTCCTCTCCCACGTCGCGCAGTCGAAGCTGCCGCTGGCCCAGTAA

Upstream 100 bases:

>100_bases
TTACATCTATCACTACGCGTTCGCCATGATCATCGGCATGCTGGGGCTCCTGACCCTGTTTGTAACGCTCGGCGGCAAAT
AAGGCGGGGAGACAACACTA

Downstream 100 bases:

>100_bases
TCGCGAGCGGAGGAACTCAAGATCATGAACGCTCCTATGAATGTCCTGTTGCCCGACGCGCTGGTGATGGCCGCCATCGT
CGTCGCATGGCTGAACGACA

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGTARWVALIGALLGLAVTIPLITGFDSSTAALQFVEKSTWIERFDIAYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ

Sequences:

>Translated_496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGTARWVALIGALLGLAVTIPLITGFDSSTAALQFVEKSTWIERFDIAYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ
>Mature_496_residues
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGTARWVALIGALLGLAVTIPLITGFDSSTAALQFVEKSTWIERFDIAYHL
GVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFLILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPN
RVYAAFKFFLYTLAGSLLMLVALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMKKLVAYSSIAHMGFVTLGFFI
FNQLGVEGAIIQMISHGFVSGAMFLCIGVLYDRVHSRQIADYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVIL
AAVQYNFWIAFGAAFTLILGAAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
ENLLSHVAQSKLPLAQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=371, Percent_Identity=34.7708894878706, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=24.0409207161125, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1788613, Length=469, Percent_Identity=39.4456289978678, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788827, Length=379, Percent_Identity=23.7467018469657, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788831, Length=316, Percent_Identity=25, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788829, Length=443, Percent_Identity=26.86230248307, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI145693160, Length=451, Percent_Identity=23.7250554323725, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788614, Length=383, Percent_Identity=25.8485639686684, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54141; Mature: 54141

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGTARWVALIGALLGLAVTIPLITGFDSSTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALQFVEKSTWIERFDIAYHLGVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFL
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIIQMISHGFVSGAMFLCIGVLYDRVHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAFGAAFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLAQ
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHAFPILSTAIWLPIVFGLLVLAVGNDKNPGTARWVALIGALLGLAVTIPLITGFDSSTA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALQFVEKSTWIERFDIAYHLGVDGISMWFVVLTALITVIVVIAAWEVITENVAQYLAAFL
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILSGIMIGVFSAADGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLAGSLLML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VALIYLYTETHSFDLATWQNAKIAMTPQILLFIAFFLAFAVKVPMWPVHTWLPDAHVEAP
HHHHHHHHCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
TGGSVVLAAIMLKLGAYGFLRFSLPITPDASHFLAPVVITLSLIAVIYIGLVAMVQADMK
CCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLVAYSSIAHMGFVTLGFFIFNQLGVEGAIIQMISHGFVSGAMFLCIGVLYDRVHSRQIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYGGVVNVMPKFAAFAMLFSMANCGLPGTSGFVGEFMVILAAVQYNFWIAFGAAFTLILG
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYTLWMYKRVYFGAVANDHVAKLKDIGRREFLMLAVLAAFTLLMGLYPKPFTDVMHVSV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ENLLSHVAQSKLPLAQ
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]