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The map label for this gene is puuA [H]

Identifier: 116690184

GI number: 116690184

Start: 2409597

End: 2410934

Strand: Reverse

Name: puuA [H]

Synonym: Bcen2424_2163

Alternate gene names: 116690184

Gene position: 2410934-2409597 (Counterclockwise)

Preceding gene: 116690185

Following gene: 116690183

Centisome position: 69.2

GC content: 64.65

Gene sequence:

>1338_bases
ATGCAACCCGAACTGAGCGAATTCCTGCGACAGCACCGCATCACCGAAGTCGAGGCGATCATTCCCGACATGGCCGGCAT
CGCGCGCGGCAAGATCATTCCGCGCAACAAGTTCGAATCCGGCGAATCGATGCGCCTGCCACAGGCCGTGATGGTGCAGA
CCGTCACCGGCGACTATCCGGAGGACGGCACGCTGACCGGCGTGACCGATCCCGACATGGTGTGCGTGCCCGATCCGTCG
ACGATCTGCCTGATCCCGTGGGCGGTCGATCCGACCGCGCAGGTGATCCACGATTGCGTGCATTTCGACGGCTCGCCGGT
CGAGATTTCGCCGCGCTACGTGCTGCGCCGCGTGCTCGACCTGTACGAGGCGAAGGGCTGGAAACCCGTGGTCGCGCCCG
AGCTCGAGTTTTATCTGGTCGACATGAACGCCGACCCCGACCTGCCGCTGCGGCCGCCCGTCGGCCGCACGGGGCGGGCG
GAAACGGGCCGCCAGTCGTATTCGATCGAGGCCGTCAACGAGTTCGATCCGCTGTTCGAGGACATCTACGAGTACTGCGA
AATGCAGGGGCTCGATATCGAGACGCTGATTCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCGTGCATGGCGACG
CGCTGCCGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACGGTGCGCGAGGCCGCGCTGCGTCACAACATGTATGCGACG
TTCATGGCCAAGCCGATGGAAAACGAGCCGGGTTCCGCGATGCACATTCATCAGAGCCTCGCGGACATGCGCACCGGGCG
CAATCTGTTCGCCGACGAAAACGGCGCCGTGTCGCCGCTGTTCCGCAGCTATCTGGCCGGGCTGCAGAAGTACACGCCGG
CGCTGATGCCGATCTTCGCGCCGTACATCAATTCGTACCGCCGCCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAG
TGGGGCTACGACAACCGCACGGTGGGCTTTCGCATTCCGCAGTCGAGCCCCGTCGCGCGCCGCATCGAGAACCGGATTCC
GGGCGTCGACTGCAACCCGTACCTCGCGTTCGCGGCGACGCTCGCCGCCGGCTATCTCGGCATGACGCAACAGCTCGCGC
CAACCGAGCCGATCGCATCGGACGGCTACGACCTGCCGTACCAGCTGCCGCGCAACCTGGAGGAAGGCATCTCGCTGATG
GCCGCGTGCGAGCCGCTTGCCGGCATCCTCGGCGACAAGTTCGTGAAGGCCTATCTGGCATTGAAGGAAACCGAATACGA
AGCGTTCTTCCGCGTGATCAGCTCGTGGGAACGCAGGCACCTGCTGCTGCACGTGTAA

Upstream 100 bases:

>100_bases
CGGCGTGCCGCGCGCGCATGACGCACCGCATGCATGCTGCCGGCGGTGCGATACCGTCGCCGGCCGCATCCGACATCGAC
ATCGACCAACGAGGCCGATC

Downstream 100 bases:

>100_bases
GCGTGCGGCGCTGTTTGACGGAGAAAACATGACCGATCGAAACGAAGCTTTTCCGCCGCGCTCGACCGCCGATTTTCGCG
CGCTCGATGCCGCCCACCAC

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYEAKGWKPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALPLADQVFLFKRTVREAALRHNMYAT
FMAKPMENEPGSAMHIHQSLADMRTGRNLFADENGAVSPLFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSSPVARRIENRIPGVDCNPYLAFAATLAAGYLGMTQQLAPTEPIASDGYDLPYQLPRNLEEGISLM
AACEPLAGILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYEAKGWKPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALPLADQVFLFKRTVREAALRHNMYAT
FMAKPMENEPGSAMHIHQSLADMRTGRNLFADENGAVSPLFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSSPVARRIENRIPGVDCNPYLAFAATLAAGYLGMTQQLAPTEPIASDGYDLPYQLPRNLEEGISLM
AACEPLAGILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYEAKGWKPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDPLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALPLADQVFLFKRTVREAALRHNMYAT
FMAKPMENEPGSAMHIHQSLADMRTGRNLFADENGAVSPLFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSSPVARRIENRIPGVDCNPYLAFAATLAAGYLGMTQQLAPTEPIASDGYDLPYQLPRNLEEGISLM
AACEPLAGILGDKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=435, Percent_Identity=25.9770114942529, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=35.1893095768374, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=29.8056155507559, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49975; Mature: 49975

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLD
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYEAKGWKPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFE
HHHCCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALPLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMENEPGSAMHIHQSLADMRTGRNLFADENGAVSPLFRSYLAGLQKYTPALMPIFA
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSSPVARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQQLAPTEPIASDGYDLPYQLPRNLEEGISLMAACEPLAGILGDKFVKAYLA
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLD
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYEAKGWKPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFE
HHHCCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALPLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMENEPGSAMHIHQSLADMRTGRNLFADENGAVSPLFRSYLAGLQKYTPALMPIFA
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSSPVARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQQLAPTEPIASDGYDLPYQLPRNLEEGISLMAACEPLAGILGDKFVKAYLA
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]