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The map label for this gene is eno [H]

Identifier: 116690128

GI number: 116690128

Start: 2336617

End: 2337900

Strand: Reverse

Name: eno [H]

Synonym: Bcen2424_2107

Alternate gene names: 116690128

Gene position: 2337900-2336617 (Counterclockwise)

Preceding gene: 116690129

Following gene: 116690127

Centisome position: 67.11

GC content: 64.02

Gene sequence:

>1284_bases
ATGAGTGCAATCGTAGATATCATCGGCCGCGAGATTCTGGATTCGCGCGGCAACCCCACCGTCGAATGCGACGTGCTGCT
CGAATCGGGCACGATGGGCCGCGCGGCGGTGCCGTCGGGCGCGTCCACCGGCTCGCGTGAAGCGATCGAACTGCGCGACG
GCGAAGCCGGCCGCTACAACGGCAAGGGCGTGCTGAAGGCAGTCGAGCACATCAACACCGAAATCTCCGAAGCCATCATG
GGCCTCGACGCGTCGGAACAGGCGTTCCTCGACAAGACGCTGCTCGAGCTGGACGGCACCGACAACAAGTCGCGCCTGGG
CGCGAACGCGATGCTGGCCGTGTCGATGGCCGTCGCGAAGGCCGCCGCCGAAGAGGCCGGCCTGCCGCTGTACCGCTACT
TCGGCGGGTCGGGTGCGATGCAACTGCCGGTGCCGATGATGAACATCGTCAACGGCGGCGCGCACGCGAACAACAGCCTG
GACATCCAGGAATTCATGATCGTTCCGGTCAGCCAGCCGACGTTCCGTGAAGCGCTGCGTTGCGGCGCGGAAGTGTTCCA
CGCGCTGAAGAAGATCCTGAGCGACCGCGGCATGAGCACGGCCGTCGGCGACGAAGGCGGTTTCGCGCCGAACTTCGGCA
GCAACGACGAGTGCCTGTCGACGATCCTGCAGGCGATCGAGAAGGCCGGCTACCGTGCGGGCGAAGACGTGCTGCTCGCG
CTCGACTGCGCGGCATCCGAGTTCTACCACGACGGCAAGTACCAGCTCGCCGGCGAAGGCCTGCAACTGTCGTCGGCCGA
ATTCACCGACTACCTCGCGACGCTCGCCGACAAGTTCCCGATCGTGTCGATCGAGGACGGCATGCACGAAAGCGACTGGG
ACGGCTGGAAGCTGCTGACCGATCGCCTCGGCAAGAAGGTGCAGCTGGTCGGCGACGACCTGTTCGTCACGAACACGCGC
ATCCTGAAGGAAGGCATCGAGAAGGGCATCGCGAACTCGATCCTCATCAAGATCAACCAGATCGGTACGCTGACCGAAAC
GTTCGCGGCGATCGAAATGGCGAAGCGCGCGGGCTACACGGCCGTGATCTCGCACCGCTCGGGCGAAACGGAAGATTCGA
CGATCGCGGACATCGCGGTCGGCCTGAACGCCGGTCAGATCAAGACGGGTTCGCTGTCGCGCAGCGACCGCATTTCGAAG
TACAACCAGCTGCTGCGCATCGAGGAAGATCTCGGCGACATCGCGAGCTACCCGGGCAAATCGGCTTTCTATAACCTGCG
CTAA

Upstream 100 bases:

>100_bases
AATGAACCTTGCGACGGCTTCGCGCGTCTGTAGCGAAAGCGGTTCCGGCACGCACGCCGTCGCAGTTTGAAGAATCCATC
GTCATTCTCAGAGGAAACCC

Downstream 100 bases:

>100_bases
CGCGCTACTATCGGGTTCGTCATCTCGACGCCGCTCTGTGCCTGTGCGCGGAGCGGCGCTTCTTATATCTGCGTCTCTTA
CATGCGGCTTGTCACTGTCG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYNGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPTFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHESDWDGWKLLTDRLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR

Sequences:

>Translated_427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYNGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPTFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHESDWDGWKLLTDRLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR
>Mature_426_residues
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYNGKGVLKAVEHINTEISEAIMG
LDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLD
IQEFMIVPVSQPTFREALRCGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLAL
DCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHESDWDGWKLLTDRLGKKVQLVGDDLFVTNTRI
LKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKY
NQLLRIEEDLGDIASYPGKSAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=51.9450800915332, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.4199535962877, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=49.1990846681922, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=49.1990846681922, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=44.6261682242991, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.7857142857143, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.7857142857143, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.7857142857143, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=66.3529411764706, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=49.6567505720824, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=49.6567505720824, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=44.6700507614213, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=46.3133640552995, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.2222222222222, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.2222222222222, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.2222222222222, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=47.0046082949309, Blast_Score=355, Evalue=7e-99,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=49.7663551401869, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=49.7663551401869, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=49.7663551401869, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=49.7663551401869, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=49.7663551401869, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=49.7663551401869, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45687; Mature: 45556

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYN
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPTFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHESDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
DRLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC
>Mature Secondary Structure 
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYN
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPTFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILSDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHESDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
DRLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA