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The map label for this gene is gltX [H]

Identifier: 116690077

GI number: 116690077

Start: 2281923

End: 2283332

Strand: Reverse

Name: gltX [H]

Synonym: Bcen2424_2056

Alternate gene names: 116690077

Gene position: 2283332-2281923 (Counterclockwise)

Preceding gene: 116690079

Following gene: 116690076

Centisome position: 65.54

GC content: 66.67

Gene sequence:

>1410_bases
ATGACCCGTCCTGTCCGTACCCGCTTCGCGCCGAGTCCCACCGGCTTCATCCACCTCGGCAACATCCGCTCCGCACTCTA
TCCGTGGGCGTTCGCCCGCAAGATGAAAGGCACGTTCGTGCTGCGCATCGAGGATACCGACGTCGAGCGCTCGTCGCAGG
AAGCGGTCGATGCGATCCTCGAGGGGATGCAGTGGCTCGGACTGGATTTCGACGAAGGCCCGATCTACCAGATGCAGCGG
ATGGACCGCTATCGCGAAGTGCTCGCGCAGATGCTCGAGAAGGGTCTCGCATATCCGTGCTACATGTCGGCCGAGGAACT
CGACGCGCTGCGCGAACGCCAGCGCGAAGCCGGCCTGAAGCCGCGCTACGACGGTACGTGGCGCCCGGAGCCCGGCAAGG
TGCTGCCGGAGCCGCCGGCCGGCGTGAAGCCGGTGCTGCGCTTCCGCAATCCGCTGACGGGCACGGTCGTGTGGGACGAT
GCGGTGAAGGGGCGTGTCGAGATCTCGAACGAGGAGCTCGACGACCTCGTGATCGCGCGCCCGGACGGCACGCCGATCTA
CAACTTCTGCGTGGTGGTGGACGACATGGACATGGGCATCACGCACGTGATCCGCGGTGACGACCACGTGAACAACACGC
CGCGCCAGATCAACATCCTGAACGCACTCGGCGGCGAGCCGCCCGTCTATGCGCACTTGCCGACCGTGCTGAACGAGCAG
GGCGAGAAGATGAGCAAGCGGCATGGCGCGATGAGCGTGATGGCGTACCGCGACGCGGGCTTCCTGCCGGAAGCCGTCGT
CAACTATCTCGCGCGTCTCGGCTGGTCGCACGGCGACGCCGAGATCTTCTCGCGCGAGCAGTTCGTCGAATGGTTCGATC
TCGAGCATCTCGGCAAGTCGCCCGCGCAGTACGACCACAGCAAGCTGAGCTGGCTGAACGCGCATTACATCAAGGAAGCC
GACAACGCGCGCCTTGCCGAACTCGCGAAGCCGTTCCTCGACGCACTCGGCATCGACGACGCGGCGATCGCGACGGGCCC
CGCGCTCGACGCGGTGGTCGGCTTGATGAAGGATCGTGCGACGACGGTCAAGGAGATCGCGGAAGGTGCGGCGATGTTCT
ATCGTGTGCCGGCACCCGACGCCGACGCGCTCGCGCAGCACGTGACCGATGCGGTGCGTCCGGCGCTGGCCGATCTCGCG
GCCGCGCTGAAGGCGGCCGACTGGACGAAGGAAGCGGTTTCCGCCGCGCTGAAGGCGACGCTCGCCACGCACAAGCTGAA
GATGCCGCAGCTCGCGATGCCGGTGCGTCTGCTGGTGGCGGGCACGACGCATACGCCGTCGATCGACGCGGTGCTCGTGC
TGTTCGGTCGCGACGTCGTGGTGACGCGCATCGAGGCCGCGCTGGCTTGA

Upstream 100 bases:

>100_bases
TCTTTCCCGTTCCGGGCGCCGCCGGCTCCGTTGCTGTCCGGGCTGCCGCGTCGTCCCGCGTCGAATCATTCATGCGCCCC
AGGCGTTCGAGTTACCGTTT

Downstream 100 bases:

>100_bases
CGTTCGTGCGCGCGCGAAGCTGTCCGTGCATGAGGTACGCGGCGGCTTCGCAAAAAATTTCGCACGAATCGTAAAAAGGG
TATTTACAAGTTCGAAATCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMQWLGLDFDEGPIYQMQR
MDRYREVLAQMLEKGLAYPCYMSAEELDALRERQREAGLKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDD
AVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEA
DNARLAELAKPFLDALGIDDAAIATGPALDAVVGLMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLA
AALKAADWTKEAVSAALKATLATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDVVVTRIEAALA

Sequences:

>Translated_469_residues
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMQWLGLDFDEGPIYQMQR
MDRYREVLAQMLEKGLAYPCYMSAEELDALRERQREAGLKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDD
AVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEA
DNARLAELAKPFLDALGIDDAAIATGPALDAVVGLMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLA
AALKAADWTKEAVSAALKATLATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDVVVTRIEAALA
>Mature_468_residues
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILEGMQWLGLDFDEGPIYQMQRM
DRYREVLAQMLEKGLAYPCYMSAEELDALRERQREAGLKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDA
VKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQG
EKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEAD
NARLAELAKPFLDALGIDDAAIATGPALDAVVGLMKDRATTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLAA
ALKAADWTKEAVSAALKATLATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDVVVTRIEAALA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=330, Percent_Identity=36.0606060606061, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=49.7854077253219, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=37.1428571428571, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786895, Length=207, Percent_Identity=25.6038647342995, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=29.1666666666667, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17543332, Length=130, Percent_Identity=30, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=420, Percent_Identity=32.3809523809524, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.6094420600858, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=123, Percent_Identity=32.520325203252, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=493, Percent_Identity=30.0202839756592, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17864488, Length=302, Percent_Identity=24.5033112582781, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51839; Mature: 51708

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMQWLGLDFDEGPIYQMQRMDRYREVLAQMLEKGLAYPCYMSAEELDALRERQREAGLK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEHHHCCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHSKLSWLNAHYIKEADNARLAELAKPFLDALGIDDAAIATGPALDAVVGLMKDRA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
TTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLAAALKAADWTKEAVSAALKAT
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDVVVTRIEAALA
HHHHHCCCCCHHCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
TRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
EGMQWLGLDFDEGPIYQMQRMDRYREVLAQMLEKGLAYPCYMSAEELDALRERQREAGLK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPIYNFCVVVDDMDMGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQ
CCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEHHHCCCCCCEEEECCHHHHHH
GEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHSKLSWLNAHYIKEADNARLAELAKPFLDALGIDDAAIATGPALDAVVGLMKDRA
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
TTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLAAALKAADWTKEAVSAALKAT
HHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDVVVTRIEAALA
HHHHHCCCCCHHCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA