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The map label for this gene is alkK [H]

Identifier: 116689928

GI number: 116689928

Start: 2114771

End: 2116480

Strand: Reverse

Name: alkK [H]

Synonym: Bcen2424_1907

Alternate gene names: 116689928

Gene position: 2116480-2114771 (Counterclockwise)

Preceding gene: 116689934

Following gene: 116689927

Centisome position: 60.75

GC content: 67.49

Gene sequence:

>1710_bases
ATGGCTTCCGTACGGAAACCGTCCCCTGGCTGGTCACCGATTCGCATCCTCAGGGAGAACAAGACGATGGGTAAGCCGTT
GCTGGGCCAGATGATGGATGTGCCGCTGCTGGTGTCGTCGCTGATTTCGCATGCCGCGCGGCACGCCGGCGACACGGAGA
TCGTGTCGAAGCGCGTGGAAGGCGACCTGCATCGCTATACGTACCGCGATTGCGAACGCCGCGCGAAGCAGCTGGCGCAG
GCGCTCGCGCGGCTCGGCGTCGAGACCGGCGACCGCGTCGGCACGCTGGCGTGGAACGGCTACCGGCACCTGGAAGCGTA
TTACGGGATCGGCGGGATGGGCGCCGTGTGCCACACGATCAACCCGCGCCTGTTTCCCGAACAGATCGCGTACATCGTCA
ATCACGCGGAAGACCGCTACGTCTTCTTCGACATCAATTTCGCGCCGCTCGTCGATGCGATCGCGCCGCAATGCCCGCAC
GTGAAGGGCTGGATCGCGATGACGGACGCCGCGCACCTGCCGTCCGGCGCGACGCCGTTCCTCTGCTACGAAACGCTCGT
CGAAGCCGAGGACGGGCGCTACGACTGGCCGCGGCTCGACGAGCAGCAGGCGTCGGGGCTCTGCTACACGTCGGGCACGA
CCGGCAACCCGAAGGGCGTGCTGTATTCGCATCGCTCGACCGTGCTGCATGCTTACGGCGCCGCGCTGCCCGACGCAATG
AACCTGTCCGCGATGGACGCCGTGCTGCCCGTCGTGCCGATGTTCCACGTCAACGCGTGGGGGCTGCCGTACGCGGTGCC
GCTGACGGGCGGCAAGCTCGTGCTGCCCGGCAAGGATCTCGACGGGAAATCGCTGTACGAGCTGATGGAGGCCGAGCGCG
TGACGTTCTCCGCGGGCGTGCCGACCGTGTGGCTCGGCCTGCTGAACTACATGCGCGAGGCCGGCGTGCGTTTCTCGACG
CTGAACCGCACGGTGATCGGCGGCTCCGCGTGCCCGCCCGCGATGCTGCGCACGTTCGAGGACGAATACGGCGTGCGCGT
GATCCATGCGTGGGGGATGACCGAACTGTCGCCGCTCGGCACGCTCGCGAAGCTCAACTGGGCGCAGTCGCAGCGGCCGC
TCGACGCGCAGCGCCGGCTGCTCGAGAAGCAGGGGCGCGTGATCTGCGGCGTCGACATGCGGATCGTCGGCGACGACGGC
CGCGAGCTGCCGTGGGACGGTGTCGCATTCGGCGAACTGCAGGTGCGCGGGCCGTGGGTGATCGACCACTACTTTCGCGG
CGATGCGTCGCCGCTGGCGGACGGCTGGTTTCCGACCGGCGACGTCGCGACGATCGACCCCGACGGCTTTCTGCAGATCA
CCGACCGCAGCAAGGACGTGATCAAGTCGGGCGGCGAGTGGATCAGCTCGATCGACATCGAGAACGTCGCGATCGCGCAT
CCCGGGGTGGCCGAAGCCGCGTGCATCGCGTGTGCGCACCCGAAATGGACCGAGCGGCCGCTGCTCGTCGTGGTGCCGCG
CGAAGGCGCGAACCTGACCCGCGACACGCTGCTCGCGTTCTACGAGGGCAAGGTCGCGAAATGGTGGATTCCGGACGACG
TCGTGTTCGTCGAATCGTTGCCGCACACCGCGACCGGCAAGCTGCAGAAGCTGAAGTTGCGAGAGACGTTCCGCGATTAC
GTGTTGCCCACGGCGGTGTGCGCGCCGTAA

Upstream 100 bases:

>100_bases
TGGGTAAGCCGATATTGTAAGGCGGCGCCGCGCCGCCCGCATGGCGGCCCGCGCACCGTGCGCCACGCCCGTCCGGGGCC
GCTGACGGGCGCACACCGGG

Downstream 100 bases:

>100_bases
GGCCCAAGCCCGGGTCGCGGCCGGCCCGCGTATCCGTCGCCCGATGCCGGTCGCCCGATCCGTCCCCGGATCGGGAGCAA
AATTCAAATTGAACGACCGT

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MASVRKPSPGWSPIRILRENKTMGKPLLGQMMDVPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQ
ALARLGVETGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVDAIAPQCPH
VKGWIAMTDAAHLPSGATPFLCYETLVEAEDGRYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAM
NLSAMDAVLPVVPMFHVNAWGLPYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFST
LNRTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRRLLEKQGRVICGVDMRIVGDDG
RELPWDGVAFGELQVRGPWVIDHYFRGDASPLADGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAH
PGVAEAACIACAHPKWTERPLLVVVPREGANLTRDTLLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDY
VLPTAVCAP

Sequences:

>Translated_569_residues
MASVRKPSPGWSPIRILRENKTMGKPLLGQMMDVPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQ
ALARLGVETGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVDAIAPQCPH
VKGWIAMTDAAHLPSGATPFLCYETLVEAEDGRYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAM
NLSAMDAVLPVVPMFHVNAWGLPYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFST
LNRTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRRLLEKQGRVICGVDMRIVGDDG
RELPWDGVAFGELQVRGPWVIDHYFRGDASPLADGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAH
PGVAEAACIACAHPKWTERPLLVVVPREGANLTRDTLLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDY
VLPTAVCAP
>Mature_568_residues
ASVRKPSPGWSPIRILRENKTMGKPLLGQMMDVPLLVSSLISHAARHAGDTEIVSKRVEGDLHRYTYRDCERRAKQLAQA
LARLGVETGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTINPRLFPEQIAYIVNHAEDRYVFFDINFAPLVDAIAPQCPHV
KGWIAMTDAAHLPSGATPFLCYETLVEAEDGRYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAMN
LSAMDAVLPVVPMFHVNAWGLPYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGVPTVWLGLLNYMREAGVRFSTL
NRTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLGTLAKLNWAQSQRPLDAQRRLLEKQGRVICGVDMRIVGDDGR
ELPWDGVAFGELQVRGPWVIDHYFRGDASPLADGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAHP
GVAEAACIACAHPKWTERPLLVVVPREGANLTRDTLLAFYEGKVAKWWIPDDVVFVESLPHTATGKLQKLKLRETFRDYV
LPTAVCAP

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=527, Percent_Identity=26.9449715370019, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI187761345, Length=525, Percent_Identity=26.0952380952381, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI187761343, Length=525, Percent_Identity=26.0952380952381, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI38505220, Length=446, Percent_Identity=24.8878923766816, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI28416953, Length=568, Percent_Identity=24.4718309859155, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.9844357976654, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI58082049, Length=549, Percent_Identity=22.4043715846995, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157311624, Length=549, Percent_Identity=21.8579234972678, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI157311622, Length=549, Percent_Identity=21.8579234972678, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI122937307, Length=510, Percent_Identity=21.7647058823529, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=25.763358778626, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI145693145, Length=502, Percent_Identity=26.8924302788845, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788107, Length=529, Percent_Identity=26.4650283553875, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1786810, Length=356, Percent_Identity=28.3707865168539, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789201, Length=372, Percent_Identity=26.8817204301075, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=565, Percent_Identity=23.8938053097345, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=565, Percent_Identity=23.8938053097345, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=565, Percent_Identity=23.8938053097345, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=523, Percent_Identity=26.1950286806883, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=25.78125, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=25.1417769376182, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=22.5925925925926, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=24.2990654205607, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=22.3300970873786, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=24.3243243243243, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=502, Percent_Identity=21.9123505976096, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=503, Percent_Identity=22.4652087475149, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=380, Percent_Identity=22.3684210526316, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=27.007299270073, Blast_Score=165, Evalue=9e-41,
Organism=Drosophila melanogaster, GI18859661, Length=471, Percent_Identity=25.4777070063694, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922652, Length=502, Percent_Identity=24.7011952191235, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=22.5621414913958, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17933690, Length=502, Percent_Identity=23.3067729083665, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356441, Length=509, Percent_Identity=22.3968565815324, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648260, Length=517, Percent_Identity=24.5647969052224, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648257, Length=504, Percent_Identity=22.2222222222222, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648253, Length=557, Percent_Identity=21.3644524236984, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358303, Length=476, Percent_Identity=22.6890756302521, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648255, Length=551, Percent_Identity=21.0526315789474, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356947, Length=504, Percent_Identity=21.8253968253968, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=20, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 62681; Mature: 62549

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00139 THIOL_PROTEASE_CYS ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVRKPSPGWSPIRILRENKTMGKPLLGQMMDVPLLVSSLISHAARHAGDTEIVSKRVE
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GDLHRYTYRDCERRAKQLAQALARLGVETGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEHHHHHCCCCCHHHHHHHC
NPRLFPEQIAYIVNHAEDRYVFFDINFAPLVDAIAPQCPHVKGWIAMTDAAHLPSGATPF
CCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHH
LCYETLVEAEDGRYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAM
HHHHHHHHCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCC
NLSAMDAVLPVVPMFHVNAWGLPYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGV
CHHHHHHHHHHHHHEEECCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCC
PTVWLGLLNYMREAGVRFSTLNRTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLG
HHHHHHHHHHHHHCCCEEEECCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
TLAKLNWAQSQRPLDAQRRLLEKQGRVICGVDMRIVGDDGRELPWDGVAFGELQVRGPWV
HHHHCCHHHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEE
IDHYFRGDASPLADGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAH
EEHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCHHHHHCCCCEEHHCCCCCEEECC
PGVAEAACIACAHPKWTERPLLVVVPREGANLTRDTLLAFYEGKVAKWWIPDDVVFVESL
CCCHHHHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCEEEEECC
PHTATGKLQKLKLRETFRDYVLPTAVCAP
CCCCCCCHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
ASVRKPSPGWSPIRILRENKTMGKPLLGQMMDVPLLVSSLISHAARHAGDTEIVSKRVE
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
GDLHRYTYRDCERRAKQLAQALARLGVETGDRVGTLAWNGYRHLEAYYGIGGMGAVCHTI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEHHHHHCCCCCHHHHHHHC
NPRLFPEQIAYIVNHAEDRYVFFDINFAPLVDAIAPQCPHVKGWIAMTDAAHLPSGATPF
CCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHH
LCYETLVEAEDGRYDWPRLDEQQASGLCYTSGTTGNPKGVLYSHRSTVLHAYGAALPDAM
HHHHHHHHCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCC
NLSAMDAVLPVVPMFHVNAWGLPYAVPLTGGKLVLPGKDLDGKSLYELMEAERVTFSAGV
CHHHHHHHHHHHHHEEECCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHEEEECCC
PTVWLGLLNYMREAGVRFSTLNRTVIGGSACPPAMLRTFEDEYGVRVIHAWGMTELSPLG
HHHHHHHHHHHHHCCCEEEECCEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCC
TLAKLNWAQSQRPLDAQRRLLEKQGRVICGVDMRIVGDDGRELPWDGVAFGELQVRGPWV
HHHHCCHHHCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCEE
IDHYFRGDASPLADGWFPTGDVATIDPDGFLQITDRSKDVIKSGGEWISSIDIENVAIAH
EEHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCHHHHHCCCCEEHHCCCCCEEECC
PGVAEAACIACAHPKWTERPLLVVVPREGANLTRDTLLAFYEGKVAKWWIPDDVVFVESL
CCCHHHHHEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCEEEEECC
PHTATGKLQKLKLRETFRDYVLPTAVCAP
CCCCCCCHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]