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The map label for this gene is potI [H]

Identifier: 116689769

GI number: 116689769

Start: 1944284

End: 1945105

Strand: Reverse

Name: potI [H]

Synonym: Bcen2424_1748

Alternate gene names: 116689769

Gene position: 1945105-1944284 (Counterclockwise)

Preceding gene: 116689770

Following gene: 116689765

Centisome position: 55.83

GC content: 65.09

Gene sequence:

>822_bases
ATGAAGCCGAATCGCTACCTGCAGTTCGCGGCGCTGTTCGTCGGTTTCGCGTTCCTGTACATCCCGATCATCAGCCTGAT
CGTCTATTCGTTCAACGAGTCGAAGCTCGTCACCGTGTGGTCGGGCTTCTCGTTCCGCTGGTACGCGGCGCTCGTCGAGG
ACGACGAGCTGCTGACGGCCGCGTGGCTGTCGCTGAAGATCGGCGTGCTGACCGCGTTTGCGTCGGTGTTCATCGGCACG
TGGGCCGGCTTCGTGCTCGCACGGATGGGACGCTTTCGCGGCTTCGCGCTGTTCAGCGGGATGATCAACGCGCCGCTCGT
GATTCCCGAGGTGATCCAGGGGATCTCGCTGCTGCTGCTGTTCATCGAGCTCGCGAAGTGGATCGGCTGGCCGGCCGAGC
GCGGCGTGTTCACGATCTGGCTCGGCCACGTGATGCTGTGCATCTCGTACGTCGCGATCATCGTGCAGTCGCGCGTGCGC
GAGCTGAATCCGTCGCTGGAAGAGGCCGCGCTCGATCTCGGCGCGACCCCGCTGAAGGTGTTCTTCACGATCACGCTGCC
GCTGATCTCGCAGGCGCTGATCGCGGGCTGGCTGCTGTCGTTCACGCTGTCGATCGACGATCTGGTGCTATCGGCGTTCC
TGTCGGGCCCCGGCTCGACGACGCTGCCGCTCGTCGTGTTCTCGCGCGTGCGCCTTGGCCTGAACCCGGAGATGAACGCG
CTCGCGACGCTGTTCATCATCGCGGTGACGGCCGGCGTCGTGATCGCGAACTTCGTGATGCTGCGTCAGGAGCGCAAGCG
GCTGGCGGGGTTCGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCTGCCGTGACCTGCGCGATGGTGTTGCTGCTGCTCGTGCCGATGGCGATGTTCCAGCACTTCCAGGCG
AAGGAACAGGGAGGCCGTCG

Downstream 100 bases:

>100_bases
CACGCCGATTGCGGCCATCATGAAAAACGCCCGGTGCCGACGACGAGTCGGGCCGGGCGTTTCGTTTTGGCGGCGCGCAA
CGATCGCCGGCGCGTCGCCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKPNRYLQFAALFVGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYAALVEDDELLTAAWLSLKIGVLTAFASVFIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIIAVTAGVVIANFVMLRQERKRLAGFAA

Sequences:

>Translated_273_residues
MKPNRYLQFAALFVGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYAALVEDDELLTAAWLSLKIGVLTAFASVFIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIIAVTAGVVIANFVMLRQERKRLAGFAA
>Mature_273_residues
MKPNRYLQFAALFVGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYAALVEDDELLTAAWLSLKIGVLTAFASVFIGT
WAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVR
ELNPSLEEAALDLGATPLKVFFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIIAVTAGVVIANFVMLRQERKRLAGFAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=257, Percent_Identity=59.5330739299611, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=38.2716049382716, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787714, Length=248, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788764, Length=214, Percent_Identity=30.8411214953271, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30072; Mature: 30072

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPNRYLQFAALFVGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYAALVEDDELLTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
AWLSLKIGVLTAFASVFIGTWAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIIAVTAGVVIANFVMLRQERKRLAGFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPNRYLQFAALFVGFAFLYIPIISLIVYSFNESKLVTVWSGFSFRWYAALVEDDELLTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
AWLSLKIGVLTAFASVFIGTWAGFVLARMGRFRGFALFSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAERGVFTIWLGHVMLCISYVAIIVQSRVRELNPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALIAGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIIAVTAGVVIANFVMLRQERKRLAGFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]