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The map label for this gene is shiA [H]

Identifier: 116689274

GI number: 116689274

Start: 1383513

End: 1384823

Strand: Direct

Name: shiA [H]

Synonym: Bcen2424_1252

Alternate gene names: 116689274

Gene position: 1383513-1384823 (Clockwise)

Preceding gene: 116689272

Following gene: 116689275

Centisome position: 39.71

GC content: 67.66

Gene sequence:

>1311_bases
ATGACTCAGTCCCTTCCTTCCGCCCGCCAGCCGGCACGCGCCGCGACGGCCGCGTTCATCGGCACGATGATCGAGTGGTA
CGACTTCTACATCTACGCGACCGCCGCCGCGCTCGTGTTCGGCGAACTCTATTTTCCGTCGCATGACCCGTTCGTCAGCA
CGATGGCATCGTTCGCGACGTTCGCGGTCGGCTTCTTCGCGCGTCCGCTCGGCGGCGTGATCTTCGGCCATCTCGGCGAC
CGCATCGGCCGCAAGAAGGCGCTGATGACGACGTTGATGATGATGGGCGTCGCGACCGTGTGCGTCGGGCTGCTGCCCGC
TTATTCGAAGGTCGGCATCCTCGCGCCGGTGCTGCTCGTCGCGCTGCGCGTCGTGCAGGGCATCGCGGTGGGCGGCGAAT
GGGGCGGCGCGGTGCTGATGGCGGGCGAGCATGCGCCGCAGGGGCGCCGCACGTTTTTCGCGTCGTTCGCGCAGCTCGGC
AGCCCGGCCGGGCTGATCCTGTCGCTGATTGCGTTCCGCGCGGTCACGTCGATCGACAAGGCCGACTTCCTCGCGTGGGG
CTGGCGCCTGCCGTTTCTCGCGAGTTCGGTGCTGCTCGTGGTCGGCATCCTGATCCGGCTCGGCGTGAACGAGTCGCCGG
AATTCGCGCGGGTGAAGGAAGCCAACCGCACGGTGAAGCTGCCGGTCGCGGAAGTGTTCCGTTCCGCGTCGGGGCTCGTG
CTGCTCTGCATCGGCGCGAATACGATCGGCATCGCGGGCGTCTATTTCACGAACACGTTCATGATCGCGTACACGACGCA
GTACGTCGGCGTGTCGCGGTCGCTGATCCTCGACAGCCTGTTCGCGGTCGCGATCATCCAGTTTTTCGCGCAGCCGCTCG
CCGCCTGGATCGCCGAACGTATTGGCGGCGCGCGCTTCCTCAAGCTCGCGGCGCTGCTCGCGATGCTGTCGCCGTATCCG
ATGTTCATGCTCGTGCAGGGCGGCACGTCCGCGTCGCTGATCGCCGGCATCGCGCTCGCCGTGGTCTGCATGGCCGGCTT
CTATTCGGTGATCGCGGGCTTCGTCAGCGACGTGTTTCCCGCGCACGTGCGCTATTCGGCGATCTCGCTCGCTTACCAGA
TTTGCGGCGCGATCGCGGGCGGCCTGACGCCGCTCGTCGGCACGTGGCTCGCACATCGCTTCACGGGCCAGTGGTGGCCG
CTCGCGGTGTTCTACACGTGTCTCGCCGGTATTTCGCTGCTCTGCATCGTCGCGCTCGATGCGCGCCGCGCCGCGCGGCC
GGCCGCCGCCGAAGCGCTCGGCTCGCACTGA

Upstream 100 bases:

>100_bases
ATAAAGGAGGGGCGCCGCAGCGTGCGGCGCGAGGCCGCGACAGACGGCCGCCGTCACGATATTGCGTGCTGCCCCGCTGC
GGCGCGCGTCAGGAGCCCGC

Downstream 100 bases:

>100_bases
CCTTCAACGAACGAATCGATCCGGAATGCATATGAAAGACCTGCTGGAAATCGACGGCGCCCGCCTGTGGCAGAGCCTGA
CCGACATGGCGCGCGTCGGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDPFVSTMASFATFAVGFFARPLGGVIFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPAYSKVGILAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLIAFRAVTSIDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSASGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFFAQPLAAWIAERIGGARFLKLAALLAMLSPYP
MFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFTGQWWP
LAVFYTCLAGISLLCIVALDARRAARPAAAEALGSH

Sequences:

>Translated_436_residues
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDPFVSTMASFATFAVGFFARPLGGVIFGHLGD
RIGRKKALMTTLMMMGVATVCVGLLPAYSKVGILAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLG
SPAGLILSLIAFRAVTSIDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSASGLV
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFFAQPLAAWIAERIGGARFLKLAALLAMLSPYP
MFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFTGQWWP
LAVFYTCLAGISLLCIVALDARRAARPAAAEALGSH
>Mature_435_residues
TQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDPFVSTMASFATFAVGFFARPLGGVIFGHLGDR
IGRKKALMTTLMMMGVATVCVGLLPAYSKVGILAPVLLVALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGS
PAGLILSLIAFRAVTSIDKADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSASGLVL
LCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFFAQPLAAWIAERIGGARFLKLAALLAMLSPYPM
FMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFPAHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFTGQWWPL
AVFYTCLAGISLLCIVALDARRAARPAAAEALGSH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=39.2111368909513, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=32.258064516129, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=33.2535885167464, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788942, Length=380, Percent_Identity=29.2105263157895, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=23.2067510548523, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322632, Length=359, Percent_Identity=25.3481894150418, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46215; Mature: 46084

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDPFVSTMASFAT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGVIFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPAYSKVGILAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLIAFRAVTSIDK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSASGLV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHCCCCCEE
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFFAQPLAAWIAER
EEEECCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGARFLKLAALLAMLSPYPMFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFTGQWWPLAVFYTCLAGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAARPAAAEALGSH
HHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TQSLPSARQPARAATAAFIGTMIEWYDFYIYATAAALVFGELYFPSHDPFVSTMASFAT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGVIFGHLGDRIGRKKALMTTLMMMGVATVCVGLLPAYSKVGILAPVLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRVVQGIAVGGEWGGAVLMAGEHAPQGRRTFFASFAQLGSPAGLILSLIAFRAVTSIDK
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCH
ADFLAWGWRLPFLASSVLLVVGILIRLGVNESPEFARVKEANRTVKLPVAEVFRSASGLV
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEECCHHHHHCCCCCEE
LLCIGANTIGIAGVYFTNTFMIAYTTQYVGVSRSLILDSLFAVAIIQFFAQPLAAWIAER
EEEECCCCHHHHHHHHHHEEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGARFLKLAALLAMLSPYPMFMLVQGGTSASLIAGIALAVVCMAGFYSVIAGFVSDVFP
HCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHVRYSAISLAYQICGAIAGGLTPLVGTWLAHRFTGQWWPLAVFYTCLAGISLLCIVALD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARRAARPAAAEALGSH
HHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]