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The map label for this gene is otsA [H]

Identifier: 116689180

GI number: 116689180

Start: 1267839

End: 1269224

Strand: Direct

Name: otsA [H]

Synonym: Bcen2424_1158

Alternate gene names: 116689180

Gene position: 1267839-1269224 (Clockwise)

Preceding gene: 116689179

Following gene: 116689182

Centisome position: 36.39

GC content: 67.53

Gene sequence:

>1386_bases
ATGAGCCGGCTCATCATCGTTTCAAACCGCGTCGCGCCGATTTCGGAAGGCGAACCGGCGGCGGGCGGCCTCGCGATCGG
CGTCTATGATGCGCTGAAGGAAACCGGCGGCATGTGGTTCGGCTGGAGCGGCGAAGTGGTCGCGTCCGGCGCGCCGCAGC
CGCAGATCCGCATCGAGGAGCGCGGGCCGGTGACGTTCGCGACCGTCGGGCTGACGCGCCGCGATTACGACCAGTATTAC
CGCGGGTTCTCGAACGCGACGCTGTGGCCCGCGTTCCATTACCGCGCGGACCTGATCCAGTACGATCGCCACGAATTCGA
CGGCTACCGCCGCGTCAACGTCTGGCTCGCGCAGCAGCTCGTGCCGCTGCTGCAGGACGACGACGTGATCTGGGTGCACG
ACTATCACCTGATTCCGTTCGCGCGCGCGCTGCGCGACGCCGGCGTGAAGAACCGCATCGGCTTCTTCCTGCACATCCCG
TTTCCGGCCGCGCAGGTGCTCGTCAACGTGCCGCCGCACCGAGAGCTCGTCGAGTCGCTGTGCGCGTTCGATCTGCTCGG
CTTCCAGACCGAGCCCGACCTGCGCGCGTTTTGCGACTACGTCGAGTTCGAGGCGGACGGCGAAGTCGAGCACGACGGGC
ACACGGTGCGCGTACGGGCGTTCGGCCAGACGTTGCGCGCGGCCGCCTATCCGATCGGCGTGTATCCGGACGAGATCGCG
TCGCTCGCGCAGGCGGGTGAGCACGGCAAGGCGGTGCGCACGCTCGCGACGTCGCTGCGCGGGCGGCAACTGATCATGAG
CGTCGACCGGCTCGATTATTCGAAGGGGCTCGTCGAGCGCTTCCGGGCCTTCGAGAAGCTGCTCGAGCACCAGGCGTCGA
TCCGCAACCGCGTGTCGTTCCTGCAGATCGCACCGTCGACGCGTGCGGACCTGCGCGCGTACCAGGACATCCGCCTGCAG
CTCGAAGCGGAGTCGGGGCGCATCAACGGGCGCTACGCGGAGCTCGACTGGGCGCCGATCCTCTACATTCACCGTCAGTA
CGACCGTCAGGTGCTGGCCGCGCTGTACCGGCTCGCGCGCGTGGGCTTCGTCACGCCGCTGCGCGACGGGATGAACCTCG
TCGCGAAGGAGTACGTGTCCGCGCAGAATCCGGACGATCCGGGCGTGCTGGTGCTGTCGCGCTTCGCGGGCGCCGCGCGT
GAGCTCACGGGCGCGCTGATCGTCAATCCGATCGATATCGACGGGATGGCCGATGCGCTGTCACAGGCGCTGACGATGTC
GCTCACCGAGCGGCGTGCGCGTTACGCGGACATGATCGCGCAGCTTCGCGAGAACAACGTGTCGGTGTGGCGGGACAATT
TCCTGCGTGATCTGCAGCAGGTCTGA

Upstream 100 bases:

>100_bases
AGGTCGGCGCGGGCGAGACGTCCGCGCGCATGCGGCTCGATTCGGTCGACGCGCTGCATGAGCAGATCGCAGGCTGGCTC
GGTGCGGGGCAACCGCACGC

Downstream 100 bases:

>100_bases
TCTGATTTATTGCTTGTGCTGCAATTGAAAAAGCCGCCGTGCGCAAGCACGGCGGCTTTTTTGTCGGCGGATACCGGTGC
GTCAGGCCACGCGTTCGCCG

Product: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQPQIRIEERGPVTFATVGLTRRDYDQYY
RGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRDAGVKNRIGFFLHIP
FPAAQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYVEFEADGEVEHDGHTVRVRAFGQTLRAAAYPIGVYPDEIA
SLAQAGEHGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASIRNRVSFLQIAPSTRADLRAYQDIRLQ
LEAESGRINGRYAELDWAPILYIHRQYDRQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAAR
ELTGALIVNPIDIDGMADALSQALTMSLTERRARYADMIAQLRENNVSVWRDNFLRDLQQV

Sequences:

>Translated_461_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQPQIRIEERGPVTFATVGLTRRDYDQYY
RGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRDAGVKNRIGFFLHIP
FPAAQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYVEFEADGEVEHDGHTVRVRAFGQTLRAAAYPIGVYPDEIA
SLAQAGEHGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASIRNRVSFLQIAPSTRADLRAYQDIRLQ
LEAESGRINGRYAELDWAPILYIHRQYDRQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAAR
ELTGALIVNPIDIDGMADALSQALTMSLTERRARYADMIAQLRENNVSVWRDNFLRDLQQV
>Mature_460_residues
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQPQIRIEERGPVTFATVGLTRRDYDQYYR
GFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRDAGVKNRIGFFLHIPF
PAAQVLVNVPPHRELVESLCAFDLLGFQTEPDLRAFCDYVEFEADGEVEHDGHTVRVRAFGQTLRAAAYPIGVYPDEIAS
LAQAGEHGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASIRNRVSFLQIAPSTRADLRAYQDIRLQL
EAESGRINGRYAELDWAPILYIHRQYDRQVLAALYRLARVGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARE
LTGALIVNPIDIDGMADALSQALTMSLTERRARYADMIAQLRENNVSVWRDNFLRDLQQV

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=462, Percent_Identity=53.030303030303, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI115533560, Length=427, Percent_Identity=30.4449648711944, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI115533558, Length=427, Percent_Identity=30.4449648711944, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71987012, Length=428, Percent_Identity=28.2710280373832, Blast_Score=141, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6319602, Length=471, Percent_Identity=30.5732484076433, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6323537, Length=392, Percent_Identity=26.2755102040816, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320279, Length=427, Percent_Identity=25.7611241217799, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323917, Length=377, Percent_Identity=26.525198938992, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.9794238683128, Blast_Score=269, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52015; Mature: 51884

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQPQIRIEE
CCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCCCCEEEEC
RGPVTFATVGLTRRDYDQYYRGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQL
CCCEEEEEECCCHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
VPLLQDDDVIWVHDYHLIPFARALRDAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVESL
HHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHH
CAFDLLGFQTEPDLRAFCDYVEFEADGEVEHDGHTVRVRAFGQTLRAAAYPIGVYPDEIA
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHH
SLAQAGEHGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASIRNRVSF
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LQIAPSTRADLRAYQDIRLQLEAESGRINGRYAELDWAPILYIHRQYDRQVLAALYRLAR
EEECCCCHHHHHHHEEEEEEEEECCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
VGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARELTGALIVNPIDIDGMADAL
HCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHHCCEEEEECCCCCHHHHHH
SQALTMSLTERRARYADMIAQLRENNVSVWRDNFLRDLQQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQPQIRIEE
CCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCCCCEEEEC
RGPVTFATVGLTRRDYDQYYRGFSNATLWPAFHYRADLIQYDRHEFDGYRRVNVWLAQQL
CCCEEEEEECCCHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
VPLLQDDDVIWVHDYHLIPFARALRDAGVKNRIGFFLHIPFPAAQVLVNVPPHRELVESL
HHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCHHHHHHHH
CAFDLLGFQTEPDLRAFCDYVEFEADGEVEHDGHTVRVRAFGQTLRAAAYPIGVYPDEIA
HHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHH
SLAQAGEHGKAVRTLATSLRGRQLIMSVDRLDYSKGLVERFRAFEKLLEHQASIRNRVSF
HHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
LQIAPSTRADLRAYQDIRLQLEAESGRINGRYAELDWAPILYIHRQYDRQVLAALYRLAR
EEECCCCHHHHHHHEEEEEEEEECCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
VGFVTPLRDGMNLVAKEYVSAQNPDDPGVLVLSRFAGAARELTGALIVNPIDIDGMADAL
HCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHHCCEEEEECCCCCHHHHHH
SQALTMSLTERRARYADMIAQLRENNVSVWRDNFLRDLQQV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA