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The map label for this gene is moaA [H]

Identifier: 116689132

GI number: 116689132

Start: 1220283

End: 1221395

Strand: Reverse

Name: moaA [H]

Synonym: Bcen2424_1109

Alternate gene names: 116689132

Gene position: 1221395-1220283 (Counterclockwise)

Preceding gene: 116689133

Following gene: 116689131

Centisome position: 35.06

GC content: 69.18

Gene sequence:

>1113_bases
ATGTCCCGACGCATCATTCCTCTCGCCGACGTCAGCGGGATGCCGGACGTCTCCGGCGTCGCGCATGCCCCCGACGGCAC
GCTGGCCGACACGTTCGCCAGGCCGCTGCGCGACCTGCGCATTTCGGTGACGGACCGCTGCAACTTCCGTTGCGTGTACT
GCATGCCGCGCGCGGTGTTCGACAAGGACTACCCGTTCCTGCCGCACAGCGCGCTGCTCACGCACGAGGAAATCGAGCGC
GTGGCGCGGCTCTTCGTCGCGCACGGCGTCGAGAAGATCCGCATCACGGGCGGTGAGCCGTTGCTGCGCAAGAACCTCGA
ATTCCTGATCGAGCGCCTCGCGCGTCTGACGACGCATGCCGGCCGCCCGCTCGACCTGACGCTGACGACCAACGGCTCGC
TGCTCGCACGCAAGGCGCGCGCGCTGAAGGACGCCGGGCTCACGCGCGTCACGGTCAGTCTCGATGCGCTCGACGACGCG
CTGTTCAAGCGCATGAACGATGCCGAGTTCGCGAGCGCCGACGTGCTCGACGGCATCTTCGCCGCGCAGGCCGCGGGCCT
CGCGCCGGTCAAGGTCAACATGGTCGTGAAGCGCGGCACCAACGACAGCGAGATCCTGCCGATGGCCGAGCGCTTCCGCG
GCACCGGCGTGATCCTGCGTTTCATCGAATACATGGACGTCGGCACGTCGAACGGCTGGAACATGACCGAGGTACTGCCG
TCGGCGGACGTCGTCGCGCGCATCGCCGAACATTTCCCGCTCGTGCCGCTCGAGCCGCACACGGCGGCCGAAACCGCGCA
GCGCTGGGCCTATGCGGACGGCAGCGGCGAGATCGGCGTGATCTCGAGCGTCACGCAGGCCTTCTGCGGCGACTGCACGC
GCGCGCGGCTGTCGACCGAAGGCAAGCTGTACCTGTGCCTGTTCGCGTCGACCGGCCACGACCTGCGCGCACTCGTGCGC
GGCGGCGCGAGCGATGCCGAGATCGCGACCGCGATCGCCCGCATCTGGCAGGCGCGCACCGACCGCTATTCGCAGCTGCG
CGGCAGCGCATCGGCCGATACCGCGCCCGACGGCGCCGGCAAGCGCGTCGAAATGTCGTATATCGGCGGCTGA

Upstream 100 bases:

>100_bases
CTCCGGCGGGCTTTTTCACGTCCGCGCTGCGGGTTTCCGCCCACCGGCCCCGCCGGCCGGTTGCGGCTAAAATAGCAGTC
TGGTTCGCACTTCTTCCAAC

Downstream 100 bases:

>100_bases
CGCACGCCGCTCTGTCCACCCTGCGCACGATGCCCGCCCCCGCCTTCCCGTCAATTGCCGGCCTGCTGCTCGCAGGCGGG
CGCGCCACCCGCATGGATGG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSRRIIPLADVSGMPDVSGVAHAPDGTLADTFARPLRDLRISVTDRCNFRCVYCMPRAVFDKDYPFLPHSALLTHEEIER
VARLFVAHGVEKIRITGGEPLLRKNLEFLIERLARLTTHAGRPLDLTLTTNGSLLARKARALKDAGLTRVTVSLDALDDA
LFKRMNDAEFASADVLDGIFAAQAAGLAPVKVNMVVKRGTNDSEILPMAERFRGTGVILRFIEYMDVGTSNGWNMTEVLP
SADVVARIAEHFPLVPLEPHTAAETAQRWAYADGSGEIGVISSVTQAFCGDCTRARLSTEGKLYLCLFASTGHDLRALVR
GGASDAEIATAIARIWQARTDRYSQLRGSASADTAPDGAGKRVEMSYIGG

Sequences:

>Translated_370_residues
MSRRIIPLADVSGMPDVSGVAHAPDGTLADTFARPLRDLRISVTDRCNFRCVYCMPRAVFDKDYPFLPHSALLTHEEIER
VARLFVAHGVEKIRITGGEPLLRKNLEFLIERLARLTTHAGRPLDLTLTTNGSLLARKARALKDAGLTRVTVSLDALDDA
LFKRMNDAEFASADVLDGIFAAQAAGLAPVKVNMVVKRGTNDSEILPMAERFRGTGVILRFIEYMDVGTSNGWNMTEVLP
SADVVARIAEHFPLVPLEPHTAAETAQRWAYADGSGEIGVISSVTQAFCGDCTRARLSTEGKLYLCLFASTGHDLRALVR
GGASDAEIATAIARIWQARTDRYSQLRGSASADTAPDGAGKRVEMSYIGG
>Mature_369_residues
SRRIIPLADVSGMPDVSGVAHAPDGTLADTFARPLRDLRISVTDRCNFRCVYCMPRAVFDKDYPFLPHSALLTHEEIERV
ARLFVAHGVEKIRITGGEPLLRKNLEFLIERLARLTTHAGRPLDLTLTTNGSLLARKARALKDAGLTRVTVSLDALDDAL
FKRMNDAEFASADVLDGIFAAQAAGLAPVKVNMVVKRGTNDSEILPMAERFRGTGVILRFIEYMDVGTSNGWNMTEVLPS
ADVVARIAEHFPLVPLEPHTAAETAQRWAYADGSGEIGVISSVTQAFCGDCTRARLSTEGKLYLCLFASTGHDLRALVRG
GASDAEIATAIARIWQARTDRYSQLRGSASADTAPDGAGKRVEMSYIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=306, Percent_Identity=37.9084967320261, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI116734668, Length=306, Percent_Identity=37.9084967320261, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1786999, Length=348, Percent_Identity=34.4827586206897, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32566331, Length=330, Percent_Identity=31.5151515151515, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17561210, Length=319, Percent_Identity=32.9153605015674, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI32566329, Length=319, Percent_Identity=31.6614420062696, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28574488, Length=347, Percent_Identity=33.4293948126801, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28574490, Length=322, Percent_Identity=35.0931677018634, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 40158; Mature: 40027

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRIIPLADVSGMPDVSGVAHAPDGTLADTFARPLRDLRISVTDRCNFRCVYCMPRAVF
CCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCEEEEECCHHHH
DKDYPFLPHSALLTHEEIERVARLFVAHGVEKIRITGGEPLLRKNLEFLIERLARLTTHA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCC
GRPLDLTLTTNGSLLARKARALKDAGLTRVTVSLDALDDALFKRMNDAEFASADVLDGIF
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAQAAGLAPVKVNMVVKRGTNDSEILPMAERFRGTGVILRFIEYMDVGTSNGWNMTEVLP
HHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCC
SADVVARIAEHFPLVPLEPHTAAETAQRWAYADGSGEIGVISSVTQAFCGDCTRARLSTE
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHCCHHHHHCCCC
GKLYLCLFASTGHDLRALVRGGASDAEIATAIARIWQARTDRYSQLRGSASADTAPDGAG
CCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KRVEMSYIGG
CEEEEEECCC
>Mature Secondary Structure 
SRRIIPLADVSGMPDVSGVAHAPDGTLADTFARPLRDLRISVTDRCNFRCVYCMPRAVF
CCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCEEEEECCHHHH
DKDYPFLPHSALLTHEEIERVARLFVAHGVEKIRITGGEPLLRKNLEFLIERLARLTTHA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCC
GRPLDLTLTTNGSLLARKARALKDAGLTRVTVSLDALDDALFKRMNDAEFASADVLDGIF
CCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAQAAGLAPVKVNMVVKRGTNDSEILPMAERFRGTGVILRFIEYMDVGTSNGWNMTEVLP
HHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHCC
SADVVARIAEHFPLVPLEPHTAAETAQRWAYADGSGEIGVISSVTQAFCGDCTRARLSTE
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHCCHHHHHCCCC
GKLYLCLFASTGHDLRALVRGGASDAEIATAIARIWQARTDRYSQLRGSASADTAPDGAG
CCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
KRVEMSYIGG
CEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]