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The map label for this gene is trmD [H]

Identifier: 116689094

GI number: 116689094

Start: 1184590

End: 1185384

Strand: Direct

Name: trmD [H]

Synonym: Bcen2424_1071

Alternate gene names: 116689094

Gene position: 1184590-1185384 (Clockwise)

Preceding gene: 116689093

Following gene: 116689095

Centisome position: 34.0

GC content: 66.42

Gene sequence:

>795_bases
ATGAACCAGGTTACCGAGAGCGCGGTGCAGTTCGACGTCGTCACGCTCTTTCCCGAGATGTTCCGTGCACTGACCGACTG
GGGAATCACCAGCCGGGCCGTCAAGCAGGGGCGCTTCGGGTTGCGCACCTGGAACCCGCGTGATTTCACGACGGACAACT
ACCGCACGGTCGACGATCGTCCGTACGGCGGTGGTCCGGGCATGGTGATGCTGGCCAGGCCGCTCGAAGCTGCAATCGAC
GCCGCGAAAGCGGCGCAGGCGGAGCAGGGCATCGCGAGCACGCGCGTCGTGATGATGTCGCCGCAAGGCGCGCCGCTCAC
GCACGATCGCGCGGTGCGGATGGCGCAGGAACCCGGCGTCGTCGTGCTGTGCGGTCGCTACGAAGCGATCGACCAGCGCC
TGCTCGACCGTTGCGTCGACGAGGAAATCAGCCTCGGCGATTTCGTGCTGTCGGGCGGCGAGCTGCCGGCGATGGCAATG
ATGGATGCAGTCGTGCGGTTGCTGCCCGGCGTGCTGAACGATTCGTTGTCGGCCGTGCAGGACAGCTTCGCCGATGGCCT
GCTCGATTGTCCGCACTACACGCGCCCCGAGGAATACGACGGTGTACGCGTGCCGGACGTGCTGCTCGGCGGGCATCATG
CCGAGATCGAGAAGTGGCGGCGCCAGGAAGCATTGAGGAATACGTTGCGGAAGCGGCCGGACCTGATCGTCCGGGCGCGC
CGCGAAAAGTTGTTGAGCCGGGCCGACGAGGCCTGGCTCGCGAACCTCGCACGCGAAGCGAAGGACGCCTCCTGA

Upstream 100 bases:

>100_bases
ACCACCGACGAGCGGTTGATTCCGTTCGTCGGTGTCTACGTCAAAACGGTGGATCAGGCGGCGCGTCGCATCGTCGTCGA
CTGGGAAGCCGATTACTGAA

Downstream 100 bases:

>100_bases
GGCGGCTGCGCGAGCGGGAATTGGCGGTGCCGTACATGCGTGTGCGGCGCCTGATGTGAATCCATCCTCTATCGGGGCCA
GGCCTGCAAGCAGGCGGGTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAID
AAKAAQAEQGIASTRVVMMSPQGAPLTHDRAVRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDSLSAVQDSFADGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEKWRRQEALRNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS

Sequences:

>Translated_264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAID
AAKAAQAEQGIASTRVVMMSPQGAPLTHDRAVRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDSLSAVQDSFADGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEKWRRQEALRNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS
>Mature_264_residues
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLARPLEAAID
AAKAAQAEQGIASTRVVMMSPQGAPLTHDRAVRMAQEPGVVVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAM
MDAVVRLLPGVLNDSLSAVQDSFADGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEKWRRQEALRNTLRKRPDLIVRAR
REKLLSRADEAWLANLAREAKDAS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=54.4642857142857, Blast_Score=252, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29333; Mature: 29333

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLARPLEAAIDAAKAAQAEQGIASTRVVMMSPQGAPLTHDRAVRMAQEPGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCE
VVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDSLSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEKWRRQEALRNTLRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REKLLSRADEAWLANLAREAKDAS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQVTESAVQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLARPLEAAIDAAKAAQAEQGIASTRVVMMSPQGAPLTHDRAVRMAQEPGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCE
VVLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAMMDAVVRLLPGVLNDSLSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEKWRRQEALRNTLRKRPDLIVRAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
REKLLSRADEAWLANLAREAKDAS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA