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The map label for this gene is gatA [H]

Identifier: 116689045

GI number: 116689045

Start: 1130626

End: 1132002

Strand: Reverse

Name: gatA [H]

Synonym: Bcen2424_1022

Alternate gene names: 116689045

Gene position: 1132002-1130626 (Counterclockwise)

Preceding gene: 116689046

Following gene: 116689044

Centisome position: 32.49

GC content: 72.98

Gene sequence:

>1377_bases
ATGACCACTTTCACGCCGTTCCCCCCGCTCGCCCAGCTCGCCGCCGACCTCGCCGCCGGCCGGACCACGAGCCGCGCGCT
CGTCGACACCGCGCTCGACCGGATCGCCGATCCGTCGGGCCAGGGCGCCGTCGTCTTCACCGAAGTCGATGCGGACAATG
CCCGCGCGGCCGCCGACGCACACGACCGGCTGCGCGCCGCGGGCACCGTGCTGTCGCCGCTCGCGGGCATTCCGGTGTCG
GTCAAGGACCTGTTCGACGTCGCGGGCCAGGTCACGCGCGCGGGGTCGCGCGTGCTCGACGGCGCGCCGGCCGCGCGCAC
CGATGCGGTCGCCGTCGCGCGGCTCAAGCGGGCGGGCGCGGTGCTGGTCGGCCGCACCAACATGAGCGAATTCGCGTTCT
CCGGGCTCGGGCTGAATCCGCACTTCGGCAACCCGCGCTCGCCGTATCACCGCGACGTGCCAGGCGACGCGCGGATTTCG
GGCGGCTCGTCGTCGGGAGCGGCCGCGTCGGTCGCCGACGGGATGGCCGCCGTCGCGCTCGGCACCGACACCGGCGGCTC
GATCCGCATTCCGGCCGCGCTGTGCGGGCTGACGGGCTTCAAACCGACCGCGAGCCGAATTCCGACGCAAGGCGGCGTGC
CGCTGTCGACGACGCTCGACTCGTTCGGCCCGATCGGCCTGACGGTCGCGTGCTGCGCACTCGTCGACCGGATGCTGGCG
GGGCTCGAGCCGCACGTGCCGGCCGCCCGGCCGCTCGAAGGTGTACGGCTCGGCGTGCTGACGAACTACGTGACGGACGG
CGTCGATGCCGACGTCGCGGCCGCGCTCGACACCGCGCTCAAGCATCTCGAAGCCGCCGGCGCGATCGTCACCGAAGTGC
GCTTCCCCGCGCTCGACCGGCTGCCGGAGATCAACCGTTTCGGCTTCTCGCCGATCGAGGCGTACGCGTGGCATCGTCCG
CTGCTCGCCACGCATCGCGACCAGTACGACCCGCGCGTGCTGACGCGCATCCTGAAGGGCGAACCGGCGAGCGCGGCCGA
CTATCTCGACCTGCTCGTCGCACGCGCCGCGATGCTCGACGAAGCCGCGCACACGGTCTGGTCGCGCTTCGATGCGCTCG
TCGCGCCGACGGTGCCGGTCGTGCCGCCGCGCATCGCCGAACTCGAAACGGACGACGACGCGTTCACGCGCACCAACGCG
CTGATCCTGCGCAACCCGAGCGCGTTCAACTTCCTCGATGCGTGCGGGCTGTCGCTGCCGTGCCATCCGCGCGACGCGGC
GCCGGTCGGCCTGATGCTCGCGGCGGCGCCGCATCGCGACGACGCGCTGCTCGCGATCGGCCAGTCGGTCGAGGCCGTGC
TGAACACGATCCGCTGA

Upstream 100 bases:

>100_bases
GCTGTCCGCACGCGACGCCGCCGGCGCGCGCGCGGCCGTCGTAGGCCATATCGATTCGTTCCGCTCCACGCTTCTTCAAC
ATCTTCGCCCCTGAGCCGCC

Downstream 100 bases:

>100_bases
CCGCGCGAGCCGATGGCAAGCGGAGCGGCGGCGGGCCGCGCCCGCGTGCGGCAGGGTGCCGCACGCGCGCTGTCAGTTTC
GTGCCAGACCGTTCGCGCTA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVS
VKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGNPRSPYHRDVPGDARIS
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFPALDRLPEINRFGFSPIEAYAWHRP
LLATHRDQYDPRVLTRILKGEPASAADYLDLLVARAAMLDEAAHTVWSRFDALVAPTVPVVPPRIAELETDDDAFTRTNA
LILRNPSAFNFLDACGLSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR

Sequences:

>Translated_458_residues
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVS
VKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGNPRSPYHRDVPGDARIS
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFPALDRLPEINRFGFSPIEAYAWHRP
LLATHRDQYDPRVLTRILKGEPASAADYLDLLVARAAMLDEAAHTVWSRFDALVAPTVPVVPPRIAELETDDDAFTRTNA
LILRNPSAFNFLDACGLSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
>Mature_457_residues
TTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAAGTVLSPLAGIPVSV
KDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGNPRSPYHRDVPGDARISG
GSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLAG
LEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFPALDRLPEINRFGFSPIEAYAWHRPL
LATHRDQYDPRVLTRILKGEPASAADYLDLLVARAAMLDEAAHTVWSRFDALVAPTVPVVPPRIAELETDDDAFTRTNAL
ILRNPSAFNFLDACGLSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=316, Percent_Identity=31.3291139240506, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI195972892, Length=308, Percent_Identity=28.2467532467532, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=39.8550724637681, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=25.858123569794, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=29.5031055900621, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=351, Percent_Identity=27.9202279202279, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=35.8823529411765, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=161, Percent_Identity=36.6459627329193, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=321, Percent_Identity=27.4143302180685, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=29.1989664082687, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=434, Percent_Identity=25.5760368663594, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=169, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648113, Length=305, Percent_Identity=32.4590163934426, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI45550774, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648435, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=37.6712328767123, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=37.6712328767123, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356731, Length=207, Percent_Identity=33.8164251207729, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=24.3407707910751, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI161078093, Length=318, Percent_Identity=26.1006289308176, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24652985, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922090, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652981, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652983, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47547; Mature: 47415

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGNPRSPYHRDVPGDARISGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFPALDR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
LPEINRFGFSPIEAYAWHRPLLATHRDQYDPRVLTRILKGEPASAADYLDLLVARAAMLD
CCCHHHCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWSRFDALVAPTVPVVPPRIAELETDDDAFTRTNALILRNPSAFNFLDACGLSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHCCEEEEECCCHHHHHHHCCCCCC
CHPRDAAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAAGTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGNPRSPYHRDVPGDARISGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDTALKHLEAAGAIVTEVRFPALDR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
LPEINRFGFSPIEAYAWHRPLLATHRDQYDPRVLTRILKGEPASAADYLDLLVARAAMLD
CCCHHHCCCCHHHHHHHCCCHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWSRFDALVAPTVPVVPPRIAELETDDDAFTRTNALILRNPSAFNFLDACGLSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCHHHHCCEEEEECCCHHHHHHHCCCCCC
CHPRDAAPVGLMLAAAPHRDDALLAIGQSVEAVLNTIR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA