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The map label for this gene is spoT [H]

Identifier: 116689024

GI number: 116689024

Start: 1106751

End: 1109117

Strand: Direct

Name: spoT [H]

Synonym: Bcen2424_1001

Alternate gene names: 116689024

Gene position: 1106751-1109117 (Clockwise)

Preceding gene: 116689023

Following gene: 116689025

Centisome position: 31.77

GC content: 65.1

Gene sequence:

>2367_bases
ATGAGCACCACCCCATCGTCCGCCTCCGCGGATTCGACCACCGAAGCCACGGCCCAGTCGCCTGCGCGCCAGTACATCGA
CGCGGTCCTCGAACAGTCCTTCCGGCATCTGTTCGGGCCGACCGCCACACCGGAGCAGCCGCGCAAGCACGGCGTCGTTT
CGATCGCGAAACTGACCGCCGCGCTTGCCGAGTATCTCGCTCCGGACGAAATCAAAGAGGTCAAGGCGGCGTTCCACTTC
AGCGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAGCCCTACATCACCCATCCCGTCGCCGTCGCGGAAATCTG
CGCCGGCTGGAAGCTCGACGCGCAGGCCGTGATGGCCGCGTTGCTGCACGACGTGATGGAAGACCAGGGCGTGACCAAGA
ACGAGCTGGCCGAGCGGTTCGGCCCGAAGGTCGCCGAGCTGGTCGACGGCCTGTCGAAGCTCGACAAGATGGAATTCCGC
AGCCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCGCGCGACGTGCGCGTCATTCTCGTCAA
GCTCGCCGATCGCTTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAAAAACGCCGCCGCGTCGCGCGGGAAACGC
TCGACATCTACGCGCCGATCGCGCACCGGCTCGGGTTGAACAACACGTATCGCGAGCTGCAGGACATGAGCTTCGCGAAC
TTCAACCCGCATCGCTACGCGACGCTCGAGAAGGCGGTGAAGGCCGCGCGCGGCAATCGCCGCGAAGTGATCAGCAAGAT
CCTCGAGGCCGCGCAGCGCGCGATGGCCGACGCGAAGATCGATGCCGAGATCACCGGCCGCGAGAAAACCATCTACAGCG
TCTATCGCAAGATGCGCGACAAGCAGCTGTCGTTCTCGCAGGTGCTCGACGTGTACGGCTTCCGCGTCGTCGTCGACAGC
CCGCTCGACTGCTACACGTGCATCGGCGCGCTGCACGCGCTGTACAAGCCCGTGCCCGGCAAGTTCAAGGATTACATCGC
GATCCCGAAGATCAACGGCTATCAGTCGTTGCACACGACGCTCGTCGGCCCGTTCGGCGCGCCGATCGAGTTCCAGGTGC
GTACGCGCAAGATGCACGAGATCGCCGAGGCGGGGGTCGCCGCGCACTGGCTGTACAAGAACGGCAGCGCGGACCTCAGC
GACGTGCAGAAGCGCGCGCATCAGTGGCTGAAGTCGCTGCTCGACATCCAGAGCGAAGCGGGCGATTCGAGCGAATTCCT
CGAGCACGTGAAGATCGACCTGTTCCCCGACGCGGTCTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGCGTG
GCGCGACGGCGCTCGATTTCGCGTATTCGATCCACAGCGATCTCGGCAACCAGTGCGTGGCCGTGAAGATCAACAACGAA
CTGCTGCCGCTGCGCACCGAATTGAAGAGCGGCGACATCGTCGAGGTGATCACCGCGCCGTATTCGAAGCCGAACCCTGC
ATGGCTCGGCTTCGTGCGCACCGGCAAGGCACGCTCGGCGATCCGTCACTACCTGAAGACGATGCGCCTGAACGAGTCCG
TGCAGCTGGGCGAGCGGCTCGTTGACCAGAGCCTGAAGGGCTATGGTCTCGCGCTGGCCGACGTCGAGCCGGAAGTGTGG
GAGAAGCTCGTGCAGTGGACGGGCAACAAGAGCCGTCAGGAAATCTTCGCGGACATCGGCCTCGGTCGTCGCGTCGCCGC
GGTGATGGCCAAGCGCATCGAGGTGCTGATGAGCGGCCGCGACGCGGACGACGATCTGCCGAAGTCCGAGCGGCATCCCG
CGCATCATGCGCCGCCGGTCGTGATTACCGGCACCGAAGGGATGTCCGTGCAGTTGTCCGCCTGCTGCCGGCCGATCCCG
GGCGACGCGATCATGGGCTACATCGGCATCGGTCTGGGCATGGCGATTCATACGACGGACTGCCGTGTCGCGCAGCGCAT
CCATCGCCGCGATCCGGGCCGCTGGATCGACGTCGAATGGGCGCCGCAGCCGGGCCGCCTGTTCGATGTCGCGGTGAAGG
CGCTGGTGAAGAACACGAAGGGCATCTTCGCGCGCGTCGCGGCGGACATCACGTCGGCCGACGCGAACATCGTTCATATC
GCGATGGACGAGGATCTGACGCACGAATCGACGGTGCTGCGCTTCGTGATCCAGGTCAGCGACCGCGTGCATCTCGCGAA
CGTGATGCGGCGCGTGAGAACCAATCCCGACGTGATGCGGATCATGCGCGAGCGCTCGACCGACGACGGTGCGCATGCGC
GCCATGACGGCGGCATGCGTATCGAGCGCGAGCGGCAGGACTACTGA

Upstream 100 bases:

>100_bases
GGTCGGCGTCGAGATGCTGAAGAAGGTGCCGGTGTAACGCGCATCCGGCCCGTTCCAGCCATGTAGTCCACGCGCGCAAC
GACTCGACCTGGAGGCGAAT

Downstream 100 bases:

>100_bases
CGCTCACGTGCCCTGGCGGGTGGATTCGATGCTCGACGGACGGCGGCCAATGGCCGCCGTTTTCATTGACGCCGACGAAA
GGGTGCGCGACGTTGGCGGC

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLAPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKNELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLS
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY

Sequences:

>Translated_788_residues
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLAPDEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKNELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLS
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY
>Mature_787_residues
STTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLAPDEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKNELAERFGPKVAELVDGLSKLDKMEFRS
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFANF
NPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDSP
LDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLSD
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVWE
KLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPG
DAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHIA
MDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=45.4929577464789, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.9180087847731, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87984; Mature: 87853

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLAPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKNELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLSDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVEPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC
>Mature Secondary Structure 
STTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLAPDEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKNELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGSADLSDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVEPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]