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The map label for this gene is trmB

Identifier: 116688941

GI number: 116688941

Start: 1026430

End: 1027197

Strand: Reverse

Name: trmB

Synonym: Bcen2424_0918

Alternate gene names: 116688941

Gene position: 1027197-1026430 (Counterclockwise)

Preceding gene: 116688943

Following gene: 116688937

Centisome position: 29.48

GC content: 67.97

Gene sequence:

>768_bases
ATGATGCACGACGATCCGAACGAAGCCGGCCTGCCGCCGCACAACGACGCCATTCCTGACGAAACTGCCGAAGGCGCCGA
CGAAGTCAACCCGCTGCACCACCGCCGCATCCGCAGCTTCGTGACGCGTGCCGGCCGCGTGTCGACCGGCCAGCGCCGCG
CACTCGACGAATTCGGCCCGCGCTTCGTGGTCCCGTATGCGCCGGAGATGCCCGACTGGGACGCGATCTTCGGCCGCAGC
GCACCGCGCATCCTCGAGATCGGTTTCGGGATGGGCGCGTCGACCGCGGAAATCGCCGCGCACCGCCCGAGTGACGATTT
CCTCGGCGTCGAGGTGCACGAGCCGGGCGTCGGCGCGCTGCTGAAGCTGATCGGCGAGCAGGGTCTGTCGAACATCCGCA
TCATCCAGCACGACGCGGTCGAGGTGCTCGAGCACATGATCGCGCCGGCAAGCCTCGACGGCGTGCACATCTTCTTCCCC
GATCCGTGGCACAAGGCGCGTCACCACAAGCGCCGGCTGATCCAGCCGCCGCTCGTCGCGCATCTCGCGTCGCGCCTGAA
GCCCGGCGCGTACCTGCACTGCGCGACCGACTGGCAGAACTACGCGGAACAGATGCTGGAAGTGCTCGGCGCGGAACCGA
CGCTCGAAAACACCGCTGCCGATTACGCACCGCGCCCGGACTACCGCCCGGTGACGAAATTCGAACGGCGCGGGTTGCGA
CTCGGCCACGGCGTGTGGGACCTGGTGTTCCGCAAGCGAGCAGACTAA

Upstream 100 bases:

>100_bases
CCGCCTCGTCGAAGGCGGCTTTTTTGTACCATGCGCCGAATCGCCCGGCAGCACTGCTGCGCAACAGGTCCTCGCCCGAA
CCGAGCCTTTCTCTGTACCG

Downstream 100 bases:

>100_bases
GCGCGCCCCCGCCCCGCGCAGAATAAAGAAAGCCCGACCGGCATTTCACGCCGGTCGGGCTTTTTTCATAAAGCGGCGCA
TCGGCCCCTCGCCGGCTTCA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MMHDDPNEAGLPPHNDAIPDETAEGADEVNPLHHRRIRSFVTRAGRVSTGQRRALDEFGPRFVVPYAPEMPDWDAIFGRS
APRILEIGFGMGASTAEIAAHRPSDDFLGVEVHEPGVGALLKLIGEQGLSNIRIIQHDAVEVLEHMIAPASLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLASRLKPGAYLHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAD

Sequences:

>Translated_255_residues
MMHDDPNEAGLPPHNDAIPDETAEGADEVNPLHHRRIRSFVTRAGRVSTGQRRALDEFGPRFVVPYAPEMPDWDAIFGRS
APRILEIGFGMGASTAEIAAHRPSDDFLGVEVHEPGVGALLKLIGEQGLSNIRIIQHDAVEVLEHMIAPASLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLASRLKPGAYLHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAD
>Mature_255_residues
MMHDDPNEAGLPPHNDAIPDETAEGADEVNPLHHRRIRSFVTRAGRVSTGQRRALDEFGPRFVVPYAPEMPDWDAIFGRS
APRILEIGFGMGASTAEIAAHRPSDDFLGVEVHEPGVGALLKLIGEQGLSNIRIIQHDAVEVLEHMIAPASLDGVHIFFP
DPWHKARHHKRRLIQPPLVAHLASRLKPGAYLHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
LGHGVWDLVFRKRAD

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=240, Percent_Identity=54.5833333333333, Blast_Score=251, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6320000, Length=176, Percent_Identity=26.1363636363636, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BURCA (Q1BYF4)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_620324.1
- ProteinModelPortal:   Q1BYF4
- SMR:   Q1BYF4
- STRING:   Q1BYF4
- GeneID:   4090931
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_0439
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   BCEN331271:BCEN_0439-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28529; Mature: 28529

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: BINDING 86-86 BINDING 111-111 BINDING 138-138 BINDING 161-161 BINDING 165-165 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHDDPNEAGLPPHNDAIPDETAEGADEVNPLHHRRIRSFVTRAGRVSTGQRRALDEFGP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RFVVPYAPEMPDWDAIFGRSAPRILEIGFGMGASTAEIAAHRPSDDFLGVEVHEPGVGAL
EEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHH
LKLIGEQGLSNIRIIQHDAVEVLEHMIAPASLDGVHIFFPDPWHKARHHKRRLIQPPLVA
HHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHH
HLASRLKPGAYLHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
HHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCC
LGHGVWDLVFRKRAD
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MMHDDPNEAGLPPHNDAIPDETAEGADEVNPLHHRRIRSFVTRAGRVSTGQRRALDEFGP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
RFVVPYAPEMPDWDAIFGRSAPRILEIGFGMGASTAEIAAHRPSDDFLGVEVHEPGVGAL
EEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHH
LKLIGEQGLSNIRIIQHDAVEVLEHMIAPASLDGVHIFFPDPWHKARHHKRRLIQPPLVA
HHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHH
HLASRLKPGAYLHCATDWQNYAEQMLEVLGAEPTLENTAADYAPRPDYRPVTKFERRGLR
HHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHHCCCC
LGHGVWDLVFRKRAD
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA