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The map label for this gene is rfbE [H]

Identifier: 116688900

GI number: 116688900

Start: 980055

End: 980807

Strand: Direct

Name: rfbE [H]

Synonym: Bcen2424_0877

Alternate gene names: 116688900

Gene position: 980055-980807 (Clockwise)

Preceding gene: 116688899

Following gene: 116688901

Centisome position: 28.13

GC content: 61.62

Gene sequence:

>753_bases
ATGGCACACATTGAACTGAAGAACGTGACGCTGGATTTGCCGATCTACGACGTCCAGGGCCGCTCGCTGAAGAAACAGGT
GCTTCGCATGGGGCGACGCAACCGGATCGCGGAGGGTAACGACGGCGTCATCGTCGTACGGGCGCTCGACGATCTGAGTT
TCCGCTTCGATCGAGGCGATCGCGTGGGGCTCATTGGTCACAACGGCGCGGGAAAGTCGACGCTGCTGCGCGCGATGGCC
GGCATTTATCCTCCGACAGCCGGCTCGCTGTCACGAGTGGGCAAGGCCGTGCCGCTGCTCGACATCAGCCTCGGCATGGA
CGAGAACTCGACCGGTATGCAGAACATCCGGTTGCGCGGCCTGCTGCTCGGCATGACTGACGCGGAGATTCGCAAAAAGC
AGAACGATATCGCTGAGTTCAGCGAATTGGGCGACTACCTCGATCTGCCGATCCGTACCTATTCGAGCGGCATGAAGATG
CGGCTTGCATTCGCCGTTTCCACGGCGGTCGACGCCGAGATCCTGCTGCTCGATGAAGTGATGGGCGTGGGCGATGCGTC
GTTCATGCACAAGGCCGAGGCGCGTCTCGAGGAACTGCACGACCGCGCCGAGATCGTTGTGCTCGCGATGCACTCGAACA
AGGAGATCCGCAAGGTGTGCAACAAGGCGCTGTGGATGGAGCGCGGGCGTGTGCGCGCTTTCGGGGAAGTCGACGACGTC
GTGTCGCAATATGCTGCTTCGGTGGGGGAGTGA

Upstream 100 bases:

>100_bases
GGAGTCACCATCGGCATGGCGGTCATTGGATGGCCGATCGCACTGCTCATGACCGGTCGTTATCTGAAACGTCTTCCGTA
CTGGGTCTGAAGGGGGCGAC

Downstream 100 bases:

>100_bases
TGGTTGTGAAGTCGAACGCCGGTATGGCTGGGTGCTGAACCGATGAAGTTGCGCAAAGCACGCTCGTTCGTGAACCGGGT
GGCGGGTGACGGTGGATTGC

Product: ABC transporter related

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MAHIELKNVTLDLPIYDVQGRSLKKQVLRMGRRNRIAEGNDGVIVVRALDDLSFRFDRGDRVGLIGHNGAGKSTLLRAMA
GIYPPTAGSLSRVGKAVPLLDISLGMDENSTGMQNIRLRGLLLGMTDAEIRKKQNDIAEFSELGDYLDLPIRTYSSGMKM
RLAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLEELHDRAEIVVLAMHSNKEIRKVCNKALWMERGRVRAFGEVDDV
VSQYAASVGE

Sequences:

>Translated_250_residues
MAHIELKNVTLDLPIYDVQGRSLKKQVLRMGRRNRIAEGNDGVIVVRALDDLSFRFDRGDRVGLIGHNGAGKSTLLRAMA
GIYPPTAGSLSRVGKAVPLLDISLGMDENSTGMQNIRLRGLLLGMTDAEIRKKQNDIAEFSELGDYLDLPIRTYSSGMKM
RLAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLEELHDRAEIVVLAMHSNKEIRKVCNKALWMERGRVRAFGEVDDV
VSQYAASVGE
>Mature_249_residues
AHIELKNVTLDLPIYDVQGRSLKKQVLRMGRRNRIAEGNDGVIVVRALDDLSFRFDRGDRVGLIGHNGAGKSTLLRAMAG
IYPPTAGSLSRVGKAVPLLDISLGMDENSTGMQNIRLRGLLLGMTDAEIRKKQNDIAEFSELGDYLDLPIRTYSSGMKMR
LAFAVSTAVDAEILLLDEVMGVGDASFMHKAEARLEELHDRAEIVVLAMHSNKEIRKVCNKALWMERGRVRAFGEVDDVV
SQYAASVGE

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=206, Percent_Identity=27.1844660194175, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI6005701, Length=222, Percent_Identity=27.027027027027, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI27262624, Length=207, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27262626, Length=207, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI27436953, Length=196, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI153792144, Length=212, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI105990541, Length=234, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI150417984, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI31657092, Length=214, Percent_Identity=27.1028037383178, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI47078218, Length=204, Percent_Identity=25.9803921568627, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI45446740, Length=204, Percent_Identity=25.9803921568627, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87081791, Length=215, Percent_Identity=26.9767441860465, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994943, Length=213, Percent_Identity=26.7605633802817, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=28.7037037037037, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081709, Length=209, Percent_Identity=24.4019138755981, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790467, Length=198, Percent_Identity=26.2626262626263, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081782, Length=209, Percent_Identity=26.3157894736842, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786398, Length=227, Percent_Identity=22.4669603524229, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=241, Percent_Identity=24.4813278008299, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=180, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007184, Length=186, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221500365, Length=186, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24666092, Length=209, Percent_Identity=27.7511961722488, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648314, Length=205, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27555; Mature: 27424

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIELKNVTLDLPIYDVQGRSLKKQVLRMGRRNRIAEGNDGVIVVRALDDLSFRFDRGD
CCEEEEECEEEECCEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCC
RVGLIGHNGAGKSTLLRAMAGIYPPTAGSLSRVGKAVPLLDISLGMDENSTGMQNIRLRG
EEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCHHEEEEE
LLLGMTDAEIRKKQNDIAEFSELGDYLDLPIRTYSSGMKMRLAFAVSTAVDAEILLLDEV
EEEECCHHHHHHHHHHHHHHHHHCHHHCCCHHHHCCCCEEEEEEEEHHHCCHHHEEHHHH
MGVGDASFMHKAEARLEELHDRAEIVVLAMHSNKEIRKVCNKALWMERGRVRAFGEVDDV
HCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCHHHHH
VSQYAASVGE
HHHHHHHCCC
>Mature Secondary Structure 
AHIELKNVTLDLPIYDVQGRSLKKQVLRMGRRNRIAEGNDGVIVVRALDDLSFRFDRGD
CEEEEECEEEECCEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCC
RVGLIGHNGAGKSTLLRAMAGIYPPTAGSLSRVGKAVPLLDISLGMDENSTGMQNIRLRG
EEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCHHEEEEE
LLLGMTDAEIRKKQNDIAEFSELGDYLDLPIRTYSSGMKMRLAFAVSTAVDAEILLLDEV
EEEECCHHHHHHHHHHHHHHHHHCHHHCCCHHHHCCCCEEEEEEEEHHHCCHHHEEHHHH
MGVGDASFMHKAEARLEELHDRAEIVVLAMHSNKEIRKVCNKALWMERGRVRAFGEVDDV
HCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCHHHHH
VSQYAASVGE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]