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The map label for this gene is mhpA [H]

Identifier: 116688840

GI number: 116688840

Start: 916248

End: 917909

Strand: Direct

Name: mhpA [H]

Synonym: Bcen2424_0817

Alternate gene names: 116688840

Gene position: 916248-917909 (Clockwise)

Preceding gene: 116688838

Following gene: 116688841

Centisome position: 26.3

GC content: 70.1

Gene sequence:

>1662_bases
ATGGCGACCATGAGCATCGATTACCAGACGCTGAAGTTCGAGTACCGCGCCCACGCGCACGACGCGGCCGTCCACCCGGT
GATCGTCGTCGGCGCGGGCCCGGTGGGCCTGGCCGCCGCGATCGACCTCGCGCAGCAGGGCGTGCCGGTCGTGCTGCTCG
ACGACGACGACACGCTGTCGACCGGCTCGCGCGCGATCTGCTTCGCGAAGCGCACGCTCGAGATCTTCGACCGGCTCGGC
TGCGGCGAGCGCTTCGTCGACAAGGGCGTGAGCTGGCACGTCGGCAAGGTGTTCCTGCAGGACGAGCAGCTTTACGCGTT
CGACCTGCTGCCCGAGGAAGGGCATGCGCGTCCCGCGTTCATCAACCTGCAGCAGTACTACGTCGAAGGCTACCTGGCCG
ACCGCGCGTTCGAGCTGCCGAACATCGACATCCGCTGGAAGCACAAGGTGACGGGCGTCGACCAGTCGGCCGAACACGCG
GTGCTGACGATCGAGACGCCGGACGGCGTCGCGACGCTGCGCGCGCAATACGTGATCGCGGCCGACGGCTCGCGCAGCCC
GATGCGCGCGGCGATGGGCCTGGAAAGCCGCGGCCGCACGTTCAAGGACCGCTTCCTGATCGCCGACGTGAAGATGAAGG
CGGAGTTTCCGACCGAGCGCTGGTTCTGGTTCGACCCGCCGTTTCACCGCAACCAGTCGGTGCTGCTGCATCGTCAGCCG
GACAACGTGTGGCGCATCGACTTCCAGCTCGGCTGGGATGCCGATCCGATCGCCGAGAAGCAGCCGGAGCGCGTGATTCC
ACGTGTGCGCGCGCTGCTCGGCGCGGACGTCGAGTTCGAGCTCGAATGGGTGAGCGTCTATACGTTCCGCTGCCAGCGGA
TGGACACGTTCCGTCATGGCCGCGTGCTGTTCGCGGGCGATTCGGCGCACGGCGTGTCGCCGTTCGGCGCGCGCGGTGCG
AACAGCGGCGTGCAGGACGCGGACAACCTCGCGTGGAAGCTGAAACTGGTGCTCGACGGCCGTGCGCACGACCGCCTGCT
CGACACCTATGCGAGCGAGCGGGAGTTCGCGGCGGACGAGAACATCCGCAACTCGACGCGCTCGACCGATTTCATCACGC
CGAAAAGCGCGGTGTCGCGCGTGTTCCGCGACGCGACGCTGAAGCTCGCGCGCGACTGCGAATTTGCGCGCAAGCTGGTG
AACAGCGGGCGCCTGTCGGTGCCGGCCGTGCTGGCCGATTCGCCGCTCAACACTCCGGACCGCGGCGGCGACGCATTCGC
ATGCGCGATGCGGCCGGGCGCGGCGGCGGCCGATGCACCGGTGCGCGCGCAGGGTGCGTCGGGCTGGCTGTTGCAGCATC
TGCGCGGCGGCTTCACCGGTGTGCTGTTCGGATTGCCGGGCGACGCGGCCGCGCTCGCGCAGGCCGTCGACGGCCTCGCG
CCGGCGGTGCGGCCGGTGCTCGTCGTGCCGGCCGGGCACGCGCAGCCGGCGGCCGGCGTCGACGTCGTGGAGGACATCGA
CGGGCTCGCCGCGCAGCGCTACGACGCGCAGCCCGGCACGTTTTACCTGCTGCGTCCCGACCAGCACGTCTGCGCCCGCA
CGCGCACGCTCGACCGTCAGGCGATTGCCGACGCACTGGCGCGCGCGAGCTGCGCACGCTGA

Upstream 100 bases:

>100_bases
GACGTGGCGGTTATTGACAGGACAAGCGGGCAGGCGCGCCGCGAGGAGACGGACGGAGCGACGGGCGCGGCGGTGCGCGC
ACGGCGGGGTTGACGCGCGG

Downstream 100 bases:

>100_bases
ATACGATAAAGACACCGGAGACACCGTCATGCCCCCACTCGATACCCGCCCGCGCCTGGCCGACCCCGATGCGTTCTACG
AAGCGCTGATCGACATGCAT

Product: FAD-dependent oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MATMSIDYQTLKFEYRAHAHDAAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLG
CGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGVDQSAEHA
VLTIETPDGVATLRAQYVIAADGSRSPMRAAMGLESRGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHRNQSVLLHRQP
DNVWRIDFQLGWDADPIAEKQPERVIPRVRALLGADVEFELEWVSVYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGARGA
NSGVQDADNLAWKLKLVLDGRAHDRLLDTYASEREFAADENIRNSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKLV
NSGRLSVPAVLADSPLNTPDRGGDAFACAMRPGAAAADAPVRAQGASGWLLQHLRGGFTGVLFGLPGDAAALAQAVDGLA
PAVRPVLVVPAGHAQPAAGVDVVEDIDGLAAQRYDAQPGTFYLLRPDQHVCARTRTLDRQAIADALARASCAR

Sequences:

>Translated_553_residues
MATMSIDYQTLKFEYRAHAHDAAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLG
CGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGVDQSAEHA
VLTIETPDGVATLRAQYVIAADGSRSPMRAAMGLESRGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHRNQSVLLHRQP
DNVWRIDFQLGWDADPIAEKQPERVIPRVRALLGADVEFELEWVSVYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGARGA
NSGVQDADNLAWKLKLVLDGRAHDRLLDTYASEREFAADENIRNSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKLV
NSGRLSVPAVLADSPLNTPDRGGDAFACAMRPGAAAADAPVRAQGASGWLLQHLRGGFTGVLFGLPGDAAALAQAVDGLA
PAVRPVLVVPAGHAQPAAGVDVVEDIDGLAAQRYDAQPGTFYLLRPDQHVCARTRTLDRQAIADALARASCAR
>Mature_552_residues
ATMSIDYQTLKFEYRAHAHDAAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLSTGSRAICFAKRTLEIFDRLGC
GERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAFINLQQYYVEGYLADRAFELPNIDIRWKHKVTGVDQSAEHAV
LTIETPDGVATLRAQYVIAADGSRSPMRAAMGLESRGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHRNQSVLLHRQPD
NVWRIDFQLGWDADPIAEKQPERVIPRVRALLGADVEFELEWVSVYTFRCQRMDTFRHGRVLFAGDSAHGVSPFGARGAN
SGVQDADNLAWKLKLVLDGRAHDRLLDTYASEREFAADENIRNSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKLVN
SGRLSVPAVLADSPLNTPDRGGDAFACAMRPGAAAADAPVRAQGASGWLLQHLRGGFTGVLFGLPGDAAALAQAVDGLAP
AVRPVLVVPAGHAQPAAGVDVVEDIDGLAAQRYDAQPGTFYLLRPDQHVCARTRTLDRQAIADALARASCAR

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=365, Percent_Identity=27.6712328767123, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60705; Mature: 60574

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMSIDYQTLKFEYRAHAHDAAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLS
CCEEEECEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCC
TGSRAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCEE
INLQQYYVEGYLADRAFELPNIDIRWKHKVTGVDQSAEHAVLTIETPDGVATLRAQYVIA
EEHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEE
ADGSRSPMRAAMGLESRGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHRNQSVLLHRQP
ECCCCCCHHHHHCCCCCCCEECCEEEEEEEEEEECCCCCCEEEECCCCCCCCEEEEEECC
DNVWRIDFQLGWDADPIAEKQPERVIPRVRALLGADVEFELEWVSVYTFRCQRMDTFRHG
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHCCC
RVLFAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAHDRLLDTYASEREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHCCCC
NIRNSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKLVNSGRLSVPAVLADSPLNTPD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEECCCCCCCC
RGGDAFACAMRPGAAAADAPVRAQGASGWLLQHLRGGFTGVLFGLPGDAAALAQAVDGLA
CCCCEEEEEECCCCCCCCCCCEECCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
PAVRPVLVVPAGHAQPAAGVDVVEDIDGLAAQRYDAQPGTFYLLRPDQHVCARTRTLDRQ
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
AIADALARASCAR
HHHHHHHHHHCCC
>Mature Secondary Structure 
ATMSIDYQTLKFEYRAHAHDAAVHPVIVVGAGPVGLAAAIDLAQQGVPVVLLDDDDTLS
CEEEECEEEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCC
TGSRAICFAKRTLEIFDRLGCGERFVDKGVSWHVGKVFLQDEQLYAFDLLPEEGHARPAF
CCCEEEEEHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEEECCEEEEEEECCCCCCCCCEE
INLQQYYVEGYLADRAFELPNIDIRWKHKVTGVDQSAEHAVLTIETPDGVATLRAQYVIA
EEHHHHHHHHHHHCCEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEE
ADGSRSPMRAAMGLESRGRTFKDRFLIADVKMKAEFPTERWFWFDPPFHRNQSVLLHRQP
ECCCCCCHHHHHCCCCCCCEECCEEEEEEEEEEECCCCCCEEEECCCCCCCCEEEEEECC
DNVWRIDFQLGWDADPIAEKQPERVIPRVRALLGADVEFELEWVSVYTFRCQRMDTFRHG
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHCCC
RVLFAGDSAHGVSPFGARGANSGVQDADNLAWKLKLVLDGRAHDRLLDTYASEREFAADE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHCCCC
NIRNSTRSTDFITPKSAVSRVFRDATLKLARDCEFARKLVNSGRLSVPAVLADSPLNTPD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEECCCCCCCC
RGGDAFACAMRPGAAAADAPVRAQGASGWLLQHLRGGFTGVLFGLPGDAAALAQAVDGLA
CCCCEEEEEECCCCCCCCCCCEECCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHH
PAVRPVLVVPAGHAQPAAGVDVVEDIDGLAAQRYDAQPGTFYLLRPDQHVCARTRTLDRQ
HCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHH
AIADALARASCAR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA