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The map label for this gene is tgt [H]

Identifier: 116688745

GI number: 116688745

Start: 803089

End: 804276

Strand: Reverse

Name: tgt [H]

Synonym: Bcen2424_0722

Alternate gene names: 116688745

Gene position: 804276-803089 (Counterclockwise)

Preceding gene: 116688746

Following gene: 116688744

Centisome position: 23.09

GC content: 65.49

Gene sequence:

>1188_bases
ATGACCGAAGGTTCATCCCACGATACGCACGCCCCCGTGCAGGACCGCCCGGCCAACGGGCTCGAATTCGAATTGCTGAC
GACCGACGGCCACGCGCGCCGCGGCCGCGTGAAGCTCAATCACGGCACGGTCGAGACACCGATCTTCATGCCGGTCGGCA
CGTACGGCACCGTGAAGGCGATCCAGCCGCGCGAGCTGCACGAGATCAAGGCCCAGATCATCCTCGGCAACACGTTCCAC
CTGTGGCTGCGCCCGGGCCTCGACACGATCGACGCGCACGGCGGCCTGCACGGCTTCATGGGCTGGAACAAGCCGATCCT
GACCGATTCGGGTGGCTTCCAGGTGTTCTCGCTCGGCGACCTGCGCAAGATCACCGAGGACGGCGTCACGTTCGCGTCGC
CGATCAACGGCGACAAGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGGTGCTGAACTCCGACGTCGTGATG
CAGTTCGACGAGTGCACGCCGTACGCGACCAACGGCGTGCCGACGACGCACCAGGAAGCGGCCGACTCGATGCGCATGTC
GCTGCGCTGGGCGAAGCGCTCGCTCGACGAATTCCACCGGCTCGGCAACCCGAATGCGCTGTTCGGGATCGTCCAGGGCG
GGATGTTCGAGGACCTGCGCGACGAATCGCTCGCGGGCCTCGCGGAACTCGGCTTCCACGGCCTCGCGATCGGCGGGCTG
TCGGTGGGCGAGCCGAAGGAAGACATGATGCGCGTGCTGCAGCACGTGGGCCCGAAGCTGCCGGCCAACAAGCCGCACTA
CCTGATGGGCGTGGGCACGCCGGAGGATCTGGTCGAGGGCGTCGCGAACGGCATCGACATGTTCGACTGCGTGATGCCGA
CCCGCAACGCGCGCAACGGGTGGCTCTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAACTCGTTG
AAGCCGCTCGATTCGACCTGCACCTGCTACACGTGCCAGAACTTCTCGCGCGGCTACCTGCATCACCTGCACCGGGTCGG
CGAAATCCTCGGCGCGCAGCTGAACACGATCCACAACCTGCACTACTACCTGCAGCTGATGAGCGAGATCCGCGACGCGA
TCGAAACGCATACGTTCGACGCATTCCGGCAGCGCTTCGCGGAGGATCGCGCCAGAGGGGTCGACTGA

Upstream 100 bases:

>100_bases
CGCTACGCTGGCGCGCACCCGCTTTTCCCCGCCGCCGATTTCCCGGGCGGCGTTTCCAACATGCGCCGGACTGTTTTCCG
GTAGCAAGGAGAGCAACACG

Downstream 100 bases:

>100_bases
CGGGCCGGCCGGCGGCCCGCACCGCGGCCAGTCACATAGGACGCGTACTAATTGGCACACTCAAATCGCCAGTCGCGACC
GCCAAACATTACAATTACCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFH
LWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVM
QFDECTPYATNGVPTTHQEAADSMRMSLRWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
SVGEPKEDMMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSL
KPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD

Sequences:

>Translated_395_residues
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFH
LWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVM
QFDECTPYATNGVPTTHQEAADSMRMSLRWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
SVGEPKEDMMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSL
KPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD
>Mature_394_residues
TEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFHL
WLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQ
FDECTPYATNGVPTTHQEAADSMRMSLRWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLS
VGEPKEDMMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=44.3820224719101, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=64.1711229946524, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=42.0485175202156, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=29.2682926829268, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=44.5070422535211, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI21355311, Length=166, Percent_Identity=29.5180722891566, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43940; Mature: 43808

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCEEC
IQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGD
CCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEEECCH
LRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEA
HHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
ADSMRMSLRWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCC
SVGEPKEDMMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNG
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCC
WLFTRFGDVKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNL
EEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGTVETPIFMPVGTYGTVKA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCEEEECCCCCCEEC
IQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHGFMGWNKPILTDSGGFQVFSLGD
CCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCEEECCCCEEEEECCH
LRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHQEA
HHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
ADSMRMSLRWAKRSLDEFHRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCC
SVGEPKEDMMRVLQHVGPKLPANKPHYLMGVGTPEDLVEGVANGIDMFDCVMPTRNARNG
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCC
WLFTRFGDVKIRNATHKNSLKPLDSTCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNL
EEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HYYLQLMSEIRDAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA