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The map label for this gene is purH [H]

Identifier: 116688714

GI number: 116688714

Start: 769630

End: 771195

Strand: Reverse

Name: purH [H]

Synonym: Bcen2424_0691

Alternate gene names: 116688714

Gene position: 771195-769630 (Counterclockwise)

Preceding gene: 116688715

Following gene: 116688713

Centisome position: 22.14

GC content: 66.67

Gene sequence:

>1566_bases
ATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAGACCGGCATCGTCGACTTCGCGAAGTCGCTGTCCGACCTCGGCGT
CAAGCTGCTGTCGACGGGCGGCACCGCGAAACTCCTCGCCGACGCCGGCCTGCCCGTGACCGAAGTGGCCGATTACACGG
GCTTCCCGGAAATGCTCGATGGGCGCGTGAAGACGCTCCACCCGAAGGTGCACGGCGGCATCCTCGCCCGCCGCGACCTG
CCCGAGCACATGCAGGCGCTCGAACAGCACGGCATCCCGACGATCGACCTGCTCGTCGTGAACCTGTATCCGTTCGTCGC
GACGATCGCGAAGGACGACTGCACGCTCGCCGATGCGATCGAGAACATCGACATCGGCGGCCCGACGATGCTGCGCTCGG
CCGCGAAGAACCACCGCGACGTGACGGTCGTCGTCGATCCGGCCGACTACGCGGTCGTGCTCGACGAAATGAAGGCGAAC
GGCAACACGGTCGGCTACCCGACCAACTTCCGCCTCGCGACGAAGGTGTTCGCGCACACCGCGCAATACGACGGCGCGAT
CACGAACTACCTGACGAGCCTGACCGACGAGCTGAAGCACGCATCGCGCAGCACGTACCCGGCCACGCTGAACCTGGCGT
TCGACAAGGTGCAGGACCTGCGCTACGGCGAGAACCCGCACCAGAGCGCGGCGTTCTACCGCGACCTCGCGACGCCGGCC
GGCGCGCTGGCGAACTACCGCCAGCTGCAGGGCAAGGAACTGTCGTACAACAACATCGCGGATTCGGACGCGGCGTGGGA
ATGCGTGAAGACGTTCGACGCGCCGGCCTGCGTGATCATCAAGCATGCGAACCCGTGTGGTGTCGCGGTGGGCAACGACT
CGGCCGACGCATACGCGAAGGCGTTCCAGACCGATCCGACCTCGGCATTCGGCGGCATCATCGCGTTCAACCGCGAAGTC
GACGAAGCGGCCGCGCAGGCGGTGGCGAAGCAGTTCGTCGAAGTGCTGATCGCCCCGTCGTTCTCCGACGCCGCGAAGCA
GGTGTTCGCCGCGAAGCAGAACGTGCGCCTGCTCGAGATTGCACTGGGTGACGGCCACAACGCATTCGACCTGAAGCGCG
TCGGCGGCGGCCTGCTCGTGCAATCGCTCGATTCGCGCAACGTGCAGCCGAGCGAACTGCGCGTCGTCACGAAGCGCCAG
CCGACCGCGAAGGAAATGGACGACCTGCTGTTCGCGTGGCGCGTCGCGAAATACGTGAAGTCGAACGCGATCGTGTTCTG
CGGCAACGGCATGACGCTCGGCGTCGGCGCCGGCCAGATGAGCCGCGTCGATTCCGCGCGCATCGCGAGCATCAAGGCAC
AGAACGCGGGCCTGACGCTGGCCGGCTCGGCCGTCGCGTCGGATGCGTTCTTCCCGTTCCGCGACGGCCTCGACGTCGTC
GTGGCGGCCGGCGCGACCTGCGTGATCCAGCCGGGCGGCTCGATGCGCGACGACGAAGTGATCGCGGCGGCCGACGAGCA
CGGTATCGCGATGGTCCTGACGGGCGTGCGCCACTTCCGTCACTGA

Upstream 100 bases:

>100_bases
TCGGCATCAATCGCAATACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAGGCGGCCGTCCCGTCCCCGTTTCCC
TGGCTATTGGTGGTTCCATC

Downstream 100 bases:

>100_bases
TCGCCGCATGCGGCCCGCGCGGCCGCTGCACCGGAACCCGGCGGCATCATCCCCGCCGGGTTTTTTATTGCGCGGCGCCT
TCGCGCGCGAACGGGCGACC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTVGYPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH

Sequences:

>Translated_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTVGYPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH
>Mature_521_residues
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAIENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKAN
GNTVGYPTNFRLATKVFAHTAQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAKAFQTDPTSAFGGIIAFNREV
DEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEIALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQ
PTAKEMDDLLFAWRVAKYVKSNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=37.4476987447699, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=62.0952380952381, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=612, Percent_Identity=32.843137254902, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI71985574, Length=318, Percent_Identity=25.4716981132075, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.0769230769231, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=37.0901639344262, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=36.6666666666667, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24649832, Length=485, Percent_Identity=38.3505154639175, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55548; Mature: 55548

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTVGYPTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC
>Mature Secondary Structure
MIKQALISVSDKTGIVDFAKSLSDLGVKLLSTGGTAKLLADAGLPVTEVADYTGFPEMLD
CCCHHHEECCCCCCHHHHHHHHHHCCEEEECCCCCEEEHCCCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMQALEQHGIPTIDLLVVNLYPFVATIAKDDCTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVVVDPADYAVVLDEMKANGNTVGYPTNFRLATKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHH
AQYDGAITNYLTSLTDELKHASRSTYPATLNLAFDKVQDLRYGENPHQSAAFYRDLATPA
HHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCH
GALANYRQLQGKELSYNNIADSDAAWECVKTFDAPACVIIKHANPCGVAVGNDSADAYAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCHHHHHH
AFQTDPTSAFGGIIAFNREVDEAAAQAVAKQFVEVLIAPSFSDAAKQVFAAKQNVRLLEI
HHCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEE
ALGDGHNAFDLKRVGGGLLVQSLDSRNVQPSELRVVTKRQPTAKEMDDLLFAWRVAKYVK
EECCCCCCEEHHHCCCCCEEEECCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHH
SNAIVFCGNGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGLDVV
CCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCCEEE
VAAGATCVIQPGGSMRDDEVIAAADEHGIAMVLTGVRHFRH
EECCCEEEECCCCCCCCCCEEEECCCCCEEHEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA