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The map label for this gene is dctA2 [H]

Identifier: 116688669

GI number: 116688669

Start: 719544

End: 720863

Strand: Direct

Name: dctA2 [H]

Synonym: Bcen2424_0646

Alternate gene names: 116688669

Gene position: 719544-720863 (Clockwise)

Preceding gene: 116688667

Following gene: 116688670

Centisome position: 20.65

GC content: 65.68

Gene sequence:

>1320_bases
GTGTCGAAATTCCTCAATTCGCTGTTTGGCCGGGTAGTCGTCGCATTGATACTGGGGGTCGCGCTCGGCGCGTTCTTCCC
GCACTTCGCCGAGTCGCTACGACCGCTCGGCGACGGTTTCCTGAAGCTGATCAAGATGGTGATCGGCCCGATCGTGTTCT
GCGTCGTGGTCAGCGGGATGGCCAATGCGGGCGATCTGAAGAAGGTCGGCCGGGTCGGCCTGAAGGCCGTCGTCTACTTC
GAGGTGATGACGACGCTCGCGCTCGGCATCGGGCTCGTGCTCGCGTGGGTCACGCGCCCGGGCGTCGGGATGAACATCGA
CCTGCGCTCGCTCGACGCGGCGTCGCTGTCGACGTACGCGAAGAACGCCGAAAGCCTGAAGGACACGGCCGGCTACCTGA
TGAAGATCATCCCCGAGACCGCGATCGACGCGTTCGCGAAGGGCGACATCCTGCAGATCCTCGTGTTCGCGGTGCTGTTC
GGCTCCGCGCTGTCGCTGCTCGGCGACAAGGCGCAGCGCGTGAACAGCCTGATCGAGGAGCTGTCGCACGTGTTCTTCAA
GATCATCGGCTTCATCATCAAGCTCGCGCCGCTCGGCGTGCTCGGCGCGATCGCGTTCACGACCGGCAAGTACGGCGTCG
CGTCGCTCAAGCAGCTCGGCTACCTGGTCGCGGTGTTCTACCTGAGCTGCTTCGTGTTCGTCACGGTCGTGCTTGGCGCG
GTGATGCGGCTCGCGGGCTTCTCGGTGTTCAAGCTGATCCGCTACCTGCGCGAGGAACTGTCGATCGTGCTCGGCACGGC
GTCGTCGGACGCGGTGCTGCCGCAGGTGATGAGCAAGCTCGAATACATGGGGATCAAGGATTCGACGGTCGGCCTCGTGA
TCCCGACCGGCTATTCGTTCAACCTCGACGGCTTCTCGATCTACCTGACGCTCGCCGTGCTGTTCATCGCGCAGGCCACC
AACACGCCGCTGTCCACGCACGACCTGATCGTCGTGCTGCTCGTGTCGCTCGTCACGTCGAAGGGCGCGCACGGCATCCC
CGGCTCGGCGATCGTGATTCTCGCGGCGACGCTGTCGGCGATCCCCGCGATTCCGGTGCTCGGCCTCGTGCTGATCCTGC
CGGTCGACTGGTTCGTCGGCATCGCCCGCGCGCTGACCAACCTGATCGGCAACTGCGTCGCGACGGTGGTGGTCGCGGTG
TGGGAGAACGACATCGACCGTGCCCGCGCGAAGCGCGTGCTGAACCGCGAACTGCGCTACGTGGCAGACGCGCCGGGCGG
CACCGCCCCGGTCGCGGGCGATCAGGCCCACGCACTCTGA

Upstream 100 bases:

>100_bases
CCTTGACGCCACTATATCGGCTTCCTACTATCCACCATAACATCACTGGTCTTACCAGTATGAACAGACGAAACTGCAAC
CGTTGGAACGGGAGAGCGCC

Downstream 100 bases:

>100_bases
ACCCGACACGCGCGCGGCGCGGCTTCCCCGGCCGCCTCGCGCGACCGAATTTCCTGCGATCGGCGCGATGACGCGCCGGC
GCTTCTCAAAATAATGGAGA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSKFLNSLFGRVVVALILGVALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYF
EVMTTLALGIGLVLAWVTRPGVGMNIDLRSLDAASLSTYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLF
GSALSLLGDKAQRVNSLIEELSHVFFKIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGA
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMSKLEYMGIKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDRARAKRVLNRELRYVADAPGGTAPVAGDQAHAL

Sequences:

>Translated_439_residues
MSKFLNSLFGRVVVALILGVALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYF
EVMTTLALGIGLVLAWVTRPGVGMNIDLRSLDAASLSTYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLF
GSALSLLGDKAQRVNSLIEELSHVFFKIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGA
VMRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMSKLEYMGIKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAV
WENDIDRARAKRVLNRELRYVADAPGGTAPVAGDQAHAL
>Mature_438_residues
SKFLNSLFGRVVVALILGVALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVVYFE
VMTTLALGIGLVLAWVTRPGVGMNIDLRSLDAASLSTYAKNAESLKDTAGYLMKIIPETAIDAFAKGDILQILVFAVLFG
SALSLLGDKAQRVNSLIEELSHVFFKIIGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGYLVAVFYLSCFVFVTVVLGAV
MRLAGFSVFKLIRYLREELSIVLGTASSDAVLPQVMSKLEYMGIKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATN
TPLSTHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPAIPVLGLVLILPVDWFVGIARALTNLIGNCVATVVVAVW
ENDIDRARAKRVLNRELRYVADAPGGTAPVAGDQAHAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=456, Percent_Identity=25, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI21314632, Length=401, Percent_Identity=26.6832917705736, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI40254478, Length=484, Percent_Identity=25.4132231404959, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI4827012, Length=274, Percent_Identity=28.8321167883212, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI5032093, Length=425, Percent_Identity=25.4117647058824, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI223468564, Length=300, Percent_Identity=26.3333333333333, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI223468566, Length=300, Percent_Identity=26.3333333333333, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI66773030, Length=393, Percent_Identity=25.9541984732824, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI194239697, Length=276, Percent_Identity=28.6231884057971, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI262359914, Length=390, Percent_Identity=24.1025641025641, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI301601644, Length=165, Percent_Identity=30.3030303030303, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=51.9230769230769, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=38.8489208633094, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789473, Length=333, Percent_Identity=25.2252252252252, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=22.9426433915212, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=26.7175572519084, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=451, Percent_Identity=25.2771618625277, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=454, Percent_Identity=25.1101321585903, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=466, Percent_Identity=25.7510729613734, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=24.6305418719212, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983099, Length=450, Percent_Identity=24.2222222222222, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=450, Percent_Identity=24.2222222222222, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583025, Length=424, Percent_Identity=25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137668, Length=424, Percent_Identity=25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583023, Length=424, Percent_Identity=25, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360481, Length=481, Percent_Identity=26.6112266112266, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281360483, Length=481, Percent_Identity=26.6112266112266, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137666, Length=481, Percent_Identity=26.6112266112266, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46484; Mature: 46353

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKFLNSLFGRVVVALILGVALGAFFPHFAESLRPLGDGFLKLIKMVIGPIVFCVVVSGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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HHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]