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The map label for this gene is murD

Identifier: 116688581

GI number: 116688581

Start: 626571

End: 628082

Strand: Direct

Name: murD

Synonym: Bcen2424_0557

Alternate gene names: 116688581

Gene position: 626571-628082 (Clockwise)

Preceding gene: 116688580

Following gene: 116688582

Centisome position: 17.98

GC content: 69.97

Gene sequence:

>1512_bases
ATGTTTGGAGATCGGCAACGGCCGATGGTGCTCGTACTGGGGCTCGGGGAATCGGGTCTCGCGATCGCGCGATGGTGCGC
GAGGCACGGGTGCCGGCTGCGTATTGCCGATACCCGCGAGGCGCCGCCGAACCTTGCCGCGCTGCAGGCCGAAGGCATCG
ATGCGGAATTCGTCGGCGGGGCGTTCACGCCTGCGCTGCTCGATGGTGGCGTCGAGATCGTCGGACTGAGCCCCGGCCTG
TCGCCGCTGGAGCCGGCGCTCGCGGCGCTGGTCGCGGCCGCGAACGAGCGCGGTGTCGCGGTATGGGGCGAGCTGGAATT
CTTCGCGCAGGCGCTGCGTGCGCTCGGCACGAGCGGCTACCAGCCGAAGGTGCTCGCGATTACCGGCACCAACGGCAAGA
CCACGACGACGAGCCTCACGGGCCTGCTGTGTCAGCGTTCGGGCAAGAAGGTCGCCGTCGCGGGCAACATCAGCCCTGCG
ATGCTCGACCGGCTCGCGAGCGCGATCGACGAAACCGCGCTGCCCGACGTATGGGTGCTCGAACTGTCGAGCTTCCAGCT
CGAGACCGCGCGCACGTTTGCGCCGGACGCGGCCGCGATCCTCAACATCACGCAGGACCACCTCGACTGGCACGGCAGCT
TCGACGCGTACGCGCAGGCAAAGGGCCGGATCTTCGGCGCGACGACGACGCGCGTGCTGAACCGCGATGACGCGGCCGTG
ATGAAGTTCGCTCCCGCGGTTGCCGCGGCCGACGCGCCGCGCACGGTCACGTTCGGCCTGAACGAACCGACGCAGGACGG
CGACTACGGGCTGTCGCGCGACAACGGCATCGCATGGCTGGTGGAAGCCGTCGATCGCGACGCGCCGGATGAAGCGACCA
CGACCCGCCGGCGCAAGCGCGACGCCGCGCATACGCCCGACATCGCGCAAAAGCGCCTGATGCCGGCCGACGCGTTGCGC
ATCCGCGGGCTGCACAACGCGGCGAACGCGCTGGCCGCGTTCGCGCTCGCGCGCGCGATCGACCTTCCCGCCGCACCGTT
GCTGCACGCGCTGCGCGAATACCGCGGCGAAGCGCATCGCGTCGAAGTGATCGCGACGATCGACGACGTCGACTATGTGG
ATGACAGCAAGGGCACGAACGTCGGCGCGACGGTAGCCGCGCTCGACGGGCTCGCGCAGAAGATCGTGCTGATCGCGGGC
GGCGACGGCAAGGGGCAGGATTTCGCGCCGCTGGTCGCGCCGGTCGCGCGCTGGTGCCGCGCGGTGATGCTGATCGGCCG
CGACGCGCCGGTGATCCGCGACACGCTCGCCGAAACGGGCGTGCCGCTCGCCGACCATGCGACGCTCGAAGCAGCGGTGC
ACGCAGCAGCCGAACTCGCCGAGCCGGGCGATGCGGTGTTGCTGTCGCCTGCCTGTGCGAGCCTCGACATGTTCAGGAAC
TACGCCCATCGCGCGGAAGTGTTCCGCGCGGCGGTCGACGCAATCGCCATCGACAAAGGAGCGACGCCATGA

Upstream 100 bases:

>100_bases
GTGGTCGTGCGTTTCTGGATCATCACGCTGATGCTGTGCCTGTTCGGTCTGACCACCCTCAAGCTGCGGTAAAGGAAAGG
TAACAAGGATGTCTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTCTCCCGCTTCAATGACCGGCGCGACACCGGCGGCAATGCCGCGGGCGGCCGCGTCGCGTCG
GCCACGCGCGCCGCGACGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGVEIVGLSPGL
SPLEPALAALVAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLASAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAVAAADAPRTVTFGLNEPTQDGDYGLSRDNGIAWLVEAVDRDAPDEATTTRRRKRDAAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPVIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRN
YAHRAEVFRAAVDAIAIDKGATP

Sequences:

>Translated_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGVEIVGLSPGL
SPLEPALAALVAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLASAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAVAAADAPRTVTFGLNEPTQDGDYGLSRDNGIAWLVEAVDRDAPDEATTTRRRKRDAAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPVIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRN
YAHRAEVFRAAVDAIAIDKGATP
>Mature_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGVEIVGLSPGL
SPLEPALAALVAAANERGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLASAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAVAAADAPRTVTFGLNEPTQDGDYGLSRDNGIAWLVEAVDRDAPDEATTTRRRKRDAAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPVIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRN
YAHRAEVFRAAVDAIAIDKGATP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=496, Percent_Identity=35.4838709677419, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BURCA (Q1BZG5)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_619963.1
- ProteinModelPortal:   Q1BZG5
- SMR:   Q1BZG5
- STRING:   Q1BZG5
- GeneID:   4091836
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_0075
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02006
- BioCyc:   BCEN331271:BCEN_0075-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52852; Mature: 52852

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
AFTPALLDGGVEIVGLSPGLSPLEPALAALVAAANERGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPAMLDRLASAIDETALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECHHHHHHCCCCHHH
MKFAPAVAAADAPRTVTFGLNEPTQDGDYGLSRDNGIAWLVEAVDRDAPDEATTTRRRKR
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHH
DAAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPVIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRN
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
YAHRAEVFRAAVDAIAIDKGATP
HHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
AFTPALLDGGVEIVGLSPGLSPLEPALAALVAAANERGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPAMLDRLASAIDETALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECHHHHHHCCCCHHH
MKFAPAVAAADAPRTVTFGLNEPTQDGDYGLSRDNGIAWLVEAVDRDAPDEATTTRRRKR
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHH
DAAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPVIRDTLAETGVPLADHATLEAAVHAAAELAEPGDAVLLSPACASLDMFRN
HHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
YAHRAEVFRAAVDAIAIDKGATP
HHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA