Click here to switch to the map view.

The map label for this gene is thiE [H]

Identifier: 116688437

GI number: 116688437

Start: 465218

End: 466345

Strand: Direct

Name: thiE [H]

Synonym: Bcen2424_0413

Alternate gene names: 116688437

Gene position: 465218-466345 (Clockwise)

Preceding gene: 116688436

Following gene: 116688438

Centisome position: 13.35

GC content: 74.02

Gene sequence:

>1128_bases
ATGAGCGCGGCGCGCTTCGCCGATGCGTTCTGGCCGCCGGCCGACGAGCTGGCCGAAGCGGCCGAGCGGATTCGCGCGCG
GCTCGGCGACTGGCCCGAGGGCGCGGCGCCGTGGCGGCTCTGCGTGGCGGCGCCCGACGTGCCGGCCGATGGCGACGTGC
TGATCGTGTCGGCGGGCGACCGTGCCGCGCAGGCACGCGCATCGGCCGTGTCGCGGCCTGCGTCGCCCGATGCGGTGGCG
ATCGAATTCGACGAGCAGGGCGCCGTGCTGCATGCGGCGGGCGGGCGCTACGCGCTCGACGCCGCGCATCCGCTCGCGGA
TGACTGGATCGCGGCGCTCGCCGCGTTCCTCGATTGCGGCTTCGCGCCGGTCGACGCGCTGGTGCTCGCGCTGGCGTGGC
GCGACGGCGACGAGACGCGCGCCGCCGACGCATGGCCGGTCGATGCCGCGCGATTCCCGCGGGTGGCCGGGCTGCCGCCC
GCGCCGGAACCGGCGTTCGCGCCGTGCCCCGCGCGGCTCGGCCTCTATCCGGTCGTGCCGAGCGCCGAATGGGTCGAGCG
CGTGCTCGACGGCGGCGCGCGAACCGTGCAATTGCGCGTGAAAGAGGCGACGCCGGACGCGCTGCGCCGGGAAATCGCGC
GTGCGGTCGCGGCCGGACGCCGCTATCCCGATGCGCGCGTGTTCATCAACGATCACTGGGAGATCGCCGCAGAAGCGGGC
GCGTACGGGGTACATCTGGGCCAAGAGGACCTCGAAACGGCCGATCTGGCCGCGATCGCGGGCGCGGGCCTGCGGCTCGG
ACTTTCGAGTCACGGCTATTACGAGATGTTGCGGGCGCTGCACGAGCGGCCGAGCTACCTCGCGCTCGGCCCCGTGTACG
CGACCGCCACCAAGGCCGTTGCCGCGCCGCCACAGGGGCTCGCGCGGATTGCCCGCTACGCGCGCTTCGCCGGCGCGCGC
GCGCCGCTCGTCGCGATCGGCGGGGTGGGGCTCGACACGCTGCCGGCGGTGCTGGCGACGGGCGTGGGCAGCGTCGCGGT
GGTCAGCGCGGTCACGGGCGCGGCCGACTATCGGACGGTGCTTATTGCACTGCAGCAGTGCTTTACCGGACAATTTGACA
ATCATTGA

Upstream 100 bases:

>100_bases
GCGCCGCGTATCTCGCCGGTCCGATGGACGCGCGCGACACCGCGCATGCGAGCACGCCGGTCGTCGGGATGCCGTTCTGG
CACCAGGACGGAGGCGGAGC

Downstream 100 bases:

>100_bases
CCGCAGGGCCCGGAACGGCGTCACGACATCATTCCAATGCAGGCCCTATAATTCGGCGTTCTGCGTAAAAGGACTGTCAG
CTCCGTGAGCCCCACTCCTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSAARFADAFWPPADELAEAAERIRARLGDWPEGAAPWRLCVAAPDVPADGDVLIVSAGDRAAQARASAVSRPASPDAVA
IEFDEQGAVLHAAGGRYALDAAHPLADDWIAALAAFLDCGFAPVDALVLALAWRDGDETRAADAWPVDAARFPRVAGLPP
APEPAFAPCPARLGLYPVVPSAEWVERVLDGGARTVQLRVKEATPDALRREIARAVAAGRRYPDARVFINDHWEIAAEAG
AYGVHLGQEDLETADLAAIAGAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGAR
APLVAIGGVGLDTLPAVLATGVGSVAVVSAVTGAADYRTVLIALQQCFTGQFDNH

Sequences:

>Translated_375_residues
MSAARFADAFWPPADELAEAAERIRARLGDWPEGAAPWRLCVAAPDVPADGDVLIVSAGDRAAQARASAVSRPASPDAVA
IEFDEQGAVLHAAGGRYALDAAHPLADDWIAALAAFLDCGFAPVDALVLALAWRDGDETRAADAWPVDAARFPRVAGLPP
APEPAFAPCPARLGLYPVVPSAEWVERVLDGGARTVQLRVKEATPDALRREIARAVAAGRRYPDARVFINDHWEIAAEAG
AYGVHLGQEDLETADLAAIAGAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGAR
APLVAIGGVGLDTLPAVLATGVGSVAVVSAVTGAADYRTVLIALQQCFTGQFDNH
>Mature_374_residues
SAARFADAFWPPADELAEAAERIRARLGDWPEGAAPWRLCVAAPDVPADGDVLIVSAGDRAAQARASAVSRPASPDAVAI
EFDEQGAVLHAAGGRYALDAAHPLADDWIAALAAFLDCGFAPVDALVLALAWRDGDETRAADAWPVDAARFPRVAGLPPA
PEPAFAPCPARLGLYPVVPSAEWVERVLDGGARTVQLRVKEATPDALRREIARAVAAGRRYPDARVFINDHWEIAAEAGA
YGVHLGQEDLETADLAAIAGAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGARA
PLVAIGGVGLDTLPAVLATGVGSVAVVSAVTGAADYRTVLIALQQCFTGQFDNH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=196, Percent_Identity=55.1020408163265, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39197; Mature: 39065

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARFADAFWPPADELAEAAERIRARLGDWPEGAAPWRLCVAAPDVPADGDVLIVSAGD
CCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCC
RAAQARASAVSRPASPDAVAIEFDEQGAVLHAAGGRYALDAAHPLADDWIAALAAFLDCG
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCC
FAPVDALVLALAWRDGDETRAADAWPVDAARFPRVAGLPPAPEPAFAPCPARLGLYPVVP
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCEECCC
SAEWVERVLDGGARTVQLRVKEATPDALRREIARAVAAGRRYPDARVFINDHWEIAAEAG
CHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCC
AYGVHLGQEDLETADLAAIAGAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAV
CCEEECCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHH
AAPPQGLARIARYARFAGARAPLVAIGGVGLDTLPAVLATGVGSVAVVSAVTGAADYRTV
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
LIALQQCFTGQFDNH
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAARFADAFWPPADELAEAAERIRARLGDWPEGAAPWRLCVAAPDVPADGDVLIVSAGD
CCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCC
RAAQARASAVSRPASPDAVAIEFDEQGAVLHAAGGRYALDAAHPLADDWIAALAAFLDCG
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHCC
FAPVDALVLALAWRDGDETRAADAWPVDAARFPRVAGLPPAPEPAFAPCPARLGLYPVVP
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCEECCC
SAEWVERVLDGGARTVQLRVKEATPDALRREIARAVAAGRRYPDARVFINDHWEIAAEAG
CHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCC
AYGVHLGQEDLETADLAAIAGAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAV
CCEEECCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHH
AAPPQGLARIARYARFAGARAPLVAIGGVGLDTLPAVLATGVGSVAVVSAVTGAADYRTV
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
LIALQQCFTGQFDNH
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA