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The map label for this gene is fliA [H]

Identifier: 116688295

GI number: 116688295

Start: 312962

End: 313693

Strand: Direct

Name: fliA [H]

Synonym: Bcen2424_0271

Alternate gene names: 116688295

Gene position: 312962-313693 (Clockwise)

Preceding gene: 116688294

Following gene: 116688296

Centisome position: 8.98

GC content: 68.03

Gene sequence:

>732_bases
ATGTACAACGCTCAAGGAAAGATGTCCCAGGCCGACGTGCTCGCGCAATACGCGCCGCTCGTGCGCCGCCTCGGGCTGCA
GCTCGTCGCGAAGATGCCGGCGAGCGTCGATCTCGACGACCTGATCCAGGCCGGCATGATCGGCCTGATGGACGCGGCCG
GCCGCTACAAGGAAGACCAGGGCGCGCAGTTCGAGACCTACGCGACGCAGCGCATCCGCGGCGCGATGCTCGACGAGCTG
CGCAGCAACGACTGGCTGCCGCGCAGCCTGCGCAAGACGTCGCGCGAGGTCGAGCACGCGGTGCACCAGGTCGAGCAGCA
CCTCGGCCGCTCGGCGAGCGAGACCGAGATCGCGCAGCACCTGAACATGCCGCTCGACGAATACCAGGGGATGCTGCAGG
ACCTGCACGGCAGCCAGCTCATCTATTACGAGGATTTCGACCGCGCGGCCGATGACGAGCCGTTCCTCGACCGCTATCGC
GTCGATCACGCCGATCCGTTGTCGGCGCTGCTCGACGATCACCTGCGCGAGGCGCTCGTCGAGGCGATCGAGCGGCTGCC
GGAGCGCGAGAAGCTGCTGATGTCGCTGTACTACGAACGGGGTCTGAATCTGCGCGAGATCGGCGCGGTGCTCGAAGTGA
GCGAATCGCGCGTGTGCCAGCTGCACAGCCAGGCCGTCGCACGCCTGCGCGCGCGGCTGCGCGAGCAGGCGTGGGTCGGC
GCGGAGTCCTGA

Upstream 100 bases:

>100_bases
CGTTCGGGTGCGGGCCGCGCAGTCCACGCCGGCGGCAAGCCGTCGTATGAGGCGGGCGCGGCACACGCCGCGTGAGCGCC
ACCCGAAGGAGAGAGCCACG

Downstream 100 bases:

>100_bases
CCCGAAGAAGTCCCGACCCTTTGCCGACGGCCGCGCGCGCCGGCACCGCGCGCGTCGCGTGCCTACGCGTCGTCCTTGCG
CATTTCGCGCGCCGATTTGC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MYNAQGKMSQADVLAQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLMDAAGRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQHLGRSASETEIAQHLNMPLDEYQGMLQDLHGSQLIYYEDFDRAADDEPFLDRYR
VDHADPLSALLDDHLREALVEAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRARLREQAWVG
AES

Sequences:

>Translated_243_residues
MYNAQGKMSQADVLAQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLMDAAGRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQHLGRSASETEIAQHLNMPLDEYQGMLQDLHGSQLIYYEDFDRAADDEPFLDRYR
VDHADPLSALLDDHLREALVEAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRARLREQAWVG
AES
>Mature_243_residues
MYNAQGKMSQADVLAQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLMDAAGRYKEDQGAQFETYATQRIRGAMLDEL
RSNDWLPRSLRKTSREVEHAVHQVEQHLGRSASETEIAQHLNMPLDEYQGMLQDLHGSQLIYYEDFDRAADDEPFLDRYR
VDHADPLSALLDDHLREALVEAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRARLREQAWVG
AES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=233, Percent_Identity=53.6480686695279, Blast_Score=243, Evalue=7e-66,
Organism=Escherichia coli, GI1789448, Length=204, Percent_Identity=27.9411764705882, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27732; Mature: 27732

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNAQGKMSQADVLAQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLMDAAGRYKEDQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQHLGRSASETEIAQH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LNMPLDEYQGMLQDLHGSQLIYYEDFDRAADDEPFLDRYRVDHADPLSALLDDHLREALV
HCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRARLREQAWVG
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AES
CCC
>Mature Secondary Structure
MYNAQGKMSQADVLAQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLMDAAGRYKEDQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQHLGRSASETEIAQH
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LNMPLDEYQGMLQDLHGSQLIYYEDFDRAADDEPFLDRYRVDHADPLSALLDDHLREALV
HCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRARLREQAWVG
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AES
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]