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The map label for this gene is ilvB [H]

Identifier: 116688162

GI number: 116688162

Start: 150923

End: 152626

Strand: Reverse

Name: ilvB [H]

Synonym: Bcen2424_0138

Alternate gene names: 116688162

Gene position: 152626-150923 (Counterclockwise)

Preceding gene: 116688166

Following gene: 116688161

Centisome position: 4.38

GC content: 70.6

Gene sequence:

>1704_bases
ATGTCGCATTCCAAGGATCTCGAGCCGCACGCCACCACCGGTGCGCGACTGCTCGTCGATGCGTTGCTCGCCAATCACGT
CGAACGCGTGTTCTGCGTGCCGGGCGAGAGCTTCCTCGCCGTGCTCGATGCGCTGGCGGACGACACCGCCCGCATCCAGA
CGGTCGTCTGCCGCCACGAGGCGGCCGCCGCGAACATGGCCGAAGCGGTCGGCAAGCTGACCGGCCGCCCCGGCATCGCG
TTCGTCACGCGCGGACCCGGCGCGACCCATGCGTCGATCGGCGTGCACACCGCGTTCCAGGATTCGACGCCGATGATCCT
GTTCGTCGGCCAATGCGCACGCGAACACCTCGACCGCGAAGCCTTCCAGGAAATCGACTACCGCCGGATGTTCGGCCAGA
TGGCCAAATGGGTCGCGCAGATCGACGACCCGCGCCGCATCCCCGAATACCTGAGCCATGCGTTTCACGTCGCGACGTCC
GGCCGGCCTGGCCCGGTCGTGCTCGCGCTGCCGGAGGACGTGCTGTCCGACGCGTGCGCGCCGCAGCCGGTCGTGCCGGC
CGCGAAACGCATCGCGGCCGCGCCGTCGCCCGCGCAGATCGACGAGCTGCGCGAGCGGCTCGCGCGCGCGGAACGGCCGT
TCGCAATCGTCGGCGGCAGCGGCTGGACGCCGGACGCCTGCGCGAACCTGCGCACCTTCGTCGAACGCTGGCAATTGCCG
GTCGCGTGCGCGTTCCGCTTCCAGGACACGATCGACAACGCGCACCCGAACTACGCGGGCGACGTCGGGCTCGGGATCAA
CCCCGCGCTCGCGAAACGCATCCGCGACGCCGACCTGCTGCTCGTGATCGGGCCCCGCCTCGGCGAAGCGACGACCGGCG
GCTACACGCTGCTCGATATCCCGAAAACGCGCCAGACGCTGATCCATGTACATCAGGGCGCTGACGAACTCGGCCGCGTG
TACGCGGCCGACCTGCCGATCGTGTCGGGCATGCCCGAGATCGCCGCGCCGCTGGCCGCGCTCGAACCGCCCGAGCGGCC
TGCGTGGGCCGGCGCGGCCGCCGACTCGCACCGCGCGTACCGCGAATGGCATGCGCCGCTGCCGATGCCCGGCGACGTGC
AGCTCGGCGACGTGATGGTGCAGTTGCGCGAGCGCCTGCCGCACGACGCGATCCTGACCAACGGCGCGGGCAACTACGCG
ATCTGGCTGCATCGCCACTTCGCGTACCGGCACTTCCGTTCGCAGCTTGCGCCGACGAGCGGCGCGATGGGCTACGGGCT
GCCGGCCGCGCTCGCCGCGAAGTCGCTGTATCCGCAGCGCGCGGTTGTCGCGCTCGCGGGCGACGGCTGCTTCATGATGG
CCGGCAACGAGCTTGCGACCGCGATGCAATACGGGCTGAACATCGTCGCGATCGTCGTCAACAACGGCCATTTCGGCACG
ATCCGCATGCATCAGGAGCGCAACTACCCGGGCCGCGTGCACGGCACGGGGCTCACGAATCCCGATTTCGCCGCGTATGC
ACGTGCGTTCGGCGCGCATGGCGAGACGGTCGAGCGCACTGCCGATTTCGCGCCGGCGCTCGAACGCGCGCTGACCTGCG
GATTGCCCGCGGTGATCGAGATCCGCATCCCGCAGGACGCCAGCACGCCGGCCGCGACGCTCGAACAGATCCGCGAGCAG
GGCCGCCGCGCGCGCGGCGCATGA

Upstream 100 bases:

>100_bases
GCACGCACGCGCTCGCCGCCCCCTCAGCGTTCCGACACTTTTCAGCGCCCGGCCAACCGGCGTAAGCTGTACGTCCCGCT
ACCCAGGGAGACGGCAGCCC

Downstream 100 bases:

>100_bases
CGGTGGCCAGGCATATCGCGCCGCCCGTCGCCGCGCTGTCGCACGACGACGCGCTGGCGTACCCCGGCACGCTGCTCGCG
CCCGACGGCACGCTCGTCGC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSHSKDLEPHATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIA
FVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATS
GRPGPVVLALPEDVLSDACAPQPVVPAAKRIAAAPSPAQIDELRERLARAERPFAIVGGSGWTPDACANLRTFVERWQLP
VACAFRFQDTIDNAHPNYAGDVGLGINPALAKRIRDADLLLVIGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRV
YAADLPIVSGMPEIAAPLAALEPPERPAWAGAAADSHRAYREWHAPLPMPGDVQLGDVMVQLRERLPHDAILTNGAGNYA
IWLHRHFAYRHFRSQLAPTSGAMGYGLPAALAAKSLYPQRAVVALAGDGCFMMAGNELATAMQYGLNIVAIVVNNGHFGT
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVERTADFAPALERALTCGLPAVIEIRIPQDASTPAATLEQIREQ
GRRARGA

Sequences:

>Translated_567_residues
MSHSKDLEPHATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIA
FVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATS
GRPGPVVLALPEDVLSDACAPQPVVPAAKRIAAAPSPAQIDELRERLARAERPFAIVGGSGWTPDACANLRTFVERWQLP
VACAFRFQDTIDNAHPNYAGDVGLGINPALAKRIRDADLLLVIGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRV
YAADLPIVSGMPEIAAPLAALEPPERPAWAGAAADSHRAYREWHAPLPMPGDVQLGDVMVQLRERLPHDAILTNGAGNYA
IWLHRHFAYRHFRSQLAPTSGAMGYGLPAALAAKSLYPQRAVVALAGDGCFMMAGNELATAMQYGLNIVAIVVNNGHFGT
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVERTADFAPALERALTCGLPAVIEIRIPQDASTPAATLEQIREQ
GRRARGA
>Mature_566_residues
SHSKDLEPHATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHEAAAANMAEAVGKLTGRPGIAF
VTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDREAFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSG
RPGPVVLALPEDVLSDACAPQPVVPAAKRIAAAPSPAQIDELRERLARAERPFAIVGGSGWTPDACANLRTFVERWQLPV
ACAFRFQDTIDNAHPNYAGDVGLGINPALAKRIRDADLLLVIGPRLGEATTGGYTLLDIPKTRQTLIHVHQGADELGRVY
AADLPIVSGMPEIAAPLAALEPPERPAWAGAAADSHRAYREWHAPLPMPGDVQLGDVMVQLRERLPHDAILTNGAGNYAI
WLHRHFAYRHFRSQLAPTSGAMGYGLPAALAAKSLYPQRAVVALAGDGCFMMAGNELATAMQYGLNIVAIVVNNGHFGTI
RMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVERTADFAPALERALTCGLPAVIEIRIPQDASTPAATLEQIREQG
RRARGA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=575, Percent_Identity=24.695652173913, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI21361361, Length=548, Percent_Identity=25.9124087591241, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI87081685, Length=534, Percent_Identity=29.4007490636704, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=28.7272727272727, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=29.009009009009, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=25.4480286738351, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788716, Length=583, Percent_Identity=25.3859348198971, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=25.0437828371278, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=587, Percent_Identity=27.0868824531516, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=587, Percent_Identity=27.0868824531516, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=556, Percent_Identity=25.8992805755396, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320816, Length=545, Percent_Identity=21.651376146789, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19922626, Length=483, Percent_Identity=25.6728778467909, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61077; Mature: 60946

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSKDLEPHATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
AAAANMAEAVGKLTGRPGIAFVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDRE
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHH
AFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSGRPGPVVLALPEDVLSDACA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHHHHHCCC
PQPVVPAAKRIAAAPSPAQIDELRERLARAERPFAIVGGSGWTPDACANLRTFVERWQLP
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
VACAFRFQDTIDNAHPNYAGDVGLGINPALAKRIRDADLLLVIGPRLGEATTGGYTLLDI
CEEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEC
PKTRQTLIHVHQGADELGRVYAADLPIVSGMPEIAAPLAALEPPERPAWAGAAADSHRAY
CCHHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
REWHAPLPMPGDVQLGDVMVQLRERLPHDAILTNGAGNYAIWLHRHFAYRHFRSQLAPTS
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCC
GAMGYGLPAALAAKSLYPQRAVVALAGDGCFMMAGNELATAMQYGLNIVAIVVNNGHFGT
CCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEEECCCEEE
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVERTADFAPALERALTCGLPAVIE
EEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IRIPQDASTPAATLEQIREQGRRARGA
EECCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHSKDLEPHATTGARLLVDALLANHVERVFCVPGESFLAVLDALADDTARIQTVVCRHE
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHH
AAAANMAEAVGKLTGRPGIAFVTRGPGATHASIGVHTAFQDSTPMILFVGQCAREHLDRE
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHH
AFQEIDYRRMFGQMAKWVAQIDDPRRIPEYLSHAFHVATSGRPGPVVLALPEDVLSDACA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEECCHHHHHHCCC
PQPVVPAAKRIAAAPSPAQIDELRERLARAERPFAIVGGSGWTPDACANLRTFVERWQLP
CCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCC
VACAFRFQDTIDNAHPNYAGDVGLGINPALAKRIRDADLLLVIGPRLGEATTGGYTLLDI
CEEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEC
PKTRQTLIHVHQGADELGRVYAADLPIVSGMPEIAAPLAALEPPERPAWAGAAADSHRAY
CCHHHHHHHHHCCHHHHHHEEECCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
REWHAPLPMPGDVQLGDVMVQLRERLPHDAILTNGAGNYAIWLHRHFAYRHFRSQLAPTS
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHHHHHCCCCC
GAMGYGLPAALAAKSLYPQRAVVALAGDGCFMMAGNELATAMQYGLNIVAIVVNNGHFGT
CCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECHHHHHHHHCCCEEEEEEEECCCEEE
IRMHQERNYPGRVHGTGLTNPDFAAYARAFGAHGETVERTADFAPALERALTCGLPAVIE
EEEECCCCCCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IRIPQDASTPAATLEQIREQGRRARGA
EECCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]