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The map label for this gene is stp [H]

Identifier: 116688156

GI number: 116688156

Start: 143491

End: 145116

Strand: Direct

Name: stp [H]

Synonym: Bcen2424_0132

Alternate gene names: 116688156

Gene position: 143491-145116 (Clockwise)

Preceding gene: 116688154

Following gene: 116688158

Centisome position: 4.12

GC content: 74.23

Gene sequence:

>1626_bases
ATGGATCACCCTGTTTCAGCCGCCCCGGCCCACGTGGCCCACGCGGCGCGCCGGCGCGCATGGGTGCTGGCCGCCGTCTG
CATGGCCGCCGTCGCGCTGCCGCTGTCGTTTTCGGGCGGCGCGGTCGCGACGCCGGCGATCGGCCGCGACCTGCACGGCA
GCCCGGTCGCGATGAACTGGATCACCAACGCGTTCATGCTCGCGTTCGGCAGCTTCCTGATGGCGTCGGGTGCGCTGGCC
GACCAGTTCGGCCGCAAGCGCCTGTTCGCGATCGGCGTCGGCGGCTTCACGCTGATGTCGATCGCGCTCGCGTTCGCGCC
GTCGATGCTCGCCGTCGATCTGCTGCGCGCCGCGCAAGGGCTCGCGGCGGCCGCGGCGCTGGCCGGCGGCACGGCCGCGC
TCGCGCAGGAATTCGACGGCGCGGCGCGCACGCGTGCATTCAGCCTGCTCGGCACGACGTTCGGCATCGGGCTCGCGTTC
GGGCCCGTGATCGCCGGCGGGCTGATCGGGCATTACGGCTGGCGCGCGATCTTCGTGACGAGCGCGGTGGCCGGCGTGCT
GTCGCTCGCGTTCGGGCTGCCGCGCATGCACGAGTCGCGCGACCCGCACGCGACCGGGCTCGACTGGCCCGGCACGGCGG
CGTTCACGGCCGCGCTGTCGCTGTTCACGTTCGGCGTGATCGAGGCGCCCGCGCGCGGCTGGTCGAGCCCGCTCGTGATC
GCGCTGCTGGCCGGCGCGGCGCTCGGCGCGTGCGCATTCGTGACGATCGAGACACGCGTCGCGCGGCCGATGCTCGACCT
GTCGCTGTTCCGCATCGCGCGCTTCGTCGGCGTGCAGGTGCTGCCGGTGTCGACCTGTTGCTGCTACATCGTGCTGCTCG
TCGTGCTGCCGCTGCGCTTCATCGGCATCGACGGCTTCAGCGAGATCGACGCCGGCTGGCTGATGCTCGCGATCTCCGCG
CCGATGCTGGTCGTGCCGCTCGTCGCGGCGACGCTCACGCGCTGGCTGTCGGCCGGCGTGATTTCCGGGCTCGGGCTGCT
CGTCGCGGCGGCCGGGCTCGTGTGGCTCGGCATCGCGCTGCGCGGCGGCGCGGGGCCGGCGGCGATCGCGCCGATGCTCG
CGATCGGCGTCGGTGCCGGCATGCCGTGGGGGCTGATGGACGGGCTGTCGGTGAGCGTCGTGCCGAAGGAGCGCGCGGGG
ATGGCGACGGGCATTTTCAGTACGACGCGCGTCGCCGGCGAGGGGATTGCGCTGGCGATCGTCGGTGCGGTGCTGGCCGC
GCTCGCGCAGGCGGGGCTCGCGCACGCGGCCGGTGCCGCGGCCACGCCCGACGCAACGCTGCGTGCGGCCGCGCGGCTCG
CGACCGGCGATCTCGCGGGTGCGGCTGCCGCGTTGCCGGGCGTCGGACGTGCGGCGCTGATCGCGAGCTACACGCATGCG
TTCGGCCGGCTGCTGATCGGGCTGGCCGTCGTCACGGTGCTGTGCGCGGTGGTGGTGTTCGGGTTTCTCGGCGGGCGGCC
GGCGGCCGACGAAACGGGCGACGAGGCGGATGGCCACGCGCAGGCCGATGCGTCGGCGGCGCGCAGTCGAGGTGAGCCGG
CGTGTGTGGAGACGCAAGGGCGTTGA

Upstream 100 bases:

>100_bases
CGTTATATGCGGAGCTTCGCGTGGTTTTTCGCGGATTGAATCGGATTTATCTTCTGTCGCTCTGATGGCGCATCCGCGCC
GGCTTTTTCGAGGAGCGATG

Downstream 100 bases:

>100_bases
GCGTTGCACCGTGGCGCGCGGGTGTTTCGATGTTTCGATGTTTCGGTGCTCGGTGCTCGGTGCTCGGTGCCCGGTGCCCG
GTGCCCGGTGCCCGGTGCCC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MDHPVSAAPAHVAHAARRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGSPVAMNWITNAFMLAFGSFLMASGALA
DQFGRKRLFAIGVGGFTLMSIALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAF
GPVIAGGLIGHYGWRAIFVTSAVAGVLSLAFGLPRMHESRDPHATGLDWPGTAAFTAALSLFTFGVIEAPARGWSSPLVI
ALLAGAALGACAFVTIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISA
PMLVVPLVAATLTRWLSAGVISGLGLLVAAAGLVWLGIALRGGAGPAAIAPMLAIGVGAGMPWGLMDGLSVSVVPKERAG
MATGIFSTTRVAGEGIALAIVGAVLAALAQAGLAHAAGAAATPDATLRAAARLATGDLAGAAAALPGVGRAALIASYTHA
FGRLLIGLAVVTVLCAVVVFGFLGGRPAADETGDEADGHAQADASAARSRGEPACVETQGR

Sequences:

>Translated_541_residues
MDHPVSAAPAHVAHAARRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGSPVAMNWITNAFMLAFGSFLMASGALA
DQFGRKRLFAIGVGGFTLMSIALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAF
GPVIAGGLIGHYGWRAIFVTSAVAGVLSLAFGLPRMHESRDPHATGLDWPGTAAFTAALSLFTFGVIEAPARGWSSPLVI
ALLAGAALGACAFVTIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISA
PMLVVPLVAATLTRWLSAGVISGLGLLVAAAGLVWLGIALRGGAGPAAIAPMLAIGVGAGMPWGLMDGLSVSVVPKERAG
MATGIFSTTRVAGEGIALAIVGAVLAALAQAGLAHAAGAAATPDATLRAAARLATGDLAGAAAALPGVGRAALIASYTHA
FGRLLIGLAVVTVLCAVVVFGFLGGRPAADETGDEADGHAQADASAARSRGEPACVETQGR
>Mature_541_residues
MDHPVSAAPAHVAHAARRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGSPVAMNWITNAFMLAFGSFLMASGALA
DQFGRKRLFAIGVGGFTLMSIALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAF
GPVIAGGLIGHYGWRAIFVTSAVAGVLSLAFGLPRMHESRDPHATGLDWPGTAAFTAALSLFTFGVIEAPARGWSSPLVI
ALLAGAALGACAFVTIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISA
PMLVVPLVAATLTRWLSAGVISGLGLLVAAAGLVWLGIALRGGAGPAAIAPMLAIGVGAGMPWGLMDGLSVSVVPKERAG
MATGIFSTTRVAGEGIALAIVGAVLAALAQAGLAHAAGAAATPDATLRAAARLATGDLAGAAAALPGVGRAALIASYTHA
FGRLLIGLAVVTVLCAVVVFGFLGGRPAADETGDEADGHAQADASAARSRGEPACVETQGR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=26.5664160401003, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=23.7623762376238, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788710, Length=314, Percent_Identity=24.5222929936306, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=244, Percent_Identity=28.6885245901639, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54219; Mature: 54219

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHPVSAAPAHVAHAARRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGSPVAMNW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCEEHHH
ITNAFMLAFGSFLMASGALADQFGRKRLFAIGVGGFTLMSIALAFAPSMLAVDLLRAAQG
HHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPVIAGGLIGHYGWRAIFVT
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVAGVLSLAFGLPRMHESRDPHATGLDWPGTAAFTAALSLFTFGVIEAPARGWSSPLVI
HHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALLAGAALGACAFVTIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRF
HHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHH
IGIDGFSEIDAGWLMLAISAPMLVVPLVAATLTRWLSAGVISGLGLLVAAAGLVWLGIAL
HCCCCCCHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGAGPAAIAPMLAIGVGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAI
CCCCCHHHHHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHH
VGAVLAALAQAGLAHAAGAAATPDATLRAAARLATGDLAGAAAALPGVGRAALIASYTHA
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHH
FGRLLIGLAVVTVLCAVVVFGFLGGRPAADETGDEADGHAQADASAARSRGEPACVETQG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
R
C
>Mature Secondary Structure
MDHPVSAAPAHVAHAARRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGSPVAMNW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCEEHHH
ITNAFMLAFGSFLMASGALADQFGRKRLFAIGVGGFTLMSIALAFAPSMLAVDLLRAAQG
HHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPVIAGGLIGHYGWRAIFVT
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVAGVLSLAFGLPRMHESRDPHATGLDWPGTAAFTAALSLFTFGVIEAPARGWSSPLVI
HHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALLAGAALGACAFVTIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRF
HHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHH
IGIDGFSEIDAGWLMLAISAPMLVVPLVAATLTRWLSAGVISGLGLLVAAAGLVWLGIAL
HCCCCCCHHCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGGAGPAAIAPMLAIGVGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAI
CCCCCHHHHHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHH
VGAVLAALAQAGLAHAAGAAATPDATLRAAARLATGDLAGAAAALPGVGRAALIASYTHA
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHH
FGRLLIGLAVVTVLCAVVVFGFLGGRPAADETGDEADGHAQADASAARSRGEPACVETQG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]