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The map label for this gene is argT [H]

Identifier: 116688144

GI number: 116688144

Start: 129366

End: 130154

Strand: Direct

Name: argT [H]

Synonym: Bcen2424_0120

Alternate gene names: 116688144

Gene position: 129366-130154 (Clockwise)

Preceding gene: 116688143

Following gene: 116688145

Centisome position: 3.71

GC content: 66.79

Gene sequence:

>789_bases
ATGAAACACACGGCTACCTTGAAACTCGCGTTCGCACTCGCGTGCGCCATCGGCTCCGGCGCGGCGCTCGCCGATGCGCC
GACGCTGCGCTTCGGTCTCGAAGCGCAATACCCGCCGTTCGAATCGAAGGCGCCGAACGGCGACCTGCAGGGCTTCGACA
TCGACGTCGGCAACGCCGTCTGCCAGACGGCGAAGCTGTCGTGCAAATGGGTCGAGACGTCGTTCGACGGGCTGATTCCG
GCGCTGAAGGGCCGCAAGTTCGACGCGATCAACTCGGCGATGAACGCGACCGAGCAACGGCGCCAGGCGATCGACTTCAC
CACGATCATCTATCGCGTGCCGACCCAGCTGATCGCGCGCACCGGCAGCGGGCTGCTGCCGACGCCCGAATCGCTGAAGG
GCAAGCGCGTGGGCGTGCTGCAGGCGTCGATCCAGGAAACCTTCGCGAAGGCGCACTGGGAGCCGGCCGGCGTCGCGATC
GTCGCGTACCAGGACCAGAACCAGGCGTATGCGGATCTGGTCGCGGGCCGCCTCGACGCGACGCTCGTCCTCGCGCCGTC
GGGCCAGCGAGGCTTCCTGTCGCGCCCGGACGGCAAGGGCTTCTCGTTCGTCGGCCAGCCGGTGCACGACGACAAGATCC
TCGGCAGCGGCATCGCGTTCGGCCTGCGCAAGGGCGACGACGCGCTGAAGGCGAAGCTCGACGCGGCGATCGACAAGCTG
AAGGCGGACGGCACGGTGAAGTCGCTCGGGTTGAAGTATTTCGGCGATATCGACATCTCGACGAAGTAA

Upstream 100 bases:

>100_bases
CGCGACGCTGGTCGAAGGCGCGCGGCGGCTGCGTTCGCTGTAAGGCCCATACGGAAACCGGCGACGGAGACGTCGCATCG
ACCAGACCCAGGAGATCACG

Downstream 100 bases:

>100_bases
GCGGGGCCGCGCACGATGCCGACCTCGAACCGGACCGCTGCGGGCAGCGCACCGGACGGCAGGCCGCTGCCGGACGAGGC
CGTCGATGCCGTGCTCGCCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKHTATLKLAFALACAIGSGAALADAPTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCQTAKLSCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVHDDKILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISTK

Sequences:

>Translated_262_residues
MKHTATLKLAFALACAIGSGAALADAPTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCQTAKLSCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVHDDKILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISTK
>Mature_262_residues
MKHTATLKLAFALACAIGSGAALADAPTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCQTAKLSCKWVETSFDGLIP
ALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAI
VAYQDQNQAYADLVAGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVHDDKILGSGIAFGLRKGDDALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISTK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=45.4545454545455, Blast_Score=220, Evalue=6e-59,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=45.6896551724138, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=32.6612903225806, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=32.3770491803279, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=29.4372294372294, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787031, Length=258, Percent_Identity=29.0697674418605, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27811; Mature: 27811

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTATLKLAFALACAIGSGAALADAPTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAV
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCHHH
CQTAKLSCKWVETSFDGLIPALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAIVAYQDQNQAYADLVAGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCCCE
TLVLAPSGQRGFLSRPDGKGFSFVGQPVHDDKILGSGIAFGLRKGDDALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
KADGTVKSLGLKYFGDIDISTK
CCCCCHHHCCCEEECCEEECCC
>Mature Secondary Structure
MKHTATLKLAFALACAIGSGAALADAPTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAV
CCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCHHH
CQTAKLSCKWVETSFDGLIPALKGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHCEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAIVAYQDQNQAYADLVAGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHCCCCE
TLVLAPSGQRGFLSRPDGKGFSFVGQPVHDDKILGSGIAFGLRKGDDALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
KADGTVKSLGLKYFGDIDISTK
CCCCCHHHCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]