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The map label for this gene is purR [H]

Identifier: 116626770

GI number: 116626770

Start: 9721922

End: 9722932

Strand: Direct

Name: purR [H]

Synonym: Acid_7743

Alternate gene names: 116626770

Gene position: 9721922-9722932 (Clockwise)

Preceding gene: 116626769

Following gene: 116626771

Centisome position: 97.55

GC content: 62.41

Gene sequence:

>1011_bases
ATGCCGAGCATCAAGAAGGTGGCCGAGGTAGCCGGTGTTTCGGTGGGGACGGTCTCCCACGTGATCACCGGCTCGGTCAA
CGTGAGTGAGCCGCTGCGGATGAAAGTTCAGGCTGCGATCCGAGAGCTGAACTACCATCCGAATCACGTGGCGCGCAGCC
TGAAGACCAGCAAGACGCGCACGCTTGGAATCATCGTTCCGGACCTGACGATCTCCTTCTTCCCACAGATTATTCGCGGC
GCGGAAACCGCCGCCAGGCAGCGCCGCTATTCGATTATCGCGGTGAACTCAGACGATGATGGCGAGCGCCAGAAGGAGCT
GCTCTCGTTGTTGCACTCGCAGCGGGTGGAAGGAATCCTGCTGGTGATTGCCGCCGCGCCTACGCCGCAGAGCCAGATCG
GGCGGATTCTCGACGCCGATATTCCGGTGGTCTGCCTGGATCGCGTCCCCGACCGGATTGCGGTGGACTCGGTCTCGGTG
GACGACTTGGAGGCCGCGCGAATGGGTGTCGCGCACCTGGTGGAGCGCGGGTTCCGCCGCATTGCGATCATGACTGGCCC
GATGTCGTTAAGAAATGAGCGGCGGCGCCTTCAGGGCTACCGGCAGGCGCTGGACGAAGCCGGCATCAAGATCGACGAGC
GCCTGATCTGGAACGGCACGCTGCATCTGGAGGAAGTGACCGCGCTCTGCCGCGAACGCCTGTGCGATAAGGCGTCGCGC
CCCGACGCGTTGTTTTGCACCAACGGACACAATGCGCTCGGCGTGCTGCGCGCCTTTCGCGATTGCGGACTGGAGACACC
GCGCGATATCGGCTTTCTCACCTTCGACGAGCTCACGGTAGAAGATCTTTTCACGCCTTCGATTACGACGATCGTGCAGC
CGGCTTACGACATCGGATACCGGGCGGGCATGATCCTGCTGGATCGGATCGAAGGTCTGGCCACACAGGAGAGTTCGATC
ACGGTGCGACTGCCGGCGACGCTGAAAATTCGGGCGTCGACGAGGCGGTAA

Upstream 100 bases:

>100_bases
AAAGAGGGGCGCAAGATGGACGGCCTGATCGGGATTCAGGTGCACAAGGGACCGCCCATGAAGATCGAGGTCCGCAACAT
CCGTTTGAAGAAGCTGTAGA

Downstream 100 bases:

>100_bases
CGCCATTCCCTTGCAATATCTTGCGAAATTTTGAGCTACTTTCCCCGACTTTCTCGCGTTAATTAAGAGGAAAGAGGGAG
TTGCTTTGACAGCAGTACGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPSIKKVAEVAGVSVGTVSHVITGSVNVSEPLRMKVQAAIRELNYHPNHVARSLKTSKTRTLGIIVPDLTISFFPQIIRG
AETAARQRRYSIIAVNSDDDGERQKELLSLLHSQRVEGILLVIAAAPTPQSQIGRILDADIPVVCLDRVPDRIAVDSVSV
DDLEAARMGVAHLVERGFRRIAIMTGPMSLRNERRRLQGYRQALDEAGIKIDERLIWNGTLHLEEVTALCRERLCDKASR
PDALFCTNGHNALGVLRAFRDCGLETPRDIGFLTFDELTVEDLFTPSITTIVQPAYDIGYRAGMILLDRIEGLATQESSI
TVRLPATLKIRASTRR

Sequences:

>Translated_336_residues
MPSIKKVAEVAGVSVGTVSHVITGSVNVSEPLRMKVQAAIRELNYHPNHVARSLKTSKTRTLGIIVPDLTISFFPQIIRG
AETAARQRRYSIIAVNSDDDGERQKELLSLLHSQRVEGILLVIAAAPTPQSQIGRILDADIPVVCLDRVPDRIAVDSVSV
DDLEAARMGVAHLVERGFRRIAIMTGPMSLRNERRRLQGYRQALDEAGIKIDERLIWNGTLHLEEVTALCRERLCDKASR
PDALFCTNGHNALGVLRAFRDCGLETPRDIGFLTFDELTVEDLFTPSITTIVQPAYDIGYRAGMILLDRIEGLATQESSI
TVRLPATLKIRASTRR
>Mature_335_residues
PSIKKVAEVAGVSVGTVSHVITGSVNVSEPLRMKVQAAIRELNYHPNHVARSLKTSKTRTLGIIVPDLTISFFPQIIRGA
ETAARQRRYSIIAVNSDDDGERQKELLSLLHSQRVEGILLVIAAAPTPQSQIGRILDADIPVVCLDRVPDRIAVDSVSVD
DLEAARMGVAHLVERGFRRIAIMTGPMSLRNERRRLQGYRQALDEAGIKIDERLIWNGTLHLEEVTALCRERLCDKASRP
DALFCTNGHNALGVLRAFRDCGLETPRDIGFLTFDELTVEDLFTPSITTIVQPAYDIGYRAGMILLDRIEGLATQESSIT
VRLPATLKIRASTRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=310, Percent_Identity=34.8387096774194, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787948, Length=325, Percent_Identity=34.1538461538462, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=32.3529411764706, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=29.5918367346939, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788474, Length=295, Percent_Identity=29.8305084745763, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=30.6547619047619, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787580, Length=335, Percent_Identity=27.4626865671642, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=27.7227722772277, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=27.3311897106109, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=27.5316455696203, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=27.5641025641026, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=29.2168674698795, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789456, Length=348, Percent_Identity=24.1379310344828, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37161; Mature: 37030

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIKKVAEVAGVSVGTVSHVITGSVNVSEPLRMKVQAAIRELNYHPNHVARSLKTSKTR
CCCHHHHHHHHCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCE
TLGIIVPDLTISFFPQIIRGAETAARQRRYSIIAVNSDDDGERQKELLSLLHSQRVEGIL
EEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LVIAAAPTPQSQIGRILDADIPVVCLDRVPDRIAVDSVSVDDLEAARMGVAHLVERGFRR
EEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHE
IAIMTGPMSLRNERRRLQGYRQALDEAGIKIDERLIWNGTLHLEEVTALCRERLCDKASR
EEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCEEEECCCEEHHHHHHHHHHHHHHCCCC
PDALFCTNGHNALGVLRAFRDCGLETPRDIGFLTFDELTVEDLFTPSITTIVQPAYDIGY
CCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCHHHHHCCHHHCCH
RAGMILLDRIEGLATQESSITVRLPATLKIRASTRR
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCC
>Mature Secondary Structure 
PSIKKVAEVAGVSVGTVSHVITGSVNVSEPLRMKVQAAIRELNYHPNHVARSLKTSKTR
CCHHHHHHHHCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCE
TLGIIVPDLTISFFPQIIRGAETAARQRRYSIIAVNSDDDGERQKELLSLLHSQRVEGIL
EEEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LVIAAAPTPQSQIGRILDADIPVVCLDRVPDRIAVDSVSVDDLEAARMGVAHLVERGFRR
EEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHE
IAIMTGPMSLRNERRRLQGYRQALDEAGIKIDERLIWNGTLHLEEVTALCRERLCDKASR
EEEEECCHHHHHHHHHHHHHHHHHHHCCCEECCEEEECCCEEHHHHHHHHHHHHHHCCCC
PDALFCTNGHNALGVLRAFRDCGLETPRDIGFLTFDELTVEDLFTPSITTIVQPAYDIGY
CCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCHHHHHCCHHHCCH
RAGMILLDRIEGLATQESSITVRLPATLKIRASTRR
HHHHHHHHHHHCCCCCCCCEEEEECCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA