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The map label for this gene is thiG

Identifier: 116626758

GI number: 116626758

Start: 9706058

End: 9706837

Strand: Direct

Name: thiG

Synonym: Acid_7731

Alternate gene names: 116626758

Gene position: 9706058-9706837 (Clockwise)

Preceding gene: 116626757

Following gene: 116626762

Centisome position: 97.4

GC content: 61.79

Gene sequence:

>780_bases
ATGTCGGACAAATTGACCATCGCGGGACGCGAGTTTGCTTCGCGCCTGATTGTGGGCACGGGAAAATATCGCAGTTTTCA
GGAAATGCAGCGCTGCCACGAGGCGAGCGGCGCCGATATGGTGACGGTGGCGGTGCGGCGCGTAAACCTGACTGACCGTA
CCAAGGAATCGCTGATCGATTATATCGACCGCAGTAAGATTTTCATTCTGCCGAATACCGCGGGCTGTTATACCGCCGAT
GATGCTGTGCGCACTGCCATGCTGGGGCGCGAGGTCGGGCTCTCCAACTGGGTGAAGCTGGAAGTGATCGGTGACGAGAA
GACTCTGTTCCCGGATAATGCCGGGCTTCTGGAAGCGACGCGGATTCTAGTGAAGGAAGGCTTTGCGGTGCTGCCGTATA
CGAACGACGATATCGTAAACGCGCGGCGGCTGATCGAGGCGGGGGCGGCGGCGGTGATGCCGCTGGCAGCGCCGATCGGG
TCGGGGCTGGGAATTCAGAATCCGACGAATTTGCGGATCTTCCGGGAGATGATCACGGAGGTTCCGGTGATTGTGGACGC
GGGTGTGGGCACGGCGAGCGATGCGGCGATCGCGATGGAGCTGGGCGCGGACGGCGTGCTTATGAACACCGCGATTGCGG
CCGCGGAGGACGCGGTGAAGATGGCGCGCGCAATGAAGCTGGGCGTGGAGTGCGGGCGGCTGGCGTTTGAATCGGGGCGG
ATGGCGAAGAAGCTTTATGCGAGCGCGAGCAGTCCGCTGACTGGAGTGGTGGGGGCGTAG

Upstream 100 bases:

>100_bases
TTCGAAGTCGCTCGATGGGCGCAGCGTGTTTGACGATCGGAAGGGGACGAAGGGTCGGGGTTCGGCGCAGCTTTCGCTGT
TCCAGTGAGACAATGAAGGC

Downstream 100 bases:

>100_bases
GGCAGGATTAGCGAACGCTCCCTAAACGGTCGCGGCTCGGATTGAGCGTGGTGTTGATGGTTGGGGAGTGGAGTGTGGCG
AGGCAGTTCGGTCTCCCTCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSDKLTIAGREFASRLIVGTGKYRSFQEMQRCHEASGADMVTVAVRRVNLTDRTKESLIDYIDRSKIFILPNTAGCYTAD
DAVRTAMLGREVGLSNWVKLEVIGDEKTLFPDNAGLLEATRILVKEGFAVLPYTNDDIVNARRLIEAGAAAVMPLAAPIG
SGLGIQNPTNLRIFREMITEVPVIVDAGVGTASDAAIAMELGADGVLMNTAIAAAEDAVKMARAMKLGVECGRLAFESGR
MAKKLYASASSPLTGVVGA

Sequences:

>Translated_259_residues
MSDKLTIAGREFASRLIVGTGKYRSFQEMQRCHEASGADMVTVAVRRVNLTDRTKESLIDYIDRSKIFILPNTAGCYTAD
DAVRTAMLGREVGLSNWVKLEVIGDEKTLFPDNAGLLEATRILVKEGFAVLPYTNDDIVNARRLIEAGAAAVMPLAAPIG
SGLGIQNPTNLRIFREMITEVPVIVDAGVGTASDAAIAMELGADGVLMNTAIAAAEDAVKMARAMKLGVECGRLAFESGR
MAKKLYASASSPLTGVVGA
>Mature_258_residues
SDKLTIAGREFASRLIVGTGKYRSFQEMQRCHEASGADMVTVAVRRVNLTDRTKESLIDYIDRSKIFILPNTAGCYTADD
AVRTAMLGREVGLSNWVKLEVIGDEKTLFPDNAGLLEATRILVKEGFAVLPYTNDDIVNARRLIEAGAAAVMPLAAPIGS
GLGIQNPTNLRIFREMITEVPVIVDAGVGTASDAAIAMELGADGVLMNTAIAAAEDAVKMARAMKLGVECGRLAFESGRM
AKKLYASASSPLTGVVGA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=255, Percent_Identity=46.6666666666667, Blast_Score=215, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_SOLUE (Q01NZ1)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_828914.1
- ProteinModelPortal:   Q01NZ1
- SMR:   Q01NZ1
- STRING:   Q01NZ1
- GeneID:   4431347
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_7731
- NMPDR:   fig|234267.9.peg.7334
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   PIIIDAG
- PhylomeDB:   Q01NZ1
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27541; Mature: 27410

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: ACT_SITE 99-99 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLTIAGREFASRLIVGTGKYRSFQEMQRCHEASGADMVTVAVRRVNLTDRTKESLID
CCCCEEECCHHHHHHHEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
YIDRSKIFILPNTAGCYTADDAVRTAMLGREVGLSNWVKLEVIGDEKTLFPDNAGLLEAT
HHCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCCHHHHH
RILVKEGFAVLPYTNDDIVNARRLIEAGAAAVMPLAAPIGSGLGIQNPTNLRIFREMITE
HHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VPVIVDAGVGTASDAAIAMELGADGVLMNTAIAAAEDAVKMARAMKLGVECGRLAFESGR
CCEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
MAKKLYASASSPLTGVVGA
HHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDKLTIAGREFASRLIVGTGKYRSFQEMQRCHEASGADMVTVAVRRVNLTDRTKESLID
CCCEEECCHHHHHHHEECCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
YIDRSKIFILPNTAGCYTADDAVRTAMLGREVGLSNWVKLEVIGDEKTLFPDNAGLLEAT
HHCCCEEEEEECCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCCHHHHH
RILVKEGFAVLPYTNDDIVNARRLIEAGAAAVMPLAAPIGSGLGIQNPTNLRIFREMITE
HHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VPVIVDAGVGTASDAAIAMELGADGVLMNTAIAAAEDAVKMARAMKLGVECGRLAFESGR
CCEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
MAKKLYASASSPLTGVVGA
HHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA