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The map label for this gene is vanX [H]

Identifier: 116626589

GI number: 116626589

Start: 9460739

End: 9461452

Strand: Direct

Name: vanX [H]

Synonym: Acid_7552

Alternate gene names: 116626589

Gene position: 9460739-9461452 (Clockwise)

Preceding gene: 116626588

Following gene: 116626593

Centisome position: 94.93

GC content: 63.45

Gene sequence:

>714_bases
ATGCACCGGCTGCGCACCCTCGTCTTTCTCGCCGCTCTCGCCTTGACCGCGCAGGAACGCGCGCGGATTTTTCGCATCAC
GCCGGTTCGTCCCGTGGCCGAGCTGCGGGCGGAGGCGCTGGCCGCCCAGCCGCCCCAGGAAACGGGCGACTTTCTACCCT
CCTCGCTCACCGACCTCGCCACGCTCGATCACGGAATCAAGTTCGAGATTCGCTACGCGACCACCGACAACTTTCTCAGC
ACGCCGGTTTACACTTCGGCGCGTGCATTCCTGCAACGTCCCGCGGCCGAGGCTCTGTTACGCGCCCACGAAGTGTTAAG
GAAGCAGGGCTACGGGCTGCTGGTCTTCGATGCCTACCGGCCGTGGTACGTCACCAGGATATTTTGGGACGCGACGCCGC
CGGACAAACACGAATTCGTGGCCAACCCTGCGCAGGGTTCGCGCCACAACCGCGGCTGCGCCGTGGACCTTACGCTGTAC
GACCTCAAGTCCGGCAAGGAAGTCGAAATGACTGGGGTGTACGACGAAATGTCGGAGCGCTCGTACCCTAACTACACGGG
CGGCACGGCGGAGCAGCGCGCCCGGCGCGATTTATTGCGCACCGCCATGGAGAAGCAGGGCTTCAAGGTCTTCGCGTCGG
AGTGGTGGCACTTCGATTACAGCGGCTGGCAGCGGTATGCCATTCAGAATGCACCCTTCGAAGCGATTCGGTAG

Upstream 100 bases:

>100_bases
GCTGCTCTCGGGACTCGCCCCCGCGCGAAAAGCCGCGAAGACCGACCCCGCCGTAACACTGCGCGCTGAGTAGCGCCGTG
CTCGATTGTATGATGACGGG

Downstream 100 bases:

>100_bases
TATTGCGACCTGATTCACACGCTACGTCGCCAGAAGCAGTCCCCTCTCGATAGCCAATCTTCGAATGATCGATCCGAGTT
CGAGAAATTCCATCAGGTCT

Product: peptidase M15D, vanX D-ala-D-ala dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MHRLRTLVFLAALALTAQERARIFRITPVRPVAELRAEALAAQPPQETGDFLPSSLTDLATLDHGIKFEIRYATTDNFLS
TPVYTSARAFLQRPAAEALLRAHEVLRKQGYGLLVFDAYRPWYVTRIFWDATPPDKHEFVANPAQGSRHNRGCAVDLTLY
DLKSGKEVEMTGVYDEMSERSYPNYTGGTAEQRARRDLLRTAMEKQGFKVFASEWWHFDYSGWQRYAIQNAPFEAIR

Sequences:

>Translated_237_residues
MHRLRTLVFLAALALTAQERARIFRITPVRPVAELRAEALAAQPPQETGDFLPSSLTDLATLDHGIKFEIRYATTDNFLS
TPVYTSARAFLQRPAAEALLRAHEVLRKQGYGLLVFDAYRPWYVTRIFWDATPPDKHEFVANPAQGSRHNRGCAVDLTLY
DLKSGKEVEMTGVYDEMSERSYPNYTGGTAEQRARRDLLRTAMEKQGFKVFASEWWHFDYSGWQRYAIQNAPFEAIR
>Mature_237_residues
MHRLRTLVFLAALALTAQERARIFRITPVRPVAELRAEALAAQPPQETGDFLPSSLTDLATLDHGIKFEIRYATTDNFLS
TPVYTSARAFLQRPAAEALLRAHEVLRKQGYGLLVFDAYRPWYVTRIFWDATPPDKHEFVANPAQGSRHNRGCAVDLTLY
DLKSGKEVEMTGVYDEMSERSYPNYTGGTAEQRARRDLLRTAMEKQGFKVFASEWWHFDYSGWQRYAIQNAPFEAIR

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=166, Percent_Identity=34.9397590361446, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 27150; Mature: 27150

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLRTLVFLAALALTAQERARIFRITPVRPVAELRAEALAAQPPQETGDFLPSSLTDLA
CCHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
TLDHGIKFEIRYATTDNFLSTPVYTSARAFLQRPAAEALLRAHEVLRKQGYGLLVFDAYR
HHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCC
PWYVTRIFWDATPPDKHEFVANPAQGSRHNRGCAVDLTLYDLKSGKEVEMTGVYDEMSER
CEEEEEEEECCCCCCCHHEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCHHHHHHC
SYPNYTGGTAEQRARRDLLRTAMEKQGFKVFASEWWHFDYSGWQRYAIQNAPFEAIR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHEECCCCCHHHHHCCCCCHHHCC
>Mature Secondary Structure
MHRLRTLVFLAALALTAQERARIFRITPVRPVAELRAEALAAQPPQETGDFLPSSLTDLA
CCHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
TLDHGIKFEIRYATTDNFLSTPVYTSARAFLQRPAAEALLRAHEVLRKQGYGLLVFDAYR
HHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCC
PWYVTRIFWDATPPDKHEFVANPAQGSRHNRGCAVDLTLYDLKSGKEVEMTGVYDEMSER
CEEEEEEEECCCCCCCHHEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCHHHHHHC
SYPNYTGGTAEQRARRDLLRTAMEKQGFKVFASEWWHFDYSGWQRYAIQNAPFEAIR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHEECCCCCHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]