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The map label for this gene is pilR [H]

Identifier: 116626446

GI number: 116626446

Start: 9294168

End: 9295154

Strand: Direct

Name: pilR [H]

Synonym: Acid_7408

Alternate gene names: 116626446

Gene position: 9294168-9295154 (Clockwise)

Preceding gene: 116626445

Following gene: 116626447

Centisome position: 93.26

GC content: 63.63

Gene sequence:

>987_bases
ATGACTCACGGTCTTGTAGGCGGGTCCGCGCAGATTGCCTCGATTCGCCGGCTGATCGAGAAGGCGTCGCGGAACCGCCT
TCCTGTGTTGCTGTTAGGCGAAAGCGGCACGGGGAAGGAGGTGGTGGCGCGGGGCATACATAACGCCAATCCGCGGGGCG
CCTTCGTGCCGATCGATTGCGGGTCGCTGGTGGGCACGTTGATGGAGAGCGAGCTTTTCGGCCACGTGAAGGGGTCGTTC
AGCGGCGCGGCGGAGAATAAGCGCGGGTTGGTGGAACTGGCCGACGGCGGCACGGCGTTCTTCGACGAGATCGGCGACCT
GCCGCTCGAGATGCAGGTGAAGCTGCTGCGCCTGATCCAGGAGCGCGAATTTCGCGCGGTGGGGTCGCTGCAGTGGAAGA
AGGTGGATCTTCGGATTATTGCCGCGACCCATCGCGATCTGCAGGCCGAAGTGGTCAAGGGGCATTTTCGACAGGACCTG
TTCTACCGGCTCAACGTCTTCGCCATCCGGTTACCGGCCCTGCGCGATCGCAAGGAAGACATCCCGAGCCTGGTGGCGCA
TTTCGTGAATGCCGCGGAGCAGGCGGGATTGCCTGGGATCGAGCCTACCGAGGAACTGATGGCCGCCCTACACGTCTACG
ATTGGCCGGGGAATGTGCGCGAATTGAAGCATGCGATCGACCGCCTTAGCGCGCTGCGGTCCGAGGGGGCGCTGATGCAG
ATGTCGGACCTGCCGACCGCATTGCAATACCATCAGTCGGCGGCGGAAATTCGGCAGCTCTCCGACGCGCTCGAGCAACC
CGAGGCGGCGCTGCCGGAGTTTTCACTGGCGTCGCCTTCACCGGTGATCTCGCTGCCCGATAGCGAGCGGCGCGCGATCA
CTAGGGCGCTCGCGTCCACGGGAGGCGCGATCACCAAGACAGCACACCTGCTTGGGATCGGGCGCACCACTCTTTACCGG
AAAATGAGACGATACGGTATCGAGTAG

Upstream 100 bases:

>100_bases
GAGGCGGCCGGGCAACTCGCCGCCCGCATCCGGCAAGATTCTGTTGAGAAAAGTACCGGCTTCATCGTACGAATTGATTC
GATGTAAGTTGGGACCGGTT

Downstream 100 bases:

>100_bases
CGTTTGGGTGAACAGGAAGAGGGGAGCGAGGTGGCGCGCATGGCGAAGAAGCCGCCTGAAAGGCGGCTGCAGCCAGGATT
GGCTGCCCCACAGTGCGGCA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTHGLVGGSAQIASIRRLIEKASRNRLPVLLLGESGTGKEVVARGIHNANPRGAFVPIDCGSLVGTLMESELFGHVKGSF
SGAAENKRGLVELADGGTAFFDEIGDLPLEMQVKLLRLIQEREFRAVGSLQWKKVDLRIIAATHRDLQAEVVKGHFRQDL
FYRLNVFAIRLPALRDRKEDIPSLVAHFVNAAEQAGLPGIEPTEELMAALHVYDWPGNVRELKHAIDRLSALRSEGALMQ
MSDLPTALQYHQSAAEIRQLSDALEQPEAALPEFSLASPSPVISLPDSERRAITRALASTGGAITKTAHLLGIGRTTLYR
KMRRYGIE

Sequences:

>Translated_328_residues
MTHGLVGGSAQIASIRRLIEKASRNRLPVLLLGESGTGKEVVARGIHNANPRGAFVPIDCGSLVGTLMESELFGHVKGSF
SGAAENKRGLVELADGGTAFFDEIGDLPLEMQVKLLRLIQEREFRAVGSLQWKKVDLRIIAATHRDLQAEVVKGHFRQDL
FYRLNVFAIRLPALRDRKEDIPSLVAHFVNAAEQAGLPGIEPTEELMAALHVYDWPGNVRELKHAIDRLSALRSEGALMQ
MSDLPTALQYHQSAAEIRQLSDALEQPEAALPEFSLASPSPVISLPDSERRAITRALASTGGAITKTAHLLGIGRTTLYR
KMRRYGIE
>Mature_327_residues
THGLVGGSAQIASIRRLIEKASRNRLPVLLLGESGTGKEVVARGIHNANPRGAFVPIDCGSLVGTLMESELFGHVKGSFS
GAAENKRGLVELADGGTAFFDEIGDLPLEMQVKLLRLIQEREFRAVGSLQWKKVDLRIIAATHRDLQAEVVKGHFRQDLF
YRLNVFAIRLPALRDRKEDIPSLVAHFVNAAEQAGLPGIEPTEELMAALHVYDWPGNVRELKHAIDRLSALRSEGALMQM
SDLPTALQYHQSAAEIRQLSDALEQPEAALPEFSLASPSPVISLPDSERRAITRALASTGGAITKTAHLLGIGRTTLYRK
MRRYGIE

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=330, Percent_Identity=42.7272727272727, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=324, Percent_Identity=43.5185185185185, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=41.0179640718563, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI1788905, Length=318, Percent_Identity=40.5660377358491, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082117, Length=306, Percent_Identity=44.7712418300654, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1789087, Length=331, Percent_Identity=39.2749244712991, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=41.2844036697248, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=337, Percent_Identity=37.0919881305638, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=338, Percent_Identity=36.6863905325444, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=34.6385542168675, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081872, Length=206, Percent_Identity=42.7184466019417, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI87081858, Length=318, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789828, Length=237, Percent_Identity=38.8185654008439, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35919; Mature: 35788

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGLVGGSAQIASIRRLIEKASRNRLPVLLLGESGTGKEVVARGIHNANPRGAFVPIDC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCEEECCH
GSLVGTLMESELFGHVKGSFSGAAENKRGLVELADGGTAFFDEIGDLPLEMQVKLLRLIQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHH
EREFRAVGSLQWKKVDLRIIAATHRDLQAEVVKGHFRQDLFYRLNVFAIRLPALRDRKED
HHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCHHHH
IPSLVAHFVNAAEQAGLPGIEPTEELMAALHVYDWPGNVRELKHAIDRLSALRSEGALMQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
MSDLPTALQYHQSAAEIRQLSDALEQPEAALPEFSLASPSPVISLPDSERRAITRALAST
HHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHC
GGAITKTAHLLGIGRTTLYRKMRRYGIE
CCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
THGLVGGSAQIASIRRLIEKASRNRLPVLLLGESGTGKEVVARGIHNANPRGAFVPIDC
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCEEECCH
GSLVGTLMESELFGHVKGSFSGAAENKRGLVELADGGTAFFDEIGDLPLEMQVKLLRLIQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHH
EREFRAVGSLQWKKVDLRIIAATHRDLQAEVVKGHFRQDLFYRLNVFAIRLPALRDRKED
HHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCHHHH
IPSLVAHFVNAAEQAGLPGIEPTEELMAALHVYDWPGNVRELKHAIDRLSALRSEGALMQ
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEE
MSDLPTALQYHQSAAEIRQLSDALEQPEAALPEFSLASPSPVISLPDSERRAITRALAST
HHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHC
GGAITKTAHLLGIGRTTLYRKMRRYGIE
CCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]