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The map label for this gene is ydaP [H]

Identifier: 116626364

GI number: 116626364

Start: 9195663

End: 9197468

Strand: Direct

Name: ydaP [H]

Synonym: Acid_7324

Alternate gene names: 116626364

Gene position: 9195663-9197468 (Clockwise)

Preceding gene: 116626345

Following gene: 116626366

Centisome position: 92.27

GC content: 63.68

Gene sequence:

>1806_bases
GTGTGCCTTACGGTGCAGAGCCGCCTCGCGCCCGCGGTCTATGATCGGGGCATGGCACTCACCGGCGGCGACGTTCTGGT
TGAACGCATTATAGATTGGGGAGTCGAGGTGGTCTTCGGACTTCCCGGCGACGGCATCAACGGCATCATGGAAGCTCTCC
GCACCCGCCAGGAGAAGATTCGCTTCATCCAGGTGCGTCATGAGGAATCGGCCGCGTTCATGGCCTGCGCCTACGCCAAG
TACACCGGCCGGCTCGGCGTTTGCCTGGCGACCTCCGGACCCGGCGGCATTCACCTGCTCAACGGCCTCTACGACGCCAA
GATGGATAGCCAGCCCGTGTTGGCCATTACCGGCATGCACTTCCATGACCTGATCGGAACGCTGGCACAACAGGACGTCG
AACTCGACAAACTCTTCCAGGATGTCGCGGCCTTCAATCAGCGCGTAATGTCGCCCGACCATGTCGAGGGCTTGGCCGAT
CTGGCCTGCCGCACCGCGATCGACTACCGGCAGGTGGCTCACATCACCTTCCCCACCGATTTGCAGGATAAGGAGGTCTC
TAAGAAGGAGCGCTCCAAACGCAATCTCCCGCACCACAGTTCCGACGTCCGCGCTGAAGGCGGCCATTTTCCGCGCGAGG
CCGATCTGCGCGCGGCCGCCGAGATCCTCAATCAAGGCAAGAAGACCGTCATCCTCGCCGGCGCCGGAGCGCTGCGTGCC
ACCGACGAATTGGAATCGGCCGCGGAGCTTCTCGGCGCGCCCATCGTGAAAGCGCTGCTCGGCAAAGCGGCTGTGCCCGA
CAACAGTCCGTATACTACCGGCGGCATAGGCCTGCTCGGCACCAAGCCCTCGCAGGAGGCTATGGAAAGCTGCGACACCT
TGCTCATGGCGGGAACGTCTTTCCCGTACATCGAGTTCTACCCCAAGCCCGGCAGCGCGCGCGCCGTGCAGATCGAACTC
GACCCCAAACGCATCGGCCTGCGCTACCCGGTGGAGGTGGGGCTCGTCGGCGATTGCAAGCGCACCCTGCAGGCGTTGAT
GCCGATGCTGCGCCGCCGCGAAGACCGCAGCTTCCTGGAGAAGGCGCAGGAGGGTATGCGCGATTGGTGGAAGCTGATGG
AACAGCGCGGCACCCGCACCGATAAGCCGATGAAGCCGCAGGTGGTGGCCTGGGAACTCGGCAAGCGGCTCTCGGAAAAC
GCCATCGTCTCCTCCGATTCCGGCACCAACACTACATGGTGGGCGCGTCAGATTCCCGCCAAGCGCGGCCAGATGCACTC
CTGCTCCGGCAACCTGGCCACCATGGCGCCCGGACTGCCCTACACCATCGCCGCGCAGATCGCGTGGCCTCAGCGTCAGT
GCGTCGCGTTCGTGGGCGATGGCGGCATGTCGATGCTGATGGCCGATTTCGCAACCGCCGTAAAGTATCAACTGCCCATC
AAAGTTGTGGTGGTCAAGAACAACACGCTCGGCCAGATCAAGTGGGAGCAAATGGTCTTCCTCGGCAACCCGGAGTACGG
GGTTGAGCTGCAGCCCATCGATTTCGCCATGTTCGCCCGTGCCTGCGGCGCGCGCGGCTTCACCATCGAGGATCCCGCCG
ATTGCGGCCGCATTCTCGACGAGGCGCTCGCCGTCGATGGCCCCGTCATCATCGAAGCCCTGGTGGATCCCAACGAGCCG
CCTCTGCCCGCCAACATCACTGCCGAGCAGGCCGCCAAGTTCGCCGAGTCCCTGCTGCGCGGCGAACCCAATCGCAAAAA
AATCGCCCTTACCCTCCTGGAGGACCGCGTCAAAGAACTGGTGTAA

Upstream 100 bases:

>100_bases
CTTAATACGGTGCAAACCCTACGGTCGAAGTGGAGTTGCGGGCGGCAAATTGACGCTCGCTCCAGGCTTTCCGTGAGACC
GCCCACGTAGTCCTGCCCGA

Downstream 100 bases:

>100_bases
GTCATCAACGCTGTACTGGTGTGCATTTGTCTGCTCGCAGCCCGCAAATAATCCGCCCCATCAGAGCCGCGACCGTAAAG
TAAGGGAGCGTCCGTTGTAC

Product: pyruvate oxidase

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MCLTVQSRLAPAVYDRGMALTGGDVLVERIIDWGVEVVFGLPGDGINGIMEALRTRQEKIRFIQVRHEESAAFMACAYAK
YTGRLGVCLATSGPGGIHLLNGLYDAKMDSQPVLAITGMHFHDLIGTLAQQDVELDKLFQDVAAFNQRVMSPDHVEGLAD
LACRTAIDYRQVAHITFPTDLQDKEVSKKERSKRNLPHHSSDVRAEGGHFPREADLRAAAEILNQGKKTVILAGAGALRA
TDELESAAELLGAPIVKALLGKAAVPDNSPYTTGGIGLLGTKPSQEAMESCDTLLMAGTSFPYIEFYPKPGSARAVQIEL
DPKRIGLRYPVEVGLVGDCKRTLQALMPMLRRREDRSFLEKAQEGMRDWWKLMEQRGTRTDKPMKPQVVAWELGKRLSEN
AIVSSDSGTNTTWWARQIPAKRGQMHSCSGNLATMAPGLPYTIAAQIAWPQRQCVAFVGDGGMSMLMADFATAVKYQLPI
KVVVVKNNTLGQIKWEQMVFLGNPEYGVELQPIDFAMFARACGARGFTIEDPADCGRILDEALAVDGPVIIEALVDPNEP
PLPANITAEQAAKFAESLLRGEPNRKKIALTLLEDRVKELV

Sequences:

>Translated_601_residues
MCLTVQSRLAPAVYDRGMALTGGDVLVERIIDWGVEVVFGLPGDGINGIMEALRTRQEKIRFIQVRHEESAAFMACAYAK
YTGRLGVCLATSGPGGIHLLNGLYDAKMDSQPVLAITGMHFHDLIGTLAQQDVELDKLFQDVAAFNQRVMSPDHVEGLAD
LACRTAIDYRQVAHITFPTDLQDKEVSKKERSKRNLPHHSSDVRAEGGHFPREADLRAAAEILNQGKKTVILAGAGALRA
TDELESAAELLGAPIVKALLGKAAVPDNSPYTTGGIGLLGTKPSQEAMESCDTLLMAGTSFPYIEFYPKPGSARAVQIEL
DPKRIGLRYPVEVGLVGDCKRTLQALMPMLRRREDRSFLEKAQEGMRDWWKLMEQRGTRTDKPMKPQVVAWELGKRLSEN
AIVSSDSGTNTTWWARQIPAKRGQMHSCSGNLATMAPGLPYTIAAQIAWPQRQCVAFVGDGGMSMLMADFATAVKYQLPI
KVVVVKNNTLGQIKWEQMVFLGNPEYGVELQPIDFAMFARACGARGFTIEDPADCGRILDEALAVDGPVIIEALVDPNEP
PLPANITAEQAAKFAESLLRGEPNRKKIALTLLEDRVKELV
>Mature_601_residues
MCLTVQSRLAPAVYDRGMALTGGDVLVERIIDWGVEVVFGLPGDGINGIMEALRTRQEKIRFIQVRHEESAAFMACAYAK
YTGRLGVCLATSGPGGIHLLNGLYDAKMDSQPVLAITGMHFHDLIGTLAQQDVELDKLFQDVAAFNQRVMSPDHVEGLAD
LACRTAIDYRQVAHITFPTDLQDKEVSKKERSKRNLPHHSSDVRAEGGHFPREADLRAAAEILNQGKKTVILAGAGALRA
TDELESAAELLGAPIVKALLGKAAVPDNSPYTTGGIGLLGTKPSQEAMESCDTLLMAGTSFPYIEFYPKPGSARAVQIEL
DPKRIGLRYPVEVGLVGDCKRTLQALMPMLRRREDRSFLEKAQEGMRDWWKLMEQRGTRTDKPMKPQVVAWELGKRLSEN
AIVSSDSGTNTTWWARQIPAKRGQMHSCSGNLATMAPGLPYTIAAQIAWPQRQCVAFVGDGGMSMLMADFATAVKYQLPI
KVVVVKNNTLGQIKWEQMVFLGNPEYGVELQPIDFAMFARACGARGFTIEDPADCGRILDEALAVDGPVIIEALVDPNEP
PLPANITAEQAAKFAESLLRGEPNRKKIALTLLEDRVKELV

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=23.5602094240838, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=36.0146252285192, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=28.2186948853616, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=25.5434782608696, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=25.4480286738351, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=22.5978647686833, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=579, Percent_Identity=22.9706390328152, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=579, Percent_Identity=22.9706390328152, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=25.6410256410256, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6320816, Length=573, Percent_Identity=21.8150087260035, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922626, Length=494, Percent_Identity=25.1012145748988, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65748; Mature: 65748

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLTVQSRLAPAVYDRGMALTGGDVLVERIIDWGVEVVFGLPGDGINGIMEALRTRQEKI
CEEECHHHCCHHHHHCCCEEECHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
RFIQVRHEESAAFMACAYAKYTGRLGVCLATSGPGGIHLLNGLYDAKMDSQPVLAITGMH
HEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCH
FHDLIGTLAQQDVELDKLFQDVAAFNQRVMSPDHVEGLADLACRTAIDYRQVAHITFPTD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCC
LQDKEVSKKERSKRNLPHHSSDVRAEGGHFPREADLRAAAEILNQGKKTVILAGAGALRA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
TDELESAAELLGAPIVKALLGKAAVPDNSPYTTGGIGLLGTKPSQEAMESCDTLLMAGTS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEECCCC
FPYIEFYPKPGSARAVQIELDPKRIGLRYPVEVGLVGDCKRTLQALMPMLRRREDRSFLE
CCEEEEECCCCCCEEEEEEECHHHCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KAQEGMRDWWKLMEQRGTRTDKPMKPQVVAWELGKRLSENAIVSSDSGTNTTWWARQIPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEHHHCCH
KRGQMHSCSGNLATMAPGLPYTIAAQIAWPQRQCVAFVGDGGMSMLMADFATAVKYQLPI
HCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHEEECCE
KVVVVKNNTLGQIKWEQMVFLGNPEYGVELQPIDFAMFARACGARGFTIEDPADCGRILD
EEEEEECCCCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
EALAVDGPVIIEALVDPNEPPLPANITAEQAAKFAESLLRGEPNRKKIALTLLEDRVKEL
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
V
C
>Mature Secondary Structure
MCLTVQSRLAPAVYDRGMALTGGDVLVERIIDWGVEVVFGLPGDGINGIMEALRTRQEKI
CEEECHHHCCHHHHHCCCEEECHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
RFIQVRHEESAAFMACAYAKYTGRLGVCLATSGPGGIHLLNGLYDAKMDSQPVLAITGMH
HEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCH
FHDLIGTLAQQDVELDKLFQDVAAFNQRVMSPDHVEGLADLACRTAIDYRQVAHITFPTD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCC
LQDKEVSKKERSKRNLPHHSSDVRAEGGHFPREADLRAAAEILNQGKKTVILAGAGALRA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
TDELESAAELLGAPIVKALLGKAAVPDNSPYTTGGIGLLGTKPSQEAMESCDTLLMAGTS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEECCCC
FPYIEFYPKPGSARAVQIELDPKRIGLRYPVEVGLVGDCKRTLQALMPMLRRREDRSFLE
CCEEEEECCCCCCEEEEEEECHHHCCEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KAQEGMRDWWKLMEQRGTRTDKPMKPQVVAWELGKRLSENAIVSSDSGTNTTWWARQIPA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEHHHCCH
KRGQMHSCSGNLATMAPGLPYTIAAQIAWPQRQCVAFVGDGGMSMLMADFATAVKYQLPI
HCCCCCCCCCCCEECCCCCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHEEECCE
KVVVVKNNTLGQIKWEQMVFLGNPEYGVELQPIDFAMFARACGARGFTIEDPADCGRILD
EEEEEECCCCCCEEEEEEEEECCCCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHH
EALAVDGPVIIEALVDPNEPPLPANITAEQAAKFAESLLRGEPNRKKIALTLLEDRVKEL
HHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]