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The map label for this gene is dapE [H]

Identifier: 116626255

GI number: 116626255

Start: 9065614

End: 9066975

Strand: Direct

Name: dapE [H]

Synonym: Acid_7215

Alternate gene names: 116626255

Gene position: 9065614-9066975 (Clockwise)

Preceding gene: 116626250

Following gene: 116626257

Centisome position: 90.97

GC content: 62.92

Gene sequence:

>1362_bases
ATGAACTACTACGAGCAGCATCAAGCCGACTTCCTGGAAGGGCTCAAGACCTTCCTCCGCATTCCGAGCATCAGCACCCT
TTCGGAGAACAAACCCGACATGCGCCGCGCCGCGGAATTCGCGGTCAACGAGCTACGCGCGGCGGGGATGACCGTTGCCG
AGTTGATCGAAGGTGAAGGCGAGAGCAATCCACTGGTTTATGCGGAATGGCTCGGTGCTCCGGGCAAGCCCACCATCCTC
TTTTACGGGCACTACGACGTGCAGCCGCCCGACCCGCTGGACGAATGGAAATCGCCTCCCTTCGAACCTGAAGTGCGCGG
CGACAACATCTATGCGCGTGGCGCCTGTGACGATAAGGGCCAGGTCTACATCCAGATCAAGGCCGTCGAAGGTCTCCTCA
AGACCGCGGGCAAGCTGCCAGTCAACGTCAAGTTCCTGCTCGAAGGCGAGGAAGAGACGGGCGGCGAGCACATCGAGGCG
TACGTCAAGACCAGACCGCCGCGCCTGAAGGCAGATGCCGCGGTGGTCTGCGATACCGAGATGTTCGCTCCCGAGCTGCC
CACCATCTGCGTGGGCCTGCGCGGCATGGTCTACTACGAACTCTTCGTGCAGGGCGCGGATCACGATCTGCACTCCGGCG
TTTATGGAGGCGCGGCGCCCAACCCGATTCTCGCCATCGCGGAGATTCTCTGCGCGCTGAAGGATCGCGCAGGCCGCATC
CAGGTCCCGGGATTTTACGATTCCGTGGAACCTCCGAGCGCCAAGGAGCGCGCGGCATGGGCGAGCCTGCCGTTCGACGA
GAAGGAGTACACCGAAAAGGAAATGGGCGCGAAGGAACTGGTCGGCGAACCCGGCGTGCCGCTCTTCGAGCGCGTGTGGG
CGCGTCCCACGGTGGAGATCCACGGTATTCGCGGCGGGTTCACGGGAGAAGGCGCCAAGACCGTGATTCCGGCACGCGCG
GTGGCCAAGATCAGCACACGCCTGGTGGCCAGCCAGACCGTGGACGCATCGGTCAAGTTGATGCAAGCGGCCGTGAAGGC
GGCATGCCCGCGCGGCGTGACTGCGGAACTCAAGGTGCTCCACGCCGGCGAGCCTTCGTTGACCAACCCCGACAACCCGT
TCATCCATGCTGCCGCCGAAGCCCTCAAGGAAACCTTCGGCAAAGAGACGGTCTACATCCGGTCCGGCGGATCGATCCCG
ATCGTCGGCGTCTTCGACCGCTATCTCGGAATTCCCAGCGTAATGATGGGCTTCGGCTTGCCCGACGACAACCTGCACGC
CCCCAACGAGAAGTTCCATCTCCCGAACTTCTACCGCGGCATCCAGGCCGTGGCACGTTACTTGGAAATCCTCGGACGCT
AA

Upstream 100 bases:

>100_bases
CAGCCGGCTTTCAGCCGGCTCGACCGCTGCATCGCGGCTGCACCCAAGGGTTGAGAACAGACGCCACACTAGCTTCGTGT
TACTAATACGGAAAGCCGCC

Downstream 100 bases:

>100_bases
TATTGCAATCTGCAAATCACGCAAACATGAAAAATATCTTTGCGTTGTTATCAGTAACTTAGCGGACTTACCCACCCTAT
ACTCCGAAACCGGTGTGCTA

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNYYEQHQADFLEGLKTFLRIPSISTLSENKPDMRRAAEFAVNELRAAGMTVAELIEGEGESNPLVYAEWLGAPGKPTIL
FYGHYDVQPPDPLDEWKSPPFEPEVRGDNIYARGACDDKGQVYIQIKAVEGLLKTAGKLPVNVKFLLEGEEETGGEHIEA
YVKTRPPRLKADAAVVCDTEMFAPELPTICVGLRGMVYYELFVQGADHDLHSGVYGGAAPNPILAIAEILCALKDRAGRI
QVPGFYDSVEPPSAKERAAWASLPFDEKEYTEKEMGAKELVGEPGVPLFERVWARPTVEIHGIRGGFTGEGAKTVIPARA
VAKISTRLVASQTVDASVKLMQAAVKAACPRGVTAELKVLHAGEPSLTNPDNPFIHAAAEALKETFGKETVYIRSGGSIP
IVGVFDRYLGIPSVMMGFGLPDDNLHAPNEKFHLPNFYRGIQAVARYLEILGR

Sequences:

>Translated_453_residues
MNYYEQHQADFLEGLKTFLRIPSISTLSENKPDMRRAAEFAVNELRAAGMTVAELIEGEGESNPLVYAEWLGAPGKPTIL
FYGHYDVQPPDPLDEWKSPPFEPEVRGDNIYARGACDDKGQVYIQIKAVEGLLKTAGKLPVNVKFLLEGEEETGGEHIEA
YVKTRPPRLKADAAVVCDTEMFAPELPTICVGLRGMVYYELFVQGADHDLHSGVYGGAAPNPILAIAEILCALKDRAGRI
QVPGFYDSVEPPSAKERAAWASLPFDEKEYTEKEMGAKELVGEPGVPLFERVWARPTVEIHGIRGGFTGEGAKTVIPARA
VAKISTRLVASQTVDASVKLMQAAVKAACPRGVTAELKVLHAGEPSLTNPDNPFIHAAAEALKETFGKETVYIRSGGSIP
IVGVFDRYLGIPSVMMGFGLPDDNLHAPNEKFHLPNFYRGIQAVARYLEILGR
>Mature_453_residues
MNYYEQHQADFLEGLKTFLRIPSISTLSENKPDMRRAAEFAVNELRAAGMTVAELIEGEGESNPLVYAEWLGAPGKPTIL
FYGHYDVQPPDPLDEWKSPPFEPEVRGDNIYARGACDDKGQVYIQIKAVEGLLKTAGKLPVNVKFLLEGEEETGGEHIEA
YVKTRPPRLKADAAVVCDTEMFAPELPTICVGLRGMVYYELFVQGADHDLHSGVYGGAAPNPILAIAEILCALKDRAGRI
QVPGFYDSVEPPSAKERAAWASLPFDEKEYTEKEMGAKELVGEPGVPLFERVWARPTVEIHGIRGGFTGEGAKTVIPARA
VAKISTRLVASQTVDASVKLMQAAVKAACPRGVTAELKVLHAGEPSLTNPDNPFIHAAAEALKETFGKETVYIRSGGSIP
IVGVFDRYLGIPSVMMGFGLPDDNLHAPNEKFHLPNFYRGIQAVARYLEILGR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=462, Percent_Identity=33.3333333333333, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI21071039, Length=464, Percent_Identity=32.5431034482759, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI271398277, Length=316, Percent_Identity=33.2278481012658, Blast_Score=179, Evalue=7e-45,
Organism=Escherichia coli, GI1788816, Length=146, Percent_Identity=28.7671232876712, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17563146, Length=468, Percent_Identity=32.2649572649573, Blast_Score=225, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI193211059, Length=458, Percent_Identity=30.5676855895196, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321159, Length=459, Percent_Identity=29.1938997821351, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6319758, Length=466, Percent_Identity=24.6781115879828, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24585880, Length=469, Percent_Identity=33.9019189765458, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49447; Mature: 49447

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYYEQHQADFLEGLKTFLRIPSISTLSENKPDMRRAAEFAVNELRAAGMTVAELIEGEG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCC
ESNPLVYAEWLGAPGKPTILFYGHYDVQPPDPLDEWKSPPFEPEVRGDNIYARGACDDKG
CCCCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCC
QVYIQIKAVEGLLKTAGKLPVNVKFLLEGEEETGGEHIEAYVKTRPPRLKADAAVVCDTE
EEEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCCCHHEEEHHHCCCCCCCCCEEEEECCH
MFAPELPTICVGLRGMVYYELFVQGADHDLHSGVYGGAAPNPILAIAEILCALKDRAGRI
HHCCCCCHHHHHHHHHHEEEHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
QVPGFYDSVEPPSAKERAAWASLPFDEKEYTEKEMGAKELVGEPGVPLFERVWARPTVEI
ECCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHHCCCCEEE
HGIRGGFTGEGAKTVIPARAVAKISTRLVASQTVDASVKLMQAAVKAACPRGVTAELKVL
EECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HAGEPSLTNPDNPFIHAAAEALKETFGKETVYIRSGGSIPIVGVFDRYLGIPSVMMGFGL
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHCCHHHHHHCCC
PDDNLHAPNEKFHLPNFYRGIQAVARYLEILGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYYEQHQADFLEGLKTFLRIPSISTLSENKPDMRRAAEFAVNELRAAGMTVAELIEGEG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCC
ESNPLVYAEWLGAPGKPTILFYGHYDVQPPDPLDEWKSPPFEPEVRGDNIYARGACDDKG
CCCCEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCEEEECCCCCCC
QVYIQIKAVEGLLKTAGKLPVNVKFLLEGEEETGGEHIEAYVKTRPPRLKADAAVVCDTE
EEEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCCCHHEEEHHHCCCCCCCCCEEEEECCH
MFAPELPTICVGLRGMVYYELFVQGADHDLHSGVYGGAAPNPILAIAEILCALKDRAGRI
HHCCCCCHHHHHHHHHHEEEHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
QVPGFYDSVEPPSAKERAAWASLPFDEKEYTEKEMGAKELVGEPGVPLFERVWARPTVEI
ECCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHHCCCCEEE
HGIRGGFTGEGAKTVIPARAVAKISTRLVASQTVDASVKLMQAAVKAACPRGVTAELKVL
EECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HAGEPSLTNPDNPFIHAAAEALKETFGKETVYIRSGGSIPIVGVFDRYLGIPSVMMGFGL
ECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHCCHHHHHHCCC
PDDNLHAPNEKFHLPNFYRGIQAVARYLEILGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA