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The map label for this gene is cobB [H]

Identifier: 116626098

GI number: 116626098

Start: 8873532

End: 8874218

Strand: Reverse

Name: cobB [H]

Synonym: Acid_7056

Alternate gene names: 116626098

Gene position: 8874218-8873532 (Counterclockwise)

Preceding gene: 116626099

Following gene: 116626093

Centisome position: 89.05

GC content: 65.79

Gene sequence:

>687_bases
GTGGCAAATGCCGCTAGCCTCGCCGTGCTCACCGGCGCTGGAATTTCAGCCGAAAGCGGCATACCTACCTTCCGCGGTGA
CGACGGCTTGTGGAAGAATTACAAGCCGGAAGAACTCGCCACGCCCGAAGCTTTCCTGAAAGATCCGCGGCTGGTCTGGG
AGTGGTACAACTGGCGGCGCGAATTGATCGCCAAGGCGCTGCCCAACGCCGCCCACAAGGCGCTCGTCCAGCTCGAAATC
AAGAAGCGCGAGTACACTCTCATTACCCAGAACGTAGACGGTCTGCACGATCGCGCAGGCAGCGGACGCCAGCTCAAACT
GCACGGCGATATCTGGCGCATGCGCTGCACGGTCTGCGGCGCCAACTGGCCCGACCGCCGCGCGCCGCTCCCCAAGCTGC
CGCCTCACTGCGCCTGCGGCGGCCTCGCGCGGCCCGGCGTCGTCTGGTTCGGCGAAGCGCTGCCGGACGGCATGATGAAA
GAGGCCGAGCACGCCGTCGCCTCCGCCGAAGTCTTCCTGGTGATCGGCACGTCGGCGATGGTCTTCCCCGCCGCCAGCCT
GGTGCCTTACGCCAAACAGGGCGGCGCTAAAGTGATCGAGATCAATACCGCTGAGACACCGTTCACCAACCTCGTGGACT
GCGCCCTGCGCGGGCCCGCGGGCGAACTGCTGCCTGCCCTGCTCTGA

Upstream 100 bases:

>100_bases
TGCGCTTATCTGCGGCCCATTCTGCTTCAAGAAGACCGCGGAAAAAGCGTGGCCCGTTTAAAATAAAATCTTGGCAGACC
TCGATCAAGCTCGTGAATGG

Downstream 100 bases:

>100_bases
CGGCGCTTACTTCGCCGGCGGCTCAAGCGCGAGTTCGACCACTTCCTCCATCGCCTTCACATAGTGGAGCTGGATATCGT
CGATCTGCTCGCTCTTCAGA

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MANAASLAVLTGAGISAESGIPTFRGDDGLWKNYKPEELATPEAFLKDPRLVWEWYNWRRELIAKALPNAAHKALVQLEI
KKREYTLITQNVDGLHDRAGSGRQLKLHGDIWRMRCTVCGANWPDRRAPLPKLPPHCACGGLARPGVVWFGEALPDGMMK
EAEHAVASAEVFLVIGTSAMVFPAASLVPYAKQGGAKVIEINTAETPFTNLVDCALRGPAGELLPALL

Sequences:

>Translated_228_residues
MANAASLAVLTGAGISAESGIPTFRGDDGLWKNYKPEELATPEAFLKDPRLVWEWYNWRRELIAKALPNAAHKALVQLEI
KKREYTLITQNVDGLHDRAGSGRQLKLHGDIWRMRCTVCGANWPDRRAPLPKLPPHCACGGLARPGVVWFGEALPDGMMK
EAEHAVASAEVFLVIGTSAMVFPAASLVPYAKQGGAKVIEINTAETPFTNLVDCALRGPAGELLPALL
>Mature_227_residues
ANAASLAVLTGAGISAESGIPTFRGDDGLWKNYKPEELATPEAFLKDPRLVWEWYNWRRELIAKALPNAAHKALVQLEIK
KREYTLITQNVDGLHDRAGSGRQLKLHGDIWRMRCTVCGANWPDRRAPLPKLPPHCACGGLARPGVVWFGEALPDGMMKE
AEHAVASAEVFLVIGTSAMVFPAASLVPYAKQGGAKVIEINTAETPFTNLVDCALRGPAGELLPALL

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=41.3502109704641, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI6912664, Length=255, Percent_Identity=39.2156862745098, Blast_Score=194, Evalue=5e-50,
Organism=Homo sapiens, GI13787215, Length=237, Percent_Identity=39.662447257384, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI6912662, Length=261, Percent_Identity=28.3524904214559, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI63054862, Length=191, Percent_Identity=35.0785340314136, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI6912660, Length=195, Percent_Identity=34.8717948717949, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300797577, Length=241, Percent_Identity=28.6307053941909, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI13775602, Length=198, Percent_Identity=30.8080808080808, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI13775600, Length=198, Percent_Identity=30.8080808080808, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI7657575, Length=219, Percent_Identity=28.7671232876712, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=42.5438596491228, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17541892, Length=229, Percent_Identity=31.4410480349345, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17567771, Length=240, Percent_Identity=32.0833333333333, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=263, Percent_Identity=28.1368821292776, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71990487, Length=265, Percent_Identity=27.9245283018868, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=213, Percent_Identity=30.5164319248826, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571445, Length=262, Percent_Identity=28.6259541984733, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648389, Length=196, Percent_Identity=30.6122448979592, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137536, Length=221, Percent_Identity=28.5067873303167, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 24717; Mature: 24586

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAASLAVLTGAGISAESGIPTFRGDDGLWKNYKPEELATPEAFLKDPRLVWEWYNWRR
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCHHHHHHHHHHH
ELIAKALPNAAHKALVQLEIKKREYTLITQNVDGLHDRAGSGRQLKLHGDIWRMRCTVCG
HHHHHHCCHHHHHEEEEEEEECCEEEEEECCCCCHHHCCCCCCEEEEECCEEEEEEEEEC
ANWPDRRAPLPKLPPHCACGGLARPGVVWFGEALPDGMMKEAEHAVASAEVFLVIGTSAM
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHH
VFPAASLVPYAKQGGAKVIEINTAETPFTNLVDCALRGPAGELLPALL
HHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
ANAASLAVLTGAGISAESGIPTFRGDDGLWKNYKPEELATPEAFLKDPRLVWEWYNWRR
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCHHHHHHHHHHH
ELIAKALPNAAHKALVQLEIKKREYTLITQNVDGLHDRAGSGRQLKLHGDIWRMRCTVCG
HHHHHHCCHHHHHEEEEEEEECCEEEEEECCCCCHHHCCCCCCEEEEECCEEEEEEEEEC
ANWPDRRAPLPKLPPHCACGGLARPGVVWFGEALPDGMMKEAEHAVASAEVFLVIGTSAM
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHH
VFPAASLVPYAKQGGAKVIEINTAETPFTNLVDCALRGPAGELLPALL
HHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]