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The map label for this gene is gatA [H]

Identifier: 116626070

GI number: 116626070

Start: 8839227

End: 8840609

Strand: Reverse

Name: gatA [H]

Synonym: Acid_7028

Alternate gene names: 116626070

Gene position: 8840609-8839227 (Counterclockwise)

Preceding gene: 116626071

Following gene: 116626069

Centisome position: 88.71

GC content: 65.73

Gene sequence:

>1383_bases
GTGACCATTCTCAACGCCGCCGAAGCCCTGCGATCGCGACGCGTTTCCAGCGTGGAGCTGACCACCGCGGCCATTGCCCG
CATGGATCGCCACAATGATCTGCGCGCCTTCATCACCGCCACCACCGAACAGGCGATGCACCAGGCGCGCACCGCCGATG
CCGAACTGGTCGGCGGAACCGACCGTGGCCCGCTCCACGGCATTCCGATAGCGCTGAAAGATCTCTTCGCCACAAAGGGC
GTCCGCACCACCGCCGGCTCCAGGATCTACGAGCGGTTTATCCCCTCGATCGATGCCACCGTAGTGGCCCGGCTGCACGA
CGCGGGCGCGGTGATGCTGGGTAAGCTCAATATGCACGAGCTGGCCTACGGCATCACTTCGGCCAATCCTCACTTCGGTG
CGGTGCGCAATCCCTGGAACCGGGAGCACAGCCCGGGCGGCTCCAGCGGCGGTTCCGGCGCGGCGGTTGCTTCCGGCATG
GTCTATATGGCGCTCGGCAGCGACACGGGAGGCTCGATCCGTATTCCAGCGTCGTTCTGCGGAACCGTCGGCCTGAAACC
GACCTATGGACGAGTGAGCCGTTATGGTGTGCTGCCGCTCGGCTTCAGCCTCGATCACGTGGGGCCGCTCACGCGCTCGG
TGCGCGACGCCGCGACCGTGCTGAACGCGATCGCCGGCCGCGATCCCCGCGACCCGACTACTTCCCGCCATCCGGTGGTG
GACTATGTCCCCGACGAGCAGTGCTCCATCCGCGGACTGCGCATCGGCTTTCCCGAGAACTTCTACTTCGACCGGCTCGA
CCCGGATGTCGAATCCTCGGTGCGCGGCGCCATCGCTCGCGCCGAATCGCTCGGCGCCCAGGTTCTTCCGGTGGTTCTGC
CGGACATTGCCGCAATGAATGCCGTGGCGCGCATCATCCTGCTCGCCGAAGCCGCTGCCGCCGTCGAACCGCACACGGCC
GACCGCTCGCTTTTCGGACCCGACGTCCTGGCGCTGCTCGACCAGGGTCGTCTTATTCCCGCCACGGACTATGTCAACGC
GCAGCGCCTTCGCCGGAAAATGCGCCGCGAGTTCGACCGGGTCTGGAGTGAAGTGGAGTGCATCATCGCGCCGTCCACTC
CGAACACCGCGCCGCGCATCGGGGACGCGACCATCAGGCTCGGTGGGGTTGAAGAGGATGTGCGTTTAGCCACCACTCGG
CTCGTGCGCGGCATCAACTTGTTGGGTTATCCGTCACTTTCTCTCCCGTGCGGACTCAGCAGTGCGGGCCTTCCCCTCAG
CTTGCAGATCATAGGTCCGGCTTTCGAGGAAGCGCTTCTGCTCCGCGCCGGAGCCGCGTTGGAAGATGCGGGCATAGGCA
TTCCCCCGTGTCCGATGGGTTAA

Upstream 100 bases:

>100_bases
CCCCGCTGGCGGCGCTGGAAGAGACCTTCCGACCTTTGATCCTGGAGCTCACGCCGGATCTCGAACCCGCCCTGGAGCTG
CATCTTGACGGGGATGCCGA

Downstream 100 bases:

>100_bases
TTGCAATTCACCCAATCGAAGGTACTGGCCACTTTGAGTAACCCTCTTCGTGCTGTTTCTCCCTGTATCCGTATTGTTCG
CTCTTAAATGTCATAGGACA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTILNAAEALRSRRVSSVELTTAAIARMDRHNDLRAFITATTEQAMHQARTADAELVGGTDRGPLHGIPIALKDLFATKG
VRTTAGSRIYERFIPSIDATVVARLHDAGAVMLGKLNMHELAYGITSANPHFGAVRNPWNREHSPGGSSGGSGAAVASGM
VYMALGSDTGGSIRIPASFCGTVGLKPTYGRVSRYGVLPLGFSLDHVGPLTRSVRDAATVLNAIAGRDPRDPTTSRHPVV
DYVPDEQCSIRGLRIGFPENFYFDRLDPDVESSVRGAIARAESLGAQVLPVVLPDIAAMNAVARIILLAEAAAAVEPHTA
DRSLFGPDVLALLDQGRLIPATDYVNAQRLRRKMRREFDRVWSEVECIIAPSTPNTAPRIGDATIRLGGVEEDVRLATTR
LVRGINLLGYPSLSLPCGLSSAGLPLSLQIIGPAFEEALLLRAGAALEDAGIGIPPCPMG

Sequences:

>Translated_460_residues
MTILNAAEALRSRRVSSVELTTAAIARMDRHNDLRAFITATTEQAMHQARTADAELVGGTDRGPLHGIPIALKDLFATKG
VRTTAGSRIYERFIPSIDATVVARLHDAGAVMLGKLNMHELAYGITSANPHFGAVRNPWNREHSPGGSSGGSGAAVASGM
VYMALGSDTGGSIRIPASFCGTVGLKPTYGRVSRYGVLPLGFSLDHVGPLTRSVRDAATVLNAIAGRDPRDPTTSRHPVV
DYVPDEQCSIRGLRIGFPENFYFDRLDPDVESSVRGAIARAESLGAQVLPVVLPDIAAMNAVARIILLAEAAAAVEPHTA
DRSLFGPDVLALLDQGRLIPATDYVNAQRLRRKMRREFDRVWSEVECIIAPSTPNTAPRIGDATIRLGGVEEDVRLATTR
LVRGINLLGYPSLSLPCGLSSAGLPLSLQIIGPAFEEALLLRAGAALEDAGIGIPPCPMG
>Mature_459_residues
TILNAAEALRSRRVSSVELTTAAIARMDRHNDLRAFITATTEQAMHQARTADAELVGGTDRGPLHGIPIALKDLFATKGV
RTTAGSRIYERFIPSIDATVVARLHDAGAVMLGKLNMHELAYGITSANPHFGAVRNPWNREHSPGGSSGGSGAAVASGMV
YMALGSDTGGSIRIPASFCGTVGLKPTYGRVSRYGVLPLGFSLDHVGPLTRSVRDAATVLNAIAGRDPRDPTTSRHPVVD
YVPDEQCSIRGLRIGFPENFYFDRLDPDVESSVRGAIARAESLGAQVLPVVLPDIAAMNAVARIILLAEAAAAVEPHTAD
RSLFGPDVLALLDQGRLIPATDYVNAQRLRRKMRREFDRVWSEVECIIAPSTPNTAPRIGDATIRLGGVEEDVRLATTRL
VRGINLLGYPSLSLPCGLSSAGLPLSLQIIGPAFEEALLLRAGAALEDAGIGIPPCPMG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=33.0612244897959, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=28.0241935483871, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI166795287, Length=251, Percent_Identity=33.8645418326693, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=30.6487695749441, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17556264, Length=301, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=315, Percent_Identity=27.3015873015873, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=301, Percent_Identity=30.2325581395349, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556278, Length=300, Percent_Identity=30.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=254, Percent_Identity=29.5275590551181, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=269, Percent_Identity=26.3940520446097, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=268, Percent_Identity=27.9850746268657, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=423, Percent_Identity=26.9503546099291, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=30.2798982188295, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=194, Percent_Identity=33.5051546391753, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=478, Percent_Identity=37.0292887029289, Blast_Score=218, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=23.9130434782609, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=27.1604938271605, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=27.1604938271605, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=27.1604938271605, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=27.1604938271605, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648435, Length=480, Percent_Identity=26.4583333333333, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648437, Length=480, Percent_Identity=26.4583333333333, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=26.4583333333333, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=27.0875763747454, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=26.5979381443299, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=26.6990291262136, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=26.6990291262136, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 48810; Mature: 48679

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILNAAEALRSRRVSSVELTTAAIARMDRHNDLRAFITATTEQAMHQARTADAELVGGT
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEEECCC
DRGPLHGIPIALKDLFATKGVRTTAGSRIYERFIPSIDATVVARLHDAGAVMLGKLNMHE
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCHHH
LAYGITSANPHFGAVRNPWNREHSPGGSSGGSGAAVASGMVYMALGSDTGGSIRIPASFC
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCEEEECHHHH
GTVGLKPTYGRVSRYGVLPLGFSLDHVGPLTRSVRDAATVLNAIAGRDPRDPTTSRHPVV
CCCCCCCCCCCHHHCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
DYVPDEQCSIRGLRIGFPENFYFDRLDPDVESSVRGAIARAESLGAQVLPVVLPDIAAMN
ECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHH
AVARIILLAEAAAAVEPHTADRSLFGPDVLALLDQGRLIPATDYVNAQRLRRKMRREFDR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHH
VWSEVECIIAPSTPNTAPRIGDATIRLGGVEEDVRLATTRLVRGINLLGYPSLSLPCGLS
HHCCEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCC
SAGLPLSLQIIGPAFEEALLLRAGAALEDAGIGIPPCPMG
CCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TILNAAEALRSRRVSSVELTTAAIARMDRHNDLRAFITATTEQAMHQARTADAELVGGT
CCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCEEECCC
DRGPLHGIPIALKDLFATKGVRTTAGSRIYERFIPSIDATVVARLHDAGAVMLGKLNMHE
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCHHH
LAYGITSANPHFGAVRNPWNREHSPGGSSGGSGAAVASGMVYMALGSDTGGSIRIPASFC
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCEEEECHHHH
GTVGLKPTYGRVSRYGVLPLGFSLDHVGPLTRSVRDAATVLNAIAGRDPRDPTTSRHPVV
CCCCCCCCCCCHHHCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
DYVPDEQCSIRGLRIGFPENFYFDRLDPDVESSVRGAIARAESLGAQVLPVVLPDIAAMN
ECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHH
AVARIILLAEAAAAVEPHTADRSLFGPDVLALLDQGRLIPATDYVNAQRLRRKMRREFDR
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHH
VWSEVECIIAPSTPNTAPRIGDATIRLGGVEEDVRLATTRLVRGINLLGYPSLSLPCGLS
HHCCEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCC
SAGLPLSLQIIGPAFEEALLLRAGAALEDAGIGIPPCPMG
CCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA