Click here to switch to the map view.

The map label for this gene is ywlF [H]

Identifier: 116626060

GI number: 116626060

Start: 8821974

End: 8822411

Strand: Reverse

Name: ywlF [H]

Synonym: Acid_7018

Alternate gene names: 116626060

Gene position: 8822411-8821974 (Counterclockwise)

Preceding gene: 116626062

Following gene: 116626059

Centisome position: 88.53

GC content: 59.59

Gene sequence:

>438_bases
ATGAAAATCGCCATTGGCGCGGATCACGCCGGGTTTTCTCTCAAGGAAAAACTGCGGCAGAAACTGGCTCAGGACGGTCA
CGAAGTCGTTGACTTCGGCACCGCATCGGACGCGTCATGCGATTATCCGGATTTCGCCCAACCCGTTGCCCGCGATGTCG
CGCAAGGCCGCAGCGACCGCGGAATTCTCGTCTGCTCCACGGGAATCGGCATGGCCATGGCCGCCAACAAAATCGATGGC
GTCCGCGCCGCTCCCGCGCAGAGCGATGACGAAGTCAAATTCACGCGCGAACACAACGACGCGAACGTGCTCACCCTCGG
CGCCAAGTACCTAGATGAAGATCGCGCCATCGAGCTCATCCACATCTTTCTGAATACGGAATTCGCCGGCGGACGCCATG
CCCGCCGCGTCGCCAAGATCGCCCAGTTGGAAAAATAA

Upstream 100 bases:

>100_bases
GTTTCCGCGTCTGCCGGATTTTCGCGCAGCGTCGGAATGAATAGCCTGCTCCACCGCATAGGCCTGAATTCTAACATGTT
AAAATCATGATTCTCCCCTT

Downstream 100 bases:

>100_bases
GAGGCGGCTTTTCGCCCAGCCTCGGAGGAAAAATCGATATGACCGAATCACAACGCATGTCCCGCACCCTCGCCGAAGTA
GACCCTGAAATTTACCAGGC

Product: ribose-5-phosphate isomerase

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKIAIGADHAGFSLKEKLRQKLAQDGHEVVDFGTASDASCDYPDFAQPVARDVAQGRSDRGILVCSTGIGMAMAANKIDG
VRAAPAQSDDEVKFTREHNDANVLTLGAKYLDEDRAIELIHIFLNTEFAGGRHARRVAKIAQLEK

Sequences:

>Translated_145_residues
MKIAIGADHAGFSLKEKLRQKLAQDGHEVVDFGTASDASCDYPDFAQPVARDVAQGRSDRGILVCSTGIGMAMAANKIDG
VRAAPAQSDDEVKFTREHNDANVLTLGAKYLDEDRAIELIHIFLNTEFAGGRHARRVAKIAQLEK
>Mature_145_residues
MKIAIGADHAGFSLKEKLRQKLAQDGHEVVDFGTASDASCDYPDFAQPVARDVAQGRSDRGILVCSTGIGMAMAANKIDG
VRAAPAQSDDEVKFTREHNDANVLTLGAKYLDEDRAIELIHIFLNTEFAGGRHARRVAKIAQLEK

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=144, Percent_Identity=41.6666666666667, Blast_Score=124, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15708; Mature: 15708

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIGADHAGFSLKEKLRQKLAQDGHEVVDFGTASDASCDYPDFAQPVARDVAQGRSDR
CEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
GILVCSTGIGMAMAANKIDGVRAAPAQSDDEVKFTREHNDANVLTLGAKYLDEDRAIELI
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECHHHCCCCCCEEEE
HIFLNTEFAGGRHARRVAKIAQLEK
EEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIAIGADHAGFSLKEKLRQKLAQDGHEVVDFGTASDASCDYPDFAQPVARDVAQGRSDR
CEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCC
GILVCSTGIGMAMAANKIDGVRAAPAQSDDEVKFTREHNDANVLTLGAKYLDEDRAIELI
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECHHHCCCCCCEEEE
HIFLNTEFAGGRHARRVAKIAQLEK
EEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]