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The map label for this gene is ilvB [H]

Identifier: 116625894

GI number: 116625894

Start: 8590245

End: 8592098

Strand: Direct

Name: ilvB [H]

Synonym: Acid_6848

Alternate gene names: 116625894

Gene position: 8590245-8592098 (Clockwise)

Preceding gene: 116625892

Following gene: 116625895

Centisome position: 86.2

GC content: 62.08

Gene sequence:

>1854_bases
ATGGCGATATCCAAGAAGGCTTCGGTAGATAGACGCGGATTCCTCAGGCGAGCCGCGGGAGGAGCCGCCGCTTTTGTCGC
TGCTCCCATCGTGGGAGAGGCTGCCCCGCAACAGGCCGCTGAAGCCGGACGCGCCGCCGCGATGCCCAACCAGTCCGGAG
TTGCGCGTGAAAGTGGAAATGTACGCCCGGCCCTGAATGTCGAGACAGTCGAGAAACCTGGGTCCGACTACATGGTCGAC
GTCATCAAAGCACTCGGCATCGAATACGTCGCGTTCAACCCCGGTTCGAGCTTCGAGGGGCTGCACGAATCGCTCATTAA
CTACGGCAATAACTCGCCAGAGATTCTGACGTGTACCCACGAAGAGTCCGCTGTCGCCATGGCCCACGGCTATTACAAGA
TCGAAGGCAAGCCGATGCTCGCACTCCTGCACGGGACCATCGGCATTCAGCACGGCTCGATGTCAGTCTACAACGCCTAC
TGCGACGGTGTGCCGGTGTTGATGATCGCCGGATTGGATAACGACGGGGCCGTTGCTGCGCACAACGCCACCGACATGGC
GGCGCCGGTTCGCGACTACGTGAAGTGGGACCACCAACCCGACTCGCTGGCGCAATTCGGACAGTCGCTGGTGCGCGCCT
ACAAACTCGCCATGACGCCTCCCCTGGCTCCTTCCATGCTTGTCATGACAGCACGGCTTCAGAATACCGCCATGACCAAG
GTGCTTCCAGTGCCGAAGTTGGTGATGCCGAGTATCCCCTGCGGCGATCTCGCGTCGGTCCGCGAAGCGGCCAAGATGCT
GGTCGCGGCCGAGAATCCCCGCATCAACGCGGGACGATCCGCGCGCACACAAAGAGGTATGGACCTGCTGGTCCAACTGG
CGGAATTGTTGCAGGCTCCTGTAACCCAAGGCGACCGCGCCGCATTCCCCTCGCGGCATCCTCTTGCCGGAATCGGCTTG
GGCGATCCCGACCTGTTCCTGGCGCTCGAAGGCGATATTCCCGCCTCCCGCGGAAGCGCCAAAGCCATCAGCATCAGCAC
GGCGGAACTGATGGCCACGCAGAACTACAACATCGCCAACAATCCTCCTCAGGCCGATCTCGCCATCACCGGCGACCCCG
AAGCTACGCTGCCCCTGCTGATCGAAGAGGTGAACACGCTAATCACCGCTGACCGCAAGCGGGCCTACGAAGATCGCGGC
AAGAAGTACGCCGCCGCGCACCTGCAGCGCCGTAATGCCTTGATCGATGCGACCGCTCCCGGCTGGAACGCGAGCCCCAT
CAGTCTCGCCCGCCTTTGCGCCGAGCTGTGGCCTCTGATCGAGCACGATGACTGGTCGCTGACTTCGCCGCAGGGCTTCA
TCAGCAACTGGCCCAACATCCTCTGGAACATGGATAAGACTTACCGGACCATCGGCTCGCAGGGCGGAGGCGCGATGGGC
TACGGGGCTCCCGCTTCGGTCGGTGCAGCGTTGGCGAACCGCAGGCACGGGCGTTTGAGTATCAACATCCAGTGCGACGG
CGACTTGAACTACGCGCCCGGGGTGCTGTGGACGGCGGCGCACCATCGCATCCCGCTGCTGACCATCATGCACAACAACC
GCGGCTATCATCAGGAAGTAATGTTCATTCAGCAGCAGTGCAGCATCCGCAACCGCGGCGGCGATAGGGTCTACCTCGGC
ACCAAGCTGTTCGATCCCGACATCGACTACGCGTCGATGGCCAAGGCGTACGGCCTTTTTGGCGAGGGTCCGATCTCCGA
TCCCAAGGACCTGTCACCAGCTCTGAAACGCGGCATCGAACGCGTGAAGAAAGGCGAGCCCGCACTTATCGATGTTGTCA
CGCAGCCGCGCTAA

Upstream 100 bases:

>100_bases
CCGCCGGCCCGGCTGTCCAAGATGGCCAAAAGCGATGCGCAGCGCGTCTTCCGCGGAGGTATACTTATCCGACTAGCGCG
CAGCAGGCAGGAGAACCTTG

Downstream 100 bases:

>100_bases
GGAGACGGACCATGAAGATCGTCACGGTCATCGGAATGATTCTGGCGGGAACCCTCCCGGCTTTGAGCCAGGCTCCAGCC
AGCGACACTACCAAAGGCAA

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MAISKKASVDRRGFLRRAAGGAAAFVAAPIVGEAAPQQAAEAGRAAAMPNQSGVARESGNVRPALNVETVEKPGSDYMVD
VIKALGIEYVAFNPGSSFEGLHESLINYGNNSPEILTCTHEESAVAMAHGYYKIEGKPMLALLHGTIGIQHGSMSVYNAY
CDGVPVLMIAGLDNDGAVAAHNATDMAAPVRDYVKWDHQPDSLAQFGQSLVRAYKLAMTPPLAPSMLVMTARLQNTAMTK
VLPVPKLVMPSIPCGDLASVREAAKMLVAAENPRINAGRSARTQRGMDLLVQLAELLQAPVTQGDRAAFPSRHPLAGIGL
GDPDLFLALEGDIPASRGSAKAISISTAELMATQNYNIANNPPQADLAITGDPEATLPLLIEEVNTLITADRKRAYEDRG
KKYAAAHLQRRNALIDATAPGWNASPISLARLCAELWPLIEHDDWSLTSPQGFISNWPNILWNMDKTYRTIGSQGGGAMG
YGAPASVGAALANRRHGRLSINIQCDGDLNYAPGVLWTAAHHRIPLLTIMHNNRGYHQEVMFIQQQCSIRNRGGDRVYLG
TKLFDPDIDYASMAKAYGLFGEGPISDPKDLSPALKRGIERVKKGEPALIDVVTQPR

Sequences:

>Translated_617_residues
MAISKKASVDRRGFLRRAAGGAAAFVAAPIVGEAAPQQAAEAGRAAAMPNQSGVARESGNVRPALNVETVEKPGSDYMVD
VIKALGIEYVAFNPGSSFEGLHESLINYGNNSPEILTCTHEESAVAMAHGYYKIEGKPMLALLHGTIGIQHGSMSVYNAY
CDGVPVLMIAGLDNDGAVAAHNATDMAAPVRDYVKWDHQPDSLAQFGQSLVRAYKLAMTPPLAPSMLVMTARLQNTAMTK
VLPVPKLVMPSIPCGDLASVREAAKMLVAAENPRINAGRSARTQRGMDLLVQLAELLQAPVTQGDRAAFPSRHPLAGIGL
GDPDLFLALEGDIPASRGSAKAISISTAELMATQNYNIANNPPQADLAITGDPEATLPLLIEEVNTLITADRKRAYEDRG
KKYAAAHLQRRNALIDATAPGWNASPISLARLCAELWPLIEHDDWSLTSPQGFISNWPNILWNMDKTYRTIGSQGGGAMG
YGAPASVGAALANRRHGRLSINIQCDGDLNYAPGVLWTAAHHRIPLLTIMHNNRGYHQEVMFIQQQCSIRNRGGDRVYLG
TKLFDPDIDYASMAKAYGLFGEGPISDPKDLSPALKRGIERVKKGEPALIDVVTQPR
>Mature_616_residues
AISKKASVDRRGFLRRAAGGAAAFVAAPIVGEAAPQQAAEAGRAAAMPNQSGVARESGNVRPALNVETVEKPGSDYMVDV
IKALGIEYVAFNPGSSFEGLHESLINYGNNSPEILTCTHEESAVAMAHGYYKIEGKPMLALLHGTIGIQHGSMSVYNAYC
DGVPVLMIAGLDNDGAVAAHNATDMAAPVRDYVKWDHQPDSLAQFGQSLVRAYKLAMTPPLAPSMLVMTARLQNTAMTKV
LPVPKLVMPSIPCGDLASVREAAKMLVAAENPRINAGRSARTQRGMDLLVQLAELLQAPVTQGDRAAFPSRHPLAGIGLG
DPDLFLALEGDIPASRGSAKAISISTAELMATQNYNIANNPPQADLAITGDPEATLPLLIEEVNTLITADRKRAYEDRGK
KYAAAHLQRRNALIDATAPGWNASPISLARLCAELWPLIEHDDWSLTSPQGFISNWPNILWNMDKTYRTIGSQGGGAMGY
GAPASVGAALANRRHGRLSINIQCDGDLNYAPGVLWTAAHHRIPLLTIMHNNRGYHQEVMFIQQQCSIRNRGGDRVYLGT
KLFDPDIDYASMAKAYGLFGEGPISDPKDLSPALKRGIERVKKGEPALIDVVTQPR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66140; Mature: 66009

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISKKASVDRRGFLRRAAGGAAAFVAAPIVGEAAPQQAAEAGRAAAMPNQSGVARESGN
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCCCCCCC
VRPALNVETVEKPGSDYMVDVIKALGIEYVAFNPGSSFEGLHESLINYGNNSPEILTCTH
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEC
EESAVAMAHGYYKIEGKPMLALLHGTIGIQHGSMSVYNAYCDGVPVLMIAGLDNDGAVAA
CCCEEEEECCEEEECCCEEEEEEECHHEEECCCCEEEHHHCCCCEEEEEEECCCCCCEEE
HNATDMAAPVRDYVKWDHQPDSLAQFGQSLVRAYKLAMTPPLAPSMLVMTARLQNTAMTK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VLPVPKLVMPSIPCGDLASVREAAKMLVAAENPRINAGRSARTQRGMDLLVQLAELLQAP
HCCCCHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTQGDRAAFPSRHPLAGIGLGDPDLFLALEGDIPASRGSAKAISISTAELMATQNYNIAN
CCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHEECCCCCCC
NPPQADLAITGDPEATLPLLIEEVNTLITADRKRAYEDRGKKYAAAHLQRRNALIDATAP
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEECCC
GWNASPISLARLCAELWPLIEHDDWSLTSPQGFISNWPNILWNMDKTYRTIGSQGGGAMG
CCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCHHEECCCHHHHHHCCCCCCCCC
YGAPASVGAALANRRHGRLSINIQCDGDLNYAPGVLWTAAHHRIPLLTIMHNNRGYHQEV
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHH
MFIQQQCSIRNRGGDRVYLGTKLFDPDIDYASMAKAYGLFGEGPISDPKDLSPALKRGIE
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
RVKKGEPALIDVVTQPR
HHHCCCCEEEEEECCCC
>Mature Secondary Structure 
AISKKASVDRRGFLRRAAGGAAAFVAAPIVGEAAPQQAAEAGRAAAMPNQSGVARESGN
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCCCCCCCC
VRPALNVETVEKPGSDYMVDVIKALGIEYVAFNPGSSFEGLHESLINYGNNSPEILTCTH
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEC
EESAVAMAHGYYKIEGKPMLALLHGTIGIQHGSMSVYNAYCDGVPVLMIAGLDNDGAVAA
CCCEEEEECCEEEECCCEEEEEEECHHEEECCCCEEEHHHCCCCEEEEEEECCCCCCEEE
HNATDMAAPVRDYVKWDHQPDSLAQFGQSLVRAYKLAMTPPLAPSMLVMTARLQNTAMTK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
VLPVPKLVMPSIPCGDLASVREAAKMLVAAENPRINAGRSARTQRGMDLLVQLAELLQAP
HCCCCHHHCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTQGDRAAFPSRHPLAGIGLGDPDLFLALEGDIPASRGSAKAISISTAELMATQNYNIAN
CCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHEECCCCCCC
NPPQADLAITGDPEATLPLLIEEVNTLITADRKRAYEDRGKKYAAAHLQRRNALIDATAP
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEECCC
GWNASPISLARLCAELWPLIEHDDWSLTSPQGFISNWPNILWNMDKTYRTIGSQGGGAMG
CCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCHHEECCCHHHHHHCCCCCCCCC
YGAPASVGAALANRRHGRLSINIQCDGDLNYAPGVLWTAAHHRIPLLTIMHNNRGYHQEV
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHH
MFIQQQCSIRNRGGDRVYLGTKLFDPDIDYASMAKAYGLFGEGPISDPKDLSPALKRGIE
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
RVKKGEPALIDVVTQPR
HHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]