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The map label for this gene is istB [H]

Identifier: 116625852

GI number: 116625852

Start: 8535780

End: 8536568

Strand: Reverse

Name: istB [H]

Synonym: Acid_6806

Alternate gene names: 116625852

Gene position: 8536568-8535780 (Counterclockwise)

Preceding gene: 116625853

Following gene: 116625851

Centisome position: 85.66

GC content: 57.16

Gene sequence:

>789_bases
ATGTCGCTGCAATCTGAACGACTGCAACTACTCATGCAACGGCTGAGCCTCAACGAAATGGTGCATGGCTATCAAGCTCT
GGCAGAGCAGGCTGCCCAAAAGAACTTGCCGTACTGCGATTTCCTGGTGCTGGAGAGTGAAGCCCACGTGCGGCAGGAGC
GGAACATCAAAGTCCGCACACAACTGGCGGGCTTTCCGGACACTAAGCGGTTGGAGCAGTTTGACTTCAAGTTCCCGTCC
TCCATCGACGAGAAGAAAATCCGCGAACTAGCGAGCCTGCGTTTTCTGGAGCGCCATGAGAACGCTGTGTTCCTCGGACC
GCCCGGGGTGGGCAAGACCCACCTGGCGGTCTCGTTGGGAATGGAGGCCATTCGTCACGGCTTCAGTGTTTACTTTGTCA
GTTTGACGGATCTGCTCGATCGTCTGGTGCAGGCGCAGGCGGAGAATCGGATGCGCCGCAAGATGGCGCATCTGCGTAAG
TGCCGGCTTTTGATCCTCGATGAGATCGGCTACCGCAGTTTGGATAAACAGGCGACCACCTGTCTGTTCCAGTTGATCAG
CGAACGTTACGAACGCGGCTCGATGATTCTGACCTCCAACAAGAGCTACGGCGAGTGGGGATCGATCTTTCAGGACAACG
TGATCGCCAGCGCGATTCTGGATCGGCTGTTGCACCACTCCCACACCGTCAACATCAAGGGCGAAAGCTACCGGCTGAAG
GAGAAACGCAAGGCCGGCGTATGGACACAGTCCGCCCCCGAATCGGTCAGTTCCGGAGGTGGCCAATGA

Upstream 100 bases:

>100_bases
CCAAACCTGCTGGAACTGGCGCCGGGCCCGGGAGTCGGTCGCGGGTTTCTGGCGCCGGAAGTGGAACAGCGTTCGCTGGA
CGTGTACCAGGAGGTAGCCG

Downstream 100 bases:

>100_bases
TCCCGGCACCGCGTTTGCGATTGAAGAAAGCTACGGGATGGTTCGCCGCCGGGCAGGAGGTCGCCGCCGCTTTGGCGATG
CTCTCCGATGCGGCATTCAA

Product: IstB ATP binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSLQSERLQLLMQRLSLNEMVHGYQALAEQAAQKNLPYCDFLVLESEAHVRQERNIKVRTQLAGFPDTKRLEQFDFKFPS
SIDEKKIRELASLRFLERHENAVFLGPPGVGKTHLAVSLGMEAIRHGFSVYFVSLTDLLDRLVQAQAENRMRRKMAHLRK
CRLLILDEIGYRSLDKQATTCLFQLISERYERGSMILTSNKSYGEWGSIFQDNVIASAILDRLLHHSHTVNIKGESYRLK
EKRKAGVWTQSAPESVSSGGGQ

Sequences:

>Translated_262_residues
MSLQSERLQLLMQRLSLNEMVHGYQALAEQAAQKNLPYCDFLVLESEAHVRQERNIKVRTQLAGFPDTKRLEQFDFKFPS
SIDEKKIRELASLRFLERHENAVFLGPPGVGKTHLAVSLGMEAIRHGFSVYFVSLTDLLDRLVQAQAENRMRRKMAHLRK
CRLLILDEIGYRSLDKQATTCLFQLISERYERGSMILTSNKSYGEWGSIFQDNVIASAILDRLLHHSHTVNIKGESYRLK
EKRKAGVWTQSAPESVSSGGGQ
>Mature_261_residues
SLQSERLQLLMQRLSLNEMVHGYQALAEQAAQKNLPYCDFLVLESEAHVRQERNIKVRTQLAGFPDTKRLEQFDFKFPSS
IDEKKIRELASLRFLERHENAVFLGPPGVGKTHLAVSLGMEAIRHGFSVYFVSLTDLLDRLVQAQAENRMRRKMAHLRKC
RLLILDEIGYRSLDKQATTCLFQLISERYERGSMILTSNKSYGEWGSIFQDNVIASAILDRLLHHSHTVNIKGESYRLKE
KRKAGVWTQSAPESVSSGGGQ

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29950; Mature: 29819

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQSERLQLLMQRLSLNEMVHGYQALAEQAAQKNLPYCDFLVLESEAHVRQERNIKVRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEEE
QLAGFPDTKRLEQFDFKFPSSIDEKKIRELASLRFLERHENAVFLGPPGVGKTHLAVSLG
EECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
MEAIRHGFSVYFVSLTDLLDRLVQAQAENRMRRKMAHLRKCRLLILDEIGYRSLDKQATT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CLFQLISERYERGSMILTSNKSYGEWGSIFQDNVIASAILDRLLHHSHTVNIKGESYRLK
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHH
EKRKAGVWTQSAPESVSSGGGQ
HHHHCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SLQSERLQLLMQRLSLNEMVHGYQALAEQAAQKNLPYCDFLVLESEAHVRQERNIKVRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCEEEE
QLAGFPDTKRLEQFDFKFPSSIDEKKIRELASLRFLERHENAVFLGPPGVGKTHLAVSLG
EECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
MEAIRHGFSVYFVSLTDLLDRLVQAQAENRMRRKMAHLRKCRLLILDEIGYRSLDKQATT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
CLFQLISERYERGSMILTSNKSYGEWGSIFQDNVIASAILDRLLHHSHTVNIKGESYRLK
HHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHH
EKRKAGVWTQSAPESVSSGGGQ
HHHHCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]