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The map label for this gene is xthA [H]

Identifier: 116625780

GI number: 116625780

Start: 8433485

End: 8434261

Strand: Direct

Name: xthA [H]

Synonym: Acid_6730

Alternate gene names: 116625780

Gene position: 8433485-8434261 (Clockwise)

Preceding gene: 116625779

Following gene: 116625782

Centisome position: 84.63

GC content: 61.13

Gene sequence:

>777_bases
GTGAAAATCGCAACTTTTAACATCAACAACATTAATAAGCGCCTGAATAATCTCACGGCGTGGCTAAGCAGGGCGCAGCC
CGACGTCGTCTGCCTGCAGGAGCTCAAAGCGGAGCAGCGGGCATTTCCGGCCAGCGCGCTTCGAGACCTCGGTTATGACG
GATTCTGGCTTGGCGAACGGTCCTGGAATGGCGTCGCGATCTTTGCGCGGGAATGCACACCGGTGCTGACGCGATCCAGC
TTGCCTGGGAATCCCAAGGATAGCCAGGCGCGCTATATCGAGGCGGCGGTCAATGGCGTGCTCATCGCTTCCCTGTACCT
GCCGAACGGTAACCCTCAACCCGGCCCGAAGTTCGATTACAAATTGGCCTGGTTCCAGCGCTTAATCGAACACGGGCAGG
AACTCATGAAGGCCGGCGTGCCCGTCGTACTAGCCGGCGACTACAACGTGGTGCCGACCGCGCAGGACATCTATCCCACG
CGATCTCTCGATAAGAACGCGCTGATTCAACCTCAGAGCCGCCAGGCGTATGCCCGCCTGCTCGCTCAGGGCTGGACGGA
CGCCCTGCGAACGCTACAACCCGAAGGGCCGCTTTGGACGTTTTGGGACTACGAGCGCGGCCGCTGGCCTTTAGGCAAGG
GGATGCGGCTGGATCATTTCCTGCTCTCTCCCACCGTGTCGGACCGGCTCGTGGACGGAGGTGTGGACCGTTGGGTTCGT
GGAGAGGAGAACGCGAGCGACCACGCTCCCGCCTGGATCGTGCTGGATCGGACGTGA

Upstream 100 bases:

>100_bases
TTGGCGGCTACAAGCAAAGCGGCTTCGGCCGCGACCTCGGCAGCTACGCCCTCGATCAATACACCAACGTAAAATCCGTC
TGGATCGCGCTCGGCAAATA

Downstream 100 bases:

>100_bases
TGCTTACCGGATGGGTCGGGCACCGGCGATCAGAATATCCACGAGTCTCCTGGCGCTTTGCTGCCAGTCCGGGCTGGTGG
CGACGCTGGCGACGCCGATT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKIATFNINNINKRLNNLTAWLSRAQPDVVCLQELKAEQRAFPASALRDLGYDGFWLGERSWNGVAIFARECTPVLTRSS
LPGNPKDSQARYIEAAVNGVLIASLYLPNGNPQPGPKFDYKLAWFQRLIEHGQELMKAGVPVVLAGDYNVVPTAQDIYPT
RSLDKNALIQPQSRQAYARLLAQGWTDALRTLQPEGPLWTFWDYERGRWPLGKGMRLDHFLLSPTVSDRLVDGGVDRWVR
GEENASDHAPAWIVLDRT

Sequences:

>Translated_258_residues
MKIATFNINNINKRLNNLTAWLSRAQPDVVCLQELKAEQRAFPASALRDLGYDGFWLGERSWNGVAIFARECTPVLTRSS
LPGNPKDSQARYIEAAVNGVLIASLYLPNGNPQPGPKFDYKLAWFQRLIEHGQELMKAGVPVVLAGDYNVVPTAQDIYPT
RSLDKNALIQPQSRQAYARLLAQGWTDALRTLQPEGPLWTFWDYERGRWPLGKGMRLDHFLLSPTVSDRLVDGGVDRWVR
GEENASDHAPAWIVLDRT
>Mature_258_residues
MKIATFNINNINKRLNNLTAWLSRAQPDVVCLQELKAEQRAFPASALRDLGYDGFWLGERSWNGVAIFARECTPVLTRSS
LPGNPKDSQARYIEAAVNGVLIASLYLPNGNPQPGPKFDYKLAWFQRLIEHGQELMKAGVPVVLAGDYNVVPTAQDIYPT
RSLDKNALIQPQSRQAYARLLAQGWTDALRTLQPEGPLWTFWDYERGRWPLGKGMRLDHFLLSPTVSDRLVDGGVDRWVR
GEENASDHAPAWIVLDRT

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=266, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI18375503, Length=266, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI18375501, Length=266, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=6e-21,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=31.9702602230483, Blast_Score=140, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71989536, Length=246, Percent_Identity=24.7967479674797, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330655, Length=264, Percent_Identity=28.4090909090909, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136678, Length=264, Percent_Identity=28.4090909090909, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29055; Mature: 29055

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNINNINKRLNNLTAWLSRAQPDVVCLQELKAEQRAFPASALRDLGYDGFWLGER
CEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCC
SWNGVAIFARECTPVLTRSSLPGNPKDSQARYIEAAVNGVLIASLYLPNGNPQPGPKFDY
CCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCH
KLAWFQRLIEHGQELMKAGVPVVLAGDYNVVPTAQDIYPTRSLDKNALIQPQSRQAYARL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHH
LAQGWTDALRTLQPEGPLWTFWDYERGRWPLGKGMRLDHFLLSPTVSDRLVDGGVDRWVR
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHCCHHHHCC
GEENASDHAPAWIVLDRT
CCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKIATFNINNINKRLNNLTAWLSRAQPDVVCLQELKAEQRAFPASALRDLGYDGFWLGER
CEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCC
SWNGVAIFARECTPVLTRSSLPGNPKDSQARYIEAAVNGVLIASLYLPNGNPQPGPKFDY
CCCCEEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCH
KLAWFQRLIEHGQELMKAGVPVVLAGDYNVVPTAQDIYPTRSLDKNALIQPQSRQAYARL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHH
LAQGWTDALRTLQPEGPLWTFWDYERGRWPLGKGMRLDHFLLSPTVSDRLVDGGVDRWVR
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHCCHHHHCC
GEENASDHAPAWIVLDRT
CCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]