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The map label for this gene is miaB

Identifier: 116625754

GI number: 116625754

Start: 8405580

End: 8406917

Strand: Direct

Name: miaB

Synonym: Acid_6704

Alternate gene names: 116625754

Gene position: 8405580-8406917 (Clockwise)

Preceding gene: 116625751

Following gene: 116625755

Centisome position: 84.35

GC content: 62.26

Gene sequence:

>1338_bases
ATGGGGGCAGTGTCTGGGAAAACCTTTTACATTGAAACGTTCGGCTGCCAGATGAATGCGCACGACTCCGAGAAAGTCGT
AGGGACGCTACTGGCGGAGGGATACGAGCAGGTGGCGACGCCGGAAGCGGCGGAGCTGGTGTTTTACAACACCTGCAGCA
TTCGGGATAAGGCGGAGCAGAAGGTTTTCAACCGGCTGCAGAACTTCAAGCGCGAGGGGATGAAAGGCAAGATCTTCGGC
GTGCTGGGGTGCGTGGCGCAGCAGGAAGGCGAGAAGATCTTCGACCGTGCGCCGCACGTCTCCCTGGTGGCGGGGTCGGC
GAGCTACACGCGGCTGCCGGAGATGCTGGTGCAACTGGAAGCCGGCAATCGGCGGGTGACGGGGCTGTCGCTCGATACCG
AGGAGACTTTCGACACGCCGTTCACGCGACGCGACAATCCGCATCGGGCGTACCTTACGATCATCGAAGGCTGCGATAAG
GCGTGCGCGTATTGCGTGGTGCCGTTCACGCGGGGGCCGGAGCGGAGCAGGACGAGCGAGAGCGTGATGGCGGAGGCGCG
CGGGCTTACAGAGAAGGGCTACACCGAAGTCCAGTTGCTGGGGCAGAACGTGAACAGCTATCGCGACCCATCGCCCGCCG
GCTGGGATTTCGCGACGCTGCTGGCGAAGGTGGCGGAGATTCCGGGGATGCGGCGGGTGCGGTACACGACCAGCCATCCG
CGCGACTTCGTGAGGCCGATTGTGGACGCGATGGACGCCAACGAGGCGATTTGCGATCACATTCACCTGCCGGTGCAGTC
GGGATCGAGCAAAGTACTGGCGGCGATGGACCGCCTGTACACGCGCGACGAATACCTGCGGCGGATCGATTGGATCAAGA
GCGCGAAGCGGCGGTACTCGCTGACGACGGACATCATCATCGGATTTCCGGGCGAGACCGACGCGGATTTCGAGCAGACT
CTGGACCTGCTGGACGAAGTGCAGTACGACTCGCTGTTCAGCTTCAAGTACTCGCCGCGCCCCAACACTTCGGCGTTGGC
TATGGAAGACCGGATTCCGGAAGAGGAGAAGCAGCGGCGGCTGCTGACCCTGCAGGAGAAGCAGCGGGCGATCCAGATCC
GACGCAACGCGGAGATGATCGGGAGCATTCAGGAAGTGCTGGTGGAAGGTCGCAACCAGGCGTTGGGGCAGTGGATCGGG
CGCACCACCTGTAATCGCACGCTGAATTTTTCTCATCCCGACACAAATGGAAACGAGCTGGTAGGCAAGTACCTCCCGGT
ACGGGTGACACGGTCGGGCCCCAACTCGCTAGTGGGTGAGAGCGCGGCGTTGGTGTAA

Upstream 100 bases:

>100_bases
TCTGCGGCTCCGCGGCTCTGCGAGAAAAAGGAGGCTCGATACGTCGGGAAACCAGCCAGGAAACCAGCCAAAAAACCAGG
ACGAGGGTGGGGCTAGTACA

Downstream 100 bases:

>100_bases
CTGTGGTGTAATCGGAGTGGGGAGGGCCGCAGAAAAAATGGAAGTAGAAATGAAAATTCGCGGGCTCATGATGGATCCCG
TAACCAACATGCCCATCGTC

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQKVFNRLQNFKREGMKGKIFG
VLGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLEAGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDK
ACAYCVVPFTRGPERSRTSESVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHP
RDFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYSLTTDIIIGFPGETDADFEQT
LDLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRRLLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIG
RTTCNRTLNFSHPDTNGNELVGKYLPVRVTRSGPNSLVGESAALV

Sequences:

>Translated_445_residues
MGAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQKVFNRLQNFKREGMKGKIFG
VLGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLEAGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDK
ACAYCVVPFTRGPERSRTSESVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHP
RDFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYSLTTDIIIGFPGETDADFEQT
LDLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRRLLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIG
RTTCNRTLNFSHPDTNGNELVGKYLPVRVTRSGPNSLVGESAALV
>Mature_444_residues
GAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQKVFNRLQNFKREGMKGKIFGV
LGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLEAGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDKA
CAYCVVPFTRGPERSRTSESVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHPR
DFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYSLTTDIIIGFPGETDADFEQTL
DLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRRLLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIGR
TTCNRTLNFSHPDTNGNELVGKYLPVRVTRSGPNSLVGESAALV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=36.7088607594937, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=36.7088607594937, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI93277076, Length=374, Percent_Identity=30.4812834224599, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=41.8552036199095, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787057, Length=387, Percent_Identity=30.4909560723514, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=443, Percent_Identity=38.6004514672686, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI71996771, Length=377, Percent_Identity=26.2599469496021, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356207, Length=486, Percent_Identity=34.7736625514403, Blast_Score=271, Evalue=5e-73,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=29.2035398230088, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SOLUE (Q01RU5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827910.1
- ProteinModelPortal:   Q01RU5
- SMR:   Q01RU5
- STRING:   Q01RU5
- GeneID:   4425174
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6704
- NMPDR:   fig|234267.9.peg.6374
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q01RU5
- ProtClustDB:   CLSK774647
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49971; Mature: 49840

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQ
CCCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHH
KVFNRLQNFKREGMKGKIFGVLGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
AGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDKACAYCVVPFTRGPERSRTSE
CCCEEEEEEECCCHHHCCCCCCCCCCCCCEEEHHHHHCCHHHEEEEEECCCCCCCCCHHH
SVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHP
HHHHHHCCCCCCCCCEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
RDFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYS
HHHHHHHHHHHCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTTDIIIGFPGETDADFEQTLDLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRR
CEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHCCCCHHHHHH
LLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIGRTTCNRTLNFSHPDTNGNEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCCCCHH
VGKYLPVRVTRSGPNSLVGESAALV
HCCCCEEEEECCCCCCHHCCCCCCC
>Mature Secondary Structure 
GAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQ
CCCCCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHH
KVFNRLQNFKREGMKGKIFGVLGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCHHHHHHHHHHHHC
AGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDKACAYCVVPFTRGPERSRTSE
CCCEEEEEEECCCHHHCCCCCCCCCCCCCEEEHHHHHCCHHHEEEEEECCCCCCCCCHHH
SVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHP
HHHHHHCCCCCCCCCEEEEECCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC
RDFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYS
HHHHHHHHHHHCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTTDIIIGFPGETDADFEQTLDLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRR
CEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHHCCCCHHHHHH
LLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIGRTTCNRTLNFSHPDTNGNEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCCCCCCHH
VGKYLPVRVTRSGPNSLVGESAALV
HCCCCEEEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA