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The map label for this gene is psuK [H]

Identifier: 116625710

GI number: 116625710

Start: 8360594

End: 8361493

Strand: Direct

Name: psuK [H]

Synonym: Acid_6659

Alternate gene names: 116625710

Gene position: 8360594-8361493 (Clockwise)

Preceding gene: 116625708

Following gene: 116625711

Centisome position: 83.89

GC content: 66.78

Gene sequence:

>900_bases
GTGAAGATCGCCTGTATCGGCGGCGCCCACATCGATCGCCACGGCCTACTCGGCGGTCCGCTCATCCTCGGTACCTCCAA
CCCCGGCGCCGTCACCAGCGATTTCGGTGGCGTCGCCAGGAACGTCGCCGAGAACCTTTCGCGCCTCGGCCGCGAAGTCC
TCATGGTCTCGCGCGTCGGCGATGACGAGACCGGCCGCGCCGTCGTCTCTCACCTCAACTCGCTCGGCATCGACACCTCC
CCGGTGACCATATCCACTCGCCCCACCGCATCCTATACGGCCATTCTCGAACCCAACGGCGAGCTCGTCCTTGGCCTTGC
CGACATGGACATCTACGACGAAATCACACCTGAACTGCTGGAGCCCTGCCTGCCTCGCCTGCTCGAATGCGAGCTCTGGT
TCATCGACGCCAACCTCTCCGCCGCCACTATCGAGTGGATCTCCCGCGTCTCTGCCCACCTCGCGCTCGCCGCCGATGCC
GTTTCCGTCGTCAAGTCGCGCAAACTCGCCGGCATCCTCGATACAGTCTCGCCGCTCTTCCTGAACATCGCGCAGGCCGC
CTCCCTCCTCGGCGTCGATGCGTTCCCCGATTCCGAGGCCGCCGCGCGGGCTATGTCGCAGCACGTACTCGCCGGCGTGG
TCACCGCCGGAGCCGCGGGTGTGGCCGCGTGGCTCAACAATGAAATCCGTGTCCTCCCCGCGCGCCGCGCTGCCGTACGC
GATGTCACCGGAGCCGGCGACGCCCTCATCGCCGGCACCCTCTTCGGCATCACCGGCGGCGTGCCCGGCTTTTTCCGCGC
CGTGCAGCTCGGTCTGGCCGCCGCTGCAATCACGGTAGAATCGGGATCGACAACGGCCCCGCACCTCACCGCGGACCTGC
TCTATGCATCTCAAGATTGA

Upstream 100 bases:

>100_bases
CGACCCGTGTGGCGCAGATCATCGCCGCCGGCGATGGTGATAAGGTCCACGACCTCCTCAAGAAGTTCGATCCCGTGCAG
GAGGAAGCGGTCGAATAGCC

Downstream 100 bases:

>100_bases
TCCCGAAGTTCGCCGCGCCCTTCAGGGCAAGCGACCCGTCGTGGCGCTGGAAAGCACCATCATCACCCACGGTATGCCGT
ACCCCGAGAATCTCGCGACC

Product: ribokinase-like domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKIACIGGAHIDRHGLLGGPLILGTSNPGAVTSDFGGVARNVAENLSRLGREVLMVSRVGDDETGRAVVSHLNSLGIDTS
PVTISTRPTASYTAILEPNGELVLGLADMDIYDEITPELLEPCLPRLLECELWFIDANLSAATIEWISRVSAHLALAADA
VSVVKSRKLAGILDTVSPLFLNIAQAASLLGVDAFPDSEAAARAMSQHVLAGVVTAGAAGVAAWLNNEIRVLPARRAAVR
DVTGAGDALIAGTLFGITGGVPGFFRAVQLGLAAAAITVESGSTTAPHLTADLLYASQD

Sequences:

>Translated_299_residues
MKIACIGGAHIDRHGLLGGPLILGTSNPGAVTSDFGGVARNVAENLSRLGREVLMVSRVGDDETGRAVVSHLNSLGIDTS
PVTISTRPTASYTAILEPNGELVLGLADMDIYDEITPELLEPCLPRLLECELWFIDANLSAATIEWISRVSAHLALAADA
VSVVKSRKLAGILDTVSPLFLNIAQAASLLGVDAFPDSEAAARAMSQHVLAGVVTAGAAGVAAWLNNEIRVLPARRAAVR
DVTGAGDALIAGTLFGITGGVPGFFRAVQLGLAAAAITVESGSTTAPHLTADLLYASQD
>Mature_299_residues
MKIACIGGAHIDRHGLLGGPLILGTSNPGAVTSDFGGVARNVAENLSRLGREVLMVSRVGDDETGRAVVSHLNSLGIDTS
PVTISTRPTASYTAILEPNGELVLGLADMDIYDEITPELLEPCLPRLLECELWFIDANLSAATIEWISRVSAHLALAADA
VSVVKSRKLAGILDTVSPLFLNIAQAASLLGVDAFPDSEAAARAMSQHVLAGVVTAGAAGVAAWLNNEIRVLPARRAAVR
DVTGAGDALIAGTLFGITGGVPGFFRAVQLGLAAAAITVESGSTTAPHLTADLLYASQD

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=300, Percent_Identity=28.3333333333333, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788484, Length=292, Percent_Identity=27.3972602739726, Blast_Score=100, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25147470, Length=300, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 30792; Mature: 30792

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIACIGGAHIDRHGLLGGPLILGTSNPGAVTSDFGGVARNVAENLSRLGREVLMVSRVG
CEEEEECCCEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECC
DDETGRAVVSHLNSLGIDTSPVTISTRPTASYTAILEPNGELVLGLADMDIYDEITPELL
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHCCHHHH
EPCLPRLLECELWFIDANLSAATIEWISRVSAHLALAADAVSVVKSRKLAGILDTVSPLF
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIAQAASLLGVDAFPDSEAAARAMSQHVLAGVVTAGAAGVAAWLNNEIRVLPARRAAVR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEECHHHHHH
DVTGAGDALIAGTLFGITGGVPGFFRAVQLGLAAAAITVESGSTTAPHLTADLLYASQD
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHEEHEEECCC
>Mature Secondary Structure
MKIACIGGAHIDRHGLLGGPLILGTSNPGAVTSDFGGVARNVAENLSRLGREVLMVSRVG
CEEEEECCCEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECC
DDETGRAVVSHLNSLGIDTSPVTISTRPTASYTAILEPNGELVLGLADMDIYDEITPELL
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHCCHHHH
EPCLPRLLECELWFIDANLSAATIEWISRVSAHLALAADAVSVVKSRKLAGILDTVSPLF
HHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNIAQAASLLGVDAFPDSEAAARAMSQHVLAGVVTAGAAGVAAWLNNEIRVLPARRAAVR
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEECHHHHHH
DVTGAGDALIAGTLFGITGGVPGFFRAVQLGLAAAAITVESGSTTAPHLTADLLYASQD
HCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]