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The map label for this gene is gatA [H]

Identifier: 116625702

GI number: 116625702

Start: 8344734

End: 8346485

Strand: Reverse

Name: gatA [H]

Synonym: Acid_6651

Alternate gene names: 116625702

Gene position: 8346485-8344734 (Counterclockwise)

Preceding gene: 116625703

Following gene: 116625701

Centisome position: 83.75

GC content: 63.58

Gene sequence:

>1752_bases
ATGGACCGACGAACCTGGCTGCAACTGATTACAATTCTCACGGCTGCGCGCGAGGCCTACCCGCAGCAACGCGGCGGCAC
CACTCCTCCGCCGGATGCGGCAGGCCGCGGAGGACGCGGTGGACGCGGCGGGTTCCAGGATCAGCCGATGCGCGTCACCA
AGGAACAGGTCACCGGAGCGCTGAAGCTCATGGGTCTCGAGTTCCATGAAGCCGAGATCGATACCATGCTGCGCGGTGTC
AACCAGGCGGTTTACAACTACGAGTCGCTGCGCAAAGCCGATGTGCCGCTCGATACCGAGCCGGCCTTCTCCTTCCATCC
CGGATTGCCGGACCGCCAGCCGATCAAGGGGCCGCAGCGGTTTGAGACGACGATCGCCAAAACGACCATCCTGAAGGCGC
CGTCGAACCTGGAGGAGTTGGCATTCTCGCGCGTGGTGGACCTGGCGCCGCTGGTGAAATCGCGCGCGGTATCCTCCACC
GATCTCACCAAAATGTACCTGGCGCGCATGAAGAAGTACTCGCCCAAGCTGCTGTGCCTGATCACTTCGACGGAGGAGTT
CGCCCTGGAACAGGCGGCGGCCGCCGACCGGGAGATTCGCGCCGGGCACTACCGCGGGCCGCTGCACGGGATTCCGTTCG
GGCTGAAGGATCTCTTCGACACCAAGGGCATCCTGACCACTTTCGGCGCGGAACCCTACCAGACGCGCGTGGCCGATAAG
GACGCGACCGTGGTGGAGCGCCTGCATAAGGCAGGCGCGGTTCTGATGGCCAAGCTCTCCATGGGCGCGCTGGCGCAGGG
CGGGCTGTGGTTCAACGGTATGACCAAAACGCCGTGGAATTACGAGCAGACGTCCAGCGGATCGTCGGCGGGATCAGCCT
CCTCGACGGCGGCGGGACTGGTGGGCTTCACGCTGGGGACCGAGACACTGGGCTCTATCATTTCACCGAGCCAGGCCTGT
GGGACCGTGGGGCTGCGTCCCACGTACGGCCGCATCAGCCGCTTTGGAGCCATGGCGCTGAGCTGGACTATGGACAAAGT
GGGCCCGATCTGCCGCGGCGTCGAAGATTGCGCGATCGTACTCAACGCCGTGTACGGACCGGATGGCAGGGACCGCACGG
TTGGCGCGGATCCCTTCCACTGGGAGCCGCGCAAGCCGCTCGCCGGCATGCGCATCGGCATTCTGCAGAAGGATTTCGAC
CGCTACACCGGCGAACAGAAGAAAGTGTACGACCAGGCGCTGGAAGATCTCAAGAAGGCCGGGGTGAAGATGACGCCGAC
GGAATTCGTGGAAGACGGTCCCACGATCCGCTTCCTGTTGAGCGCGGAGGCCGCGGCGGCCTTTGACGATATCACGCGCG
ATGGGCAGGTTCGCAACTTGCGCGGGCAATCGCCCGGCGACTGGCCCAATCAGTTCCGCACGTCGCGCATGATTCCGGCA
GTGGAATACATTCGTGCGCAGCGTGCGCGGACCCAATTGATTCGCAAATTCGAAAAGTTCATGGACGATTGGGATGTGCT
CGTGATGCCGCCCAACTCGCTGTTGACCACCACCAACTTGACCGGCAATCCTCAGGTGGTGATGAAGTGCGGGTTCACGG
AAGGCGCGAAGAGCGATGGCACGAAGGTGCAGGTGCCGCGCTCCATCAGTTTCCTGGGAAAGATTTACGATGAGGGCGCG
CCGCTGCGCGTGGCGCTGGCCTACGAGCAGGCCACCGAGTGGCACAAGCAGAATCCGGTGCTGGAGGTATGA

Upstream 100 bases:

>100_bases
CCGCGCATCAGAGAGATGCTGGGCGCGTAGCCCGGCTCGCTCGACGTCCTTCTTTCTCGAGCGTGTATCCCTTCTTGAGC
GTGTATCATAGAGAAAATTT

Downstream 100 bases:

>100_bases
CGATGAACCGTCGGGTATTTCTAGCCACAGCCGCGGCGGGCGTCGCCGCGGCGCAGCAGAGCGATACTCCAGAGCCGATC
CGCAAGCTCCGCCCCATGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MDRRTWLQLITILTAAREAYPQQRGGTTPPPDAAGRGGRGGRGGFQDQPMRVTKEQVTGALKLMGLEFHEAEIDTMLRGV
NQAVYNYESLRKADVPLDTEPAFSFHPGLPDRQPIKGPQRFETTIAKTTILKAPSNLEELAFSRVVDLAPLVKSRAVSST
DLTKMYLARMKKYSPKLLCLITSTEEFALEQAAAADREIRAGHYRGPLHGIPFGLKDLFDTKGILTTFGAEPYQTRVADK
DATVVERLHKAGAVLMAKLSMGALAQGGLWFNGMTKTPWNYEQTSSGSSAGSASSTAAGLVGFTLGTETLGSIISPSQAC
GTVGLRPTYGRISRFGAMALSWTMDKVGPICRGVEDCAIVLNAVYGPDGRDRTVGADPFHWEPRKPLAGMRIGILQKDFD
RYTGEQKKVYDQALEDLKKAGVKMTPTEFVEDGPTIRFLLSAEAAAAFDDITRDGQVRNLRGQSPGDWPNQFRTSRMIPA
VEYIRAQRARTQLIRKFEKFMDDWDVLVMPPNSLLTTTNLTGNPQVVMKCGFTEGAKSDGTKVQVPRSISFLGKIYDEGA
PLRVALAYEQATEWHKQNPVLEV

Sequences:

>Translated_583_residues
MDRRTWLQLITILTAAREAYPQQRGGTTPPPDAAGRGGRGGRGGFQDQPMRVTKEQVTGALKLMGLEFHEAEIDTMLRGV
NQAVYNYESLRKADVPLDTEPAFSFHPGLPDRQPIKGPQRFETTIAKTTILKAPSNLEELAFSRVVDLAPLVKSRAVSST
DLTKMYLARMKKYSPKLLCLITSTEEFALEQAAAADREIRAGHYRGPLHGIPFGLKDLFDTKGILTTFGAEPYQTRVADK
DATVVERLHKAGAVLMAKLSMGALAQGGLWFNGMTKTPWNYEQTSSGSSAGSASSTAAGLVGFTLGTETLGSIISPSQAC
GTVGLRPTYGRISRFGAMALSWTMDKVGPICRGVEDCAIVLNAVYGPDGRDRTVGADPFHWEPRKPLAGMRIGILQKDFD
RYTGEQKKVYDQALEDLKKAGVKMTPTEFVEDGPTIRFLLSAEAAAAFDDITRDGQVRNLRGQSPGDWPNQFRTSRMIPA
VEYIRAQRARTQLIRKFEKFMDDWDVLVMPPNSLLTTTNLTGNPQVVMKCGFTEGAKSDGTKVQVPRSISFLGKIYDEGA
PLRVALAYEQATEWHKQNPVLEV
>Mature_583_residues
MDRRTWLQLITILTAAREAYPQQRGGTTPPPDAAGRGGRGGRGGFQDQPMRVTKEQVTGALKLMGLEFHEAEIDTMLRGV
NQAVYNYESLRKADVPLDTEPAFSFHPGLPDRQPIKGPQRFETTIAKTTILKAPSNLEELAFSRVVDLAPLVKSRAVSST
DLTKMYLARMKKYSPKLLCLITSTEEFALEQAAAADREIRAGHYRGPLHGIPFGLKDLFDTKGILTTFGAEPYQTRVADK
DATVVERLHKAGAVLMAKLSMGALAQGGLWFNGMTKTPWNYEQTSSGSSAGSASSTAAGLVGFTLGTETLGSIISPSQAC
GTVGLRPTYGRISRFGAMALSWTMDKVGPICRGVEDCAIVLNAVYGPDGRDRTVGADPFHWEPRKPLAGMRIGILQKDFD
RYTGEQKKVYDQALEDLKKAGVKMTPTEFVEDGPTIRFLLSAEAAAAFDDITRDGQVRNLRGQSPGDWPNQFRTSRMIPA
VEYIRAQRARTQLIRKFEKFMDDWDVLVMPPNSLLTTTNLTGNPQVVMKCGFTEGAKSDGTKVQVPRSISFLGKIYDEGA
PLRVALAYEQATEWHKQNPVLEV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=257, Percent_Identity=33.0739299610895, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=26.3157894736842, Blast_Score=90, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=25.6302521008403, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=338, Percent_Identity=26.0355029585799, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=285, Percent_Identity=27.719298245614, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=219, Percent_Identity=28.310502283105, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=25.5696202531646, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=357, Percent_Identity=28.2913165266106, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648113, Length=228, Percent_Identity=38.1578947368421, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644968, Length=262, Percent_Identity=28.2442748091603, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356731, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=27.6422764227642, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=27.6422764227642, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652985, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922090, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24652981, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24652983, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161078093, Length=246, Percent_Identity=28.0487804878049, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63988; Mature: 63988

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEECCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure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CEEECCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA