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The map label for this gene is waaA [H]

Identifier: 116625550

GI number: 116625550

Start: 8142502

End: 8144835

Strand: Direct

Name: waaA [H]

Synonym: Acid_6497

Alternate gene names: 116625550

Gene position: 8142502-8144835 (Clockwise)

Preceding gene: 116625545

Following gene: 116625551

Centisome position: 81.71

GC content: 63.84

Gene sequence:

>2334_bases
TTGAAAACAAAAGGCATTTATTTCCTCTACCGAGTCCTTCAGGCGTTTGTGTTGCCCGCCCTCTTATTGTATTTCCTCGT
GCGCAGCTGCGGCGACCGGAAATACTGGCGCAGTCTCCCGCAGCGGTTCGGATTTCTTCCGCATTCGTTTAGACAGATTG
GCCCGGGCGCGATCTGGTTGCATGCAGTTTCCATGGGAGAGGTCCTGGCCTGTGTGGAATTCGCACGGCGCCTGAAGACA
GAGTTCCCCAGATCGAGCCTCTTCCTGTCCACCGCTACACTGGCAGGACATGCCACGGCGGAGCAGAAACTCACCTCGAT
CGCCGACGGCATCTTTTTCGCGCCGGTGGATTATGTGTGGGTCGTGCGCCGAGTTCTGCGGACGCTGAAGCCCGCGCTCG
TCATCATCGCGGAAACGGAGATCTGGCCCAATCTCTTGCGTGAGGTCCATCGCACCGGTGCGGGGCTGGCAATCGTCAAC
GCGCGGATCTCCGACAAGGCACTGCCGAAATATTTGCGCTTGCGCTGGATCTTTCCGGTGGTCCTCGCCGCGGTAGACCG
GGTCCTGGCGCAGACCGAAGAGATCGCCGAACGCTTTCGCATGCTCGGCGCCGAGCGCGTCCTGGTGGGCGGCAACCTCA
AGTTCGATTTCGAAGCGCGTGCGGCAGGCACGGATTCGCCGGTGGTTCAAATGCTGCAGCGGGTGAACCCCAGGAAGGTG
TGGATTGCCGCCAGCACCATGGCTGACGACGTCATCGACGAGGACGACGCCGTGATCGGCGCCTGGCAATCCCTGGCGCG
GCGCGATGTCTTCCTGATCCTGGTGCCGCGCAAGCCGAAGCGCTTCGAGGTTGTGTCCGAAAAGTTGCAAGCCGCCGGGA
TCCGGTATGCCCGGCGTTCCCGGTTGGACGCCGACGGGCCCAAACCCGACGTACTGCTGCTCGATTCCATCGGCGAACTG
GGATCGCTGTTCGCCTATGCGGACGTGGTGTTCATGGGTGGAACCCTAACGGCGCGCGGTGGTCACAACATCCTGGAACC
CGCGCTCTTCGGCAAACCCGTGATCGTGGGTCCGCATATGGAAAATTTCCAGGCGATCGCGGATGAGTTTCGCGCCGCGC
GGGCGATGGCGGAGATCGGGACCGCGGACGAGTTGGCCGGCGCAGTGGACCGCGCACTCGCCGCCGGCGGCGAAATGGGA
CAGCGCGCGCGTATCTGCGCCGAGGCGCGCCGCGGGGCCACGGCGTTCGCGATTGAGCAGATGCGCGAGATCCAGCGGAT
ACCGCGCTACCGTCCGCCGATGCCGTGGTTCCTTGTGGCTCGGGCACTCTCGGAAGCGTGGAAATGGGAGGCCAAACGGC
GCCAGGTCCGCGATTACGCGCACCGAAAGCGCCTCAAGTCGCCAGTGATCAGTATCGGCAACCTCACGATGGGCGGCACC
GGAAAAACGCCCTGCGTCTTGCGGCTCACGGAACTGCTGCGCGAGCGTGGCCATAGGCCCGGCATTCTGACACGCGGCTA
CGGACGCACTTCGCCGGTGGATCACATGGCGCTGGCGGCGGGCGCCAACGTCCGCCCCGAAGAATCGGGCGACGAGCCGC
AGATCTTCCTGCGTTCGCGGGTGGCGCCCGTGGGAATCGGGGCCGACCGTTTTCAAACGGGGTCTCTGCTCGCCGAGCGC
TTCGGCACCGATGTGGTTGTGTTGGACGATGGCTTTCAACATGTGAAGCTGGCGCGCAATTTCGACCTGGTGCTGATCGA
TGCGCTGAAACCTTTCGGCGGAGGCGAAATCTTCCCGGCAGGCCGCCTGCGCGAGCCGGTGCAGGGAATAGCCCGCGCGG
ATGCGATTATTATCACGCGCAGCGACGCGTCCGACCTGAGGCCGGCGATCGAAACCGTGGTGCGCCGGTGGAATGCCCGC
ATCCCCATCTTTCGGGCGCGGATTCAACCCGAGTGGTGGGTGGAGCACCGGACCGGCAAGCGGCATGAGGCAGACAAGTT
CCACATCGAACGTCCCGGCGTGTTCTGCGGACTGGGGAATCCCGTGGGCTTCTACCGGACACTCGAGAGCCTCGGACTGC
GCCACATCGATTGCGTGGAGTTCGAAGACCACCACCGGTATCGCTCCAAGGAACTGGAACGGATTGCCGGCCAGTTCCGG
CGAAGAGGCGCGGCAACCGTGGTCACCACCGAGAAGGACGCCATCAACCTCTGCGAGGGCGCCGACGACATGCTCGCACC
GCTCCCCCTCTACTGGCTCAAGGTCGGCATGCGGATCGAGGGAGAAGAGGAGTTGCTGGCCCTCATCGAGAAGAGCATGG
CTCCTCGCGCCTAA

Upstream 100 bases:

>100_bases
TTGGTGGCTCGCGTAGCTGGCGGCGGGCGCGGCCTTGAACGGCGTCTCCTTGTCGGCAGCTACGCACGCGGATATACTCA
AGAAAAGAAGAGCCAAACGT

Downstream 100 bases:

>100_bases
GAAGTCTCTCGGGGCGCCGACCTACATGCTATCGCTTACTTATCCGTCTTACTCGAATTGAATAAACTCCAATGCCAATA
CGACGGGTGTTATCTAAGTT

Product: lipid-A-disaccharide synthase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MKTKGIYFLYRVLQAFVLPALLLYFLVRSCGDRKYWRSLPQRFGFLPHSFRQIGPGAIWLHAVSMGEVLACVEFARRLKT
EFPRSSLFLSTATLAGHATAEQKLTSIADGIFFAPVDYVWVVRRVLRTLKPALVIIAETEIWPNLLREVHRTGAGLAIVN
ARISDKALPKYLRLRWIFPVVLAAVDRVLAQTEEIAERFRMLGAERVLVGGNLKFDFEARAAGTDSPVVQMLQRVNPRKV
WIAASTMADDVIDEDDAVIGAWQSLARRDVFLILVPRKPKRFEVVSEKLQAAGIRYARRSRLDADGPKPDVLLLDSIGEL
GSLFAYADVVFMGGTLTARGGHNILEPALFGKPVIVGPHMENFQAIADEFRAARAMAEIGTADELAGAVDRALAAGGEMG
QRARICAEARRGATAFAIEQMREIQRIPRYRPPMPWFLVARALSEAWKWEAKRRQVRDYAHRKRLKSPVISIGNLTMGGT
GKTPCVLRLTELLRERGHRPGILTRGYGRTSPVDHMALAAGANVRPEESGDEPQIFLRSRVAPVGIGADRFQTGSLLAER
FGTDVVVLDDGFQHVKLARNFDLVLIDALKPFGGGEIFPAGRLREPVQGIARADAIIITRSDASDLRPAIETVVRRWNAR
IPIFRARIQPEWWVEHRTGKRHEADKFHIERPGVFCGLGNPVGFYRTLESLGLRHIDCVEFEDHHRYRSKELERIAGQFR
RRGAATVVTTEKDAINLCEGADDMLAPLPLYWLKVGMRIEGEEELLALIEKSMAPRA

Sequences:

>Translated_777_residues
MKTKGIYFLYRVLQAFVLPALLLYFLVRSCGDRKYWRSLPQRFGFLPHSFRQIGPGAIWLHAVSMGEVLACVEFARRLKT
EFPRSSLFLSTATLAGHATAEQKLTSIADGIFFAPVDYVWVVRRVLRTLKPALVIIAETEIWPNLLREVHRTGAGLAIVN
ARISDKALPKYLRLRWIFPVVLAAVDRVLAQTEEIAERFRMLGAERVLVGGNLKFDFEARAAGTDSPVVQMLQRVNPRKV
WIAASTMADDVIDEDDAVIGAWQSLARRDVFLILVPRKPKRFEVVSEKLQAAGIRYARRSRLDADGPKPDVLLLDSIGEL
GSLFAYADVVFMGGTLTARGGHNILEPALFGKPVIVGPHMENFQAIADEFRAARAMAEIGTADELAGAVDRALAAGGEMG
QRARICAEARRGATAFAIEQMREIQRIPRYRPPMPWFLVARALSEAWKWEAKRRQVRDYAHRKRLKSPVISIGNLTMGGT
GKTPCVLRLTELLRERGHRPGILTRGYGRTSPVDHMALAAGANVRPEESGDEPQIFLRSRVAPVGIGADRFQTGSLLAER
FGTDVVVLDDGFQHVKLARNFDLVLIDALKPFGGGEIFPAGRLREPVQGIARADAIIITRSDASDLRPAIETVVRRWNAR
IPIFRARIQPEWWVEHRTGKRHEADKFHIERPGVFCGLGNPVGFYRTLESLGLRHIDCVEFEDHHRYRSKELERIAGQFR
RRGAATVVTTEKDAINLCEGADDMLAPLPLYWLKVGMRIEGEEELLALIEKSMAPRA
>Mature_777_residues
MKTKGIYFLYRVLQAFVLPALLLYFLVRSCGDRKYWRSLPQRFGFLPHSFRQIGPGAIWLHAVSMGEVLACVEFARRLKT
EFPRSSLFLSTATLAGHATAEQKLTSIADGIFFAPVDYVWVVRRVLRTLKPALVIIAETEIWPNLLREVHRTGAGLAIVN
ARISDKALPKYLRLRWIFPVVLAAVDRVLAQTEEIAERFRMLGAERVLVGGNLKFDFEARAAGTDSPVVQMLQRVNPRKV
WIAASTMADDVIDEDDAVIGAWQSLARRDVFLILVPRKPKRFEVVSEKLQAAGIRYARRSRLDADGPKPDVLLLDSIGEL
GSLFAYADVVFMGGTLTARGGHNILEPALFGKPVIVGPHMENFQAIADEFRAARAMAEIGTADELAGAVDRALAAGGEMG
QRARICAEARRGATAFAIEQMREIQRIPRYRPPMPWFLVARALSEAWKWEAKRRQVRDYAHRKRLKSPVISIGNLTMGGT
GKTPCVLRLTELLRERGHRPGILTRGYGRTSPVDHMALAAGANVRPEESGDEPQIFLRSRVAPVGIGADRFQTGSLLAER
FGTDVVVLDDGFQHVKLARNFDLVLIDALKPFGGGEIFPAGRLREPVQGIARADAIIITRSDASDLRPAIETVVRRWNAR
IPIFRARIQPEWWVEHRTGKRHEADKFHIERPGVFCGLGNPVGFYRTLESLGLRHIDCVEFEDHHRYRSKELERIAGQFR
RRGAATVVTTEKDAINLCEGADDMLAPLPLYWLKVGMRIEGEEELLALIEKSMAPRA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=391, Percent_Identity=33.7595907928389, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1787144, Length=346, Percent_Identity=30.9248554913295, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 86966; Mature: 86966

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKGIYFLYRVLQAFVLPALLLYFLVRSCGDRKYWRSLPQRFGFLPHSFRQIGPGAIWL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEE
HAVSMGEVLACVEFARRLKTEFPRSSLFLSTATLAGHATAEQKLTSIADGIFFAPVDYVW
EHHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHHCCHHHHHHHHHHHCCHHCCHHHHHH
VVRRVLRTLKPALVIIAETEIWPNLLREVHRTGAGLAIVNARISDKALPKYLRLRWIFPV
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
VLAAVDRVLAQTEEIAERFRMLGAERVLVGGNLKFDFEARAAGTDSPVVQMLQRVNPRKV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEE
WIAASTMADDVIDEDDAVIGAWQSLARRDVFLILVPRKPKRFEVVSEKLQAAGIRYARRS
EEEEHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RLDADGPKPDVLLLDSIGELGSLFAYADVVFMGGTLTARGGHNILEPALFGKPVIVGPHM
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCEEECCCH
ENFQAIADEFRAARAMAEIGTADELAGAVDRALAAGGEMGQRARICAEARRGATAFAIEQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
MREIQRIPRYRPPMPWFLVARALSEAWKWEAKRRQVRDYAHRKRLKSPVISIGNLTMGGT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCC
GKTPCVLRLTELLRERGHRPGILTRGYGRTSPVDHMALAAGANVRPEESGDEPQIFLRSR
CCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHC
VAPVGIGADRFQTGSLLAERFGTDVVVLDDGFQHVKLARNFDLVLIDALKPFGGGEIFPA
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCC
GRLREPVQGIARADAIIITRSDASDLRPAIETVVRRWNARIPIFRARIQPEWWVEHRTGK
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCC
RHEADKFHIERPGVFCGLGNPVGFYRTLESLGLRHIDCVEFEDHHRYRSKELERIAGQFR
CCCCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RRGAATVVTTEKDAINLCEGADDMLAPLPLYWLKVGMRIEGEEELLALIEKSMAPRA
HCCCCEEEECCCHHHHHHCCCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTKGIYFLYRVLQAFVLPALLLYFLVRSCGDRKYWRSLPQRFGFLPHSFRQIGPGAIWL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEE
HAVSMGEVLACVEFARRLKTEFPRSSLFLSTATLAGHATAEQKLTSIADGIFFAPVDYVW
EHHHHHHHHHHHHHHHHHHHHCCCHHHEEEHHHHHCCHHHHHHHHHHHCCHHCCHHHHHH
VVRRVLRTLKPALVIIAETEIWPNLLREVHRTGAGLAIVNARISDKALPKYLRLRWIFPV
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHH
VLAAVDRVLAQTEEIAERFRMLGAERVLVGGNLKFDFEARAAGTDSPVVQMLQRVNPRKV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCHHHHHHHCCCCCEE
WIAASTMADDVIDEDDAVIGAWQSLARRDVFLILVPRKPKRFEVVSEKLQAAGIRYARRS
EEEEHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
RLDADGPKPDVLLLDSIGELGSLFAYADVVFMGGTLTARGGHNILEPALFGKPVIVGPHM
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHCCCCEEECCCH
ENFQAIADEFRAARAMAEIGTADELAGAVDRALAAGGEMGQRARICAEARRGATAFAIEQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
MREIQRIPRYRPPMPWFLVARALSEAWKWEAKRRQVRDYAHRKRLKSPVISIGNLTMGGT
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCC
GKTPCVLRLTELLRERGHRPGILTRGYGRTSPVDHMALAAGANVRPEESGDEPQIFLRSR
CCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHC
VAPVGIGADRFQTGSLLAERFGTDVVVLDDGFQHVKLARNFDLVLIDALKPFGGGEIFPA
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCCCC
GRLREPVQGIARADAIIITRSDASDLRPAIETVVRRWNARIPIFRARIQPEWWVEHRTGK
HHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCC
RHEADKFHIERPGVFCGLGNPVGFYRTLESLGLRHIDCVEFEDHHRYRSKELERIAGQFR
CCCCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
RRGAATVVTTEKDAINLCEGADDMLAPLPLYWLKVGMRIEGEEELLALIEKSMAPRA
HCCCCEEEECCCHHHHHHCCCCHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]