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The map label for this gene is zraS [H]

Identifier: 116625412

GI number: 116625412

Start: 7976034

End: 7978436

Strand: Reverse

Name: zraS [H]

Synonym: Acid_6357

Alternate gene names: 116625412

Gene position: 7978436-7976034 (Counterclockwise)

Preceding gene: 116625413

Following gene: 116625411

Centisome position: 80.06

GC content: 64.29

Gene sequence:

>2403_bases
ATGGCTGCAAACGCTGGTGCGATCCGCCTAGCTGCGCTGCGCGTAACGGCACTGGGAGTGTCTCTGGCCGTGCTTTGCCT
GCCCGCCGCGGCCCAGATCCGAATCACCTTATCGCAACTCAGCTTCCGCTCCGGACCAACATATTCAGCGGCTTACGCTG
GCCAGGAAGTGATGATTCGCGGAGTGGTATCGGCGCCGGCGATTCATTTCCTGGAATACACCATGCTGGCCCTTCAGGAT
GGCCGTAATGGCGGCGTGCTGAAGGTCCCGGCGGGCGACACCTCCCTGGAAAGGTTCCGCCCAGGCGATGAATTGGAAGT
GCGGGGCAAAGTCGCGCTGCAGTTCGGGATGACGGTGGTGCTGCCCGAAAGCATCGCCCCCGGCGAGCACAAGGCACCCC
CTCAGCCAAAGGAAATGTCCCCCGAGGAGGCGCAGAGCCTGGCTCACCTGGGGGAACTGGTGCGCGTTCGGAGTACGGTG
GTCACCCAGTACCGCAACTCGGGCGGTACCGGGATCTTCGTGGCGGCTGCCAAAGATAACTACCGCATTTTTATCCCGCG
GCCGCCCGGCAAAGACTATGTCAGCCTGAATTCGATCCGTCCCGGCGAAACCGTGCAGGTCACCGGCGTTGCTTTGCAGT
ATTGTCCGGCGCCGCCGTTCAACACCGGCTTCCAACTCCTGATCGGCAGCGTCAATGACATCGTCCCGGTGGATTTGCCC
GCTACGCTTCCCCAGCCTCTGATCGCGAGCGGCATTACGGTAGTTCTGCTGGTCGGCTTTTTCCTGTGGACGCGGGAAAG
GCGCTTGCGGTCGCAGCGCCGCCGGCTGCGCAGGACCTATAAGCTGGGCGAAGAAATTTTGAGCGCGTCCTCAGCGGCCA
CCATCCTCAAGCGAATTTCCGAGGCGCTGCCGAGCATCATCGGCGTGACCCGCGTGCGTTTGTACGTCTACAACCGGACG
GCAAAGACGCTGGACGCGATCGAAGACGAGCATGGCGCATCGACCTCGATTTCGCTATCCTCGCCGCCGGGCGGGACGCC
GGCCGGCGCGGTCGCATGCTTCCATTACCGCACGCTGCTGGTGATTCCCGATATCGACCGCAGCCCCTTCCCGATCGCCG
GACAAAAGGGCGAGCGCAGCCCGAAGAGCCTGCTGTTCGTGCCGATGATGGCGCAGAGCGAGGTGATCGGGGTCCTGGAA
TTGGATCAGGACGACCGGGTCCGCGATTTTACGGCCGACGAGCAGGCGCTGGCTCAGCACCTCGGGAATCAGATCGGCGT
GGCCATCCGGCTGCTGGATCAGCGTACGGTACAGGAGCAGTTGTTCCGTACCGAAAAGATGGCAGCGGTGGGGCGGTTGA
TCTCAGGCGTGGTGAACGAGTTGCAGACGCCGCTGGCTTCCATCACGGACCTGGCGACGCGGGCGGTGGAAAAGGCGCGC
GGCGGACCGGCCGAGCGCGAGGTTTCGGCGATCGCGACCGAAGCCGCCAAAGCCAGCGGCATGGTTACGCGGCTGGTGTC
CTTCGCGGCGGCCGAGCAGGTGGAAGCGCGCCCGGTGGATGTCACGGGCCTGCTGCGCAACCTGATCGAGTTTCGCGAAG
GCGATTGGAAGGCATCCGGAATCCGCGTGCGCGACCTGACCGCGCACGAACCGATGTTCGTTCACGGCTCGCAGGGGCAG
ATGGAACAGGTCTTCCTCAACCTGCTGGTACATGCGGAACAGTCGCTGGCCGACGCGCCTCAGAAATCGATTACGGTGCG
CACCAGCCTGCTGGCGAAGCGCCTCCTGGTGGAGATCTCTTTCACCTCGCCGGCGGATTTGCGCAAGCCGGAGGATACGG
CGGCTGTACTTGGCGTGACGCGCAGCGTAATCGCGGGGCATGGCGGGGAGGTCCGGCTGATCGACAAGAACAACGCCGAC
CCGCGGTTTGAAGTGGAGCTGCCTGCCTTGGTGAAAGATCGTGCGATGCCCGCGACGGGCGCGACCGTGGCGGTGCGCGA
TTCCTCCCGGCGCATGACGGCCCTGGTGATCGAGCCGGACGAAGCCATGCAGCGGCAGATTCTGGCGCTGCTCTCCGGCC
ACGGCTATCGAGTGGTTCCGGTGTCCAATTCCGATACGGGGCTCGACCTGGCGCATCGTATGCGGTTCGATGCGGCGTTT
TGCTCCGTGCACGCACCGGGGCTGAACTGGGTGGAACTCTCCGAACGAATGCACGCGCGAGTGGGCGGCTTCATCCTGCT
CTCCGACGGCTACGACGCGGAACTGGCCGCCGATTTCGAAGGGGACGGCCGCTTCGTACTCGCCAAGCCGGTGCAAGAGA
AGGAGCTGGAGCGCGTCCTGCAGGCACTCGAACCACCCGCCCCGAACGCCAACGTAATTCCCTTCAAGACCGGCACCGCG
TAG

Upstream 100 bases:

>100_bases
TGGCTGCCGGGATGAGGGTCATCGGTCTTCGTACTACTTTCGTTAACTTGCCCGGAACGGTGCTAACCGTCGATAATTTT
TTGAGCGGTAACTTGGAGAC

Downstream 100 bases:

>100_bases
CTGTCTCGTCGCTCCGCACGAAAATCCACCCCCAACGGGCGCAACAATGACCGCCAACCATCACCGCCAACAATGAGCGC
CAACAATGAGCGCGCAACAA

Product: response regulator receiver modulated GAF sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MAANAGAIRLAALRVTALGVSLAVLCLPAAAQIRITLSQLSFRSGPTYSAAYAGQEVMIRGVVSAPAIHFLEYTMLALQD
GRNGGVLKVPAGDTSLERFRPGDELEVRGKVALQFGMTVVLPESIAPGEHKAPPQPKEMSPEEAQSLAHLGELVRVRSTV
VTQYRNSGGTGIFVAAAKDNYRIFIPRPPGKDYVSLNSIRPGETVQVTGVALQYCPAPPFNTGFQLLIGSVNDIVPVDLP
ATLPQPLIASGITVVLLVGFFLWTRERRLRSQRRRLRRTYKLGEEILSASSAATILKRISEALPSIIGVTRVRLYVYNRT
AKTLDAIEDEHGASTSISLSSPPGGTPAGAVACFHYRTLLVIPDIDRSPFPIAGQKGERSPKSLLFVPMMAQSEVIGVLE
LDQDDRVRDFTADEQALAQHLGNQIGVAIRLLDQRTVQEQLFRTEKMAAVGRLISGVVNELQTPLASITDLATRAVEKAR
GGPAEREVSAIATEAAKASGMVTRLVSFAAAEQVEARPVDVTGLLRNLIEFREGDWKASGIRVRDLTAHEPMFVHGSQGQ
MEQVFLNLLVHAEQSLADAPQKSITVRTSLLAKRLLVEISFTSPADLRKPEDTAAVLGVTRSVIAGHGGEVRLIDKNNAD
PRFEVELPALVKDRAMPATGATVAVRDSSRRMTALVIEPDEAMQRQILALLSGHGYRVVPVSNSDTGLDLAHRMRFDAAF
CSVHAPGLNWVELSERMHARVGGFILLSDGYDAELAADFEGDGRFVLAKPVQEKELERVLQALEPPAPNANVIPFKTGTA

Sequences:

>Translated_800_residues
MAANAGAIRLAALRVTALGVSLAVLCLPAAAQIRITLSQLSFRSGPTYSAAYAGQEVMIRGVVSAPAIHFLEYTMLALQD
GRNGGVLKVPAGDTSLERFRPGDELEVRGKVALQFGMTVVLPESIAPGEHKAPPQPKEMSPEEAQSLAHLGELVRVRSTV
VTQYRNSGGTGIFVAAAKDNYRIFIPRPPGKDYVSLNSIRPGETVQVTGVALQYCPAPPFNTGFQLLIGSVNDIVPVDLP
ATLPQPLIASGITVVLLVGFFLWTRERRLRSQRRRLRRTYKLGEEILSASSAATILKRISEALPSIIGVTRVRLYVYNRT
AKTLDAIEDEHGASTSISLSSPPGGTPAGAVACFHYRTLLVIPDIDRSPFPIAGQKGERSPKSLLFVPMMAQSEVIGVLE
LDQDDRVRDFTADEQALAQHLGNQIGVAIRLLDQRTVQEQLFRTEKMAAVGRLISGVVNELQTPLASITDLATRAVEKAR
GGPAEREVSAIATEAAKASGMVTRLVSFAAAEQVEARPVDVTGLLRNLIEFREGDWKASGIRVRDLTAHEPMFVHGSQGQ
MEQVFLNLLVHAEQSLADAPQKSITVRTSLLAKRLLVEISFTSPADLRKPEDTAAVLGVTRSVIAGHGGEVRLIDKNNAD
PRFEVELPALVKDRAMPATGATVAVRDSSRRMTALVIEPDEAMQRQILALLSGHGYRVVPVSNSDTGLDLAHRMRFDAAF
CSVHAPGLNWVELSERMHARVGGFILLSDGYDAELAADFEGDGRFVLAKPVQEKELERVLQALEPPAPNANVIPFKTGTA
>Mature_799_residues
AANAGAIRLAALRVTALGVSLAVLCLPAAAQIRITLSQLSFRSGPTYSAAYAGQEVMIRGVVSAPAIHFLEYTMLALQDG
RNGGVLKVPAGDTSLERFRPGDELEVRGKVALQFGMTVVLPESIAPGEHKAPPQPKEMSPEEAQSLAHLGELVRVRSTVV
TQYRNSGGTGIFVAAAKDNYRIFIPRPPGKDYVSLNSIRPGETVQVTGVALQYCPAPPFNTGFQLLIGSVNDIVPVDLPA
TLPQPLIASGITVVLLVGFFLWTRERRLRSQRRRLRRTYKLGEEILSASSAATILKRISEALPSIIGVTRVRLYVYNRTA
KTLDAIEDEHGASTSISLSSPPGGTPAGAVACFHYRTLLVIPDIDRSPFPIAGQKGERSPKSLLFVPMMAQSEVIGVLEL
DQDDRVRDFTADEQALAQHLGNQIGVAIRLLDQRTVQEQLFRTEKMAAVGRLISGVVNELQTPLASITDLATRAVEKARG
GPAEREVSAIATEAAKASGMVTRLVSFAAAEQVEARPVDVTGLLRNLIEFREGDWKASGIRVRDLTAHEPMFVHGSQGQM
EQVFLNLLVHAEQSLADAPQKSITVRTSLLAKRLLVEISFTSPADLRKPEDTAAVLGVTRSVIAGHGGEVRLIDKNNADP
RFEVELPALVKDRAMPATGATVAVRDSSRRMTALVIEPDEAMQRQILALLSGHGYRVVPVSNSDTGLDLAHRMRFDAAFC
SVHAPGLNWVELSERMHARVGGFILLSDGYDAELAADFEGDGRFVLAKPVQEKELERVLQALEPPAPNANVIPFKTGTA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=25.5411255411255, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 86393; Mature: 86262

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANAGAIRLAALRVTALGVSLAVLCLPAAAQIRITLSQLSFRSGPTYSAAYAGQEVMIR
CCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHCCCHHEEE
GVVSAPAIHFLEYTMLALQDGRNGGVLKVPAGDTSLERFRPGDELEVRGKVALQFGMTVV
EHHHCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCCCEEECEEEEEEECCEEE
LPESIAPGEHKAPPQPKEMSPEEAQSLAHLGELVRVRSTVVTQYRNSGGTGIFVAAAKDN
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
YRIFIPRPPGKDYVSLNSIRPGETVQVTGVALQYCPAPPFNTGFQLLIGSVNDIVPVDLP
EEEEEECCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCHHEEECCCCCEEECCCC
ATLPQPLIASGITVVLLVGFFLWTRERRLRSQRRRLRRTYKLGEEILSASSAATILKRIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALPSIIGVTRVRLYVYNRTAKTLDAIEDEHGASTSISLSSPPGGTPAGAVACFHYRTLL
HHHHHHHHHHEEEEEEEECCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHEEEEEEE
VIPDIDRSPFPIAGQKGERSPKSLLFVPMMAQSEVIGVLELDQDDRVRDFTADEQALAQH
EEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LGNQIGVAIRLLDQRTVQEQLFRTEKMAAVGRLISGVVNELQTPLASITDLATRAVEKAR
CCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGPAEREVSAIATEAAKASGMVTRLVSFAAAEQVEARPVDVTGLLRNLIEFREGDWKASG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
IRVRDLTAHEPMFVHGSQGQMEQVFLNLLVHAEQSLADAPQKSITVRTSLLAKRLLVEIS
EEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHEEEEE
FTSPADLRKPEDTAAVLGVTRSVIAGHGGEVRLIDKNNADPRFEVELPALVKDRAMPATG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHCCCCCCC
ATVAVRDSSRRMTALVIEPDEAMQRQILALLSGHGYRVVPVSNSDTGLDLAHRMRFDAAF
CEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
CSVHAPGLNWVELSERMHARVGGFILLSDGYDAELAADFEGDGRFVLAKPVQEKELERVL
EEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHH
QALEPPAPNANVIPFKTGTA
HHHCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
AANAGAIRLAALRVTALGVSLAVLCLPAAAQIRITLSQLSFRSGPTYSAAYAGQEVMIR
CCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHCCCHHEEE
GVVSAPAIHFLEYTMLALQDGRNGGVLKVPAGDTSLERFRPGDELEVRGKVALQFGMTVV
EHHHCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHCCCCCCEEECEEEEEEECCEEE
LPESIAPGEHKAPPQPKEMSPEEAQSLAHLGELVRVRSTVVTQYRNSGGTGIFVAAAKDN
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
YRIFIPRPPGKDYVSLNSIRPGETVQVTGVALQYCPAPPFNTGFQLLIGSVNDIVPVDLP
EEEEEECCCCCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCHHEEECCCCCEEECCCC
ATLPQPLIASGITVVLLVGFFLWTRERRLRSQRRRLRRTYKLGEEILSASSAATILKRIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALPSIIGVTRVRLYVYNRTAKTLDAIEDEHGASTSISLSSPPGGTPAGAVACFHYRTLL
HHHHHHHHHHEEEEEEEECCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHEEEEEEE
VIPDIDRSPFPIAGQKGERSPKSLLFVPMMAQSEVIGVLELDQDDRVRDFTADEQALAQH
EEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHH
LGNQIGVAIRLLDQRTVQEQLFRTEKMAAVGRLISGVVNELQTPLASITDLATRAVEKAR
CCCHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGPAEREVSAIATEAAKASGMVTRLVSFAAAEQVEARPVDVTGLLRNLIEFREGDWKASG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCC
IRVRDLTAHEPMFVHGSQGQMEQVFLNLLVHAEQSLADAPQKSITVRTSLLAKRLLVEIS
EEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHEEEEE
FTSPADLRKPEDTAAVLGVTRSVIAGHGGEVRLIDKNNADPRFEVELPALVKDRAMPATG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHCCCCCCC
ATVAVRDSSRRMTALVIEPDEAMQRQILALLSGHGYRVVPVSNSDTGLDLAHRMRFDAAF
CEEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHH
CSVHAPGLNWVELSERMHARVGGFILLSDGYDAELAADFEGDGRFVLAKPVQEKELERVL
EEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHH
QALEPPAPNANVIPFKTGTA
HHHCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]