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The map label for this gene is queA

Identifier: 116625409

GI number: 116625409

Start: 7972900

End: 7973958

Strand: Direct

Name: queA

Synonym: Acid_6354

Alternate gene names: 116625409

Gene position: 7972900-7973958 (Clockwise)

Preceding gene: 116625406

Following gene: 116625415

Centisome position: 80.0

GC content: 65.82

Gene sequence:

>1059_bases
GTGAACCTTTCCGAATTCGATTACCACCTGCCCGACGAATTGATCGCGCAGGAAGCCCTCGCCGACCGCGCCGCTTCCCG
TATGCTGGTAGTCCATCGCGAGCAGGGCCGCTGGGAAGACCGCTGCTTTCGCGACCTGCCCGAGTTTCTCCGCCCGGGCG
ATTGCCTGGTGCTCAACGATTCGCGGGTCTTTCCGGCGCGTCTCTTCGGGCACCGTTCCGGCGTCCACTCCCTCGCCGTC
GGAAAGAACAACCCCAAGCGCCACGAGTTTCTGAGCGGCGCGGTCGAGGTCTTCCTGCTGCGTGCCGTCTCTCAAGACGG
GCGTGACTGGCAGGCGCTGGTCCGGCCCGGGCGCAAGATGCGCACGGGCGAACGCATCGTGTTCGATGAAGGGCTGGAGG
CCGAGATTATCGCGCGCGGCGAATTCGGCGAGCGGACGGTGCGCTTCCTTGGCTCGGGTGATCTCTACGCCGCCTTCGAC
CGCATCGGGCACGTGCCCCTGCCTCCCTACATCAAGCGCGACGATTCACCCGCCGACCGCGAGCGGTATCAGACCGTGTT
CGCGCGCGAGAAAGGCTCTGTCGCCGCGCCCACCGCCGGACTCCACTTCACGCCTGAGGTGATCGAGCGCTGCCAGGCCG
CCGGCGCCGACGTCGCGACGGTCACGCTCCACGTCGGCCTGGGAACCTTCCAGCCGCTGCATCAGGAGGTGGTGGAAGAG
GTCAAGCTGCACACGGAGCATTACCGGATTACGGCCGATAATGCCGGAAAGATCGGGGCGGCGACGCGTGTGGTAGCGGT
CGGCACCACCAGCGTGCGCACGCTGGAGACGGCCGCCCGCGACGGTGTGCTGGAAGGCGAAACCGACATCTTCCTTTACC
CTGGAGTCCCCTTCCGCCGGACCGGCGCCATGCTCACGAATTTTCACCTGCCGCGCACCAGCCTGCTGGTGCTGGTCAGT
GCTTTCGCCGGGAAGGATCTCATGCTGGCGGCGTACCGTCACGCCGTGGAAGCCCGCTATCGTTTCTACTCCTACGGCGA
TTGCATGCTGATTGTGTGA

Upstream 100 bases:

>100_bases
CGAAAACGCCGGCAGCACCGCCGCCCCGCACAGGAGTTGTCGTCGATTCATGATCTACACGATACCCCCACTCGCCCTCT
TTCGCTACATTAAAAGCTGA

Downstream 100 bases:

>100_bases
TCGGGAGAGGGTCCGATAGGTGTGGTTCATAGGTGTCGTGAGGACGCTCCCTAACGGTCGCGGCTTCCATGAGAAGGCCC
CCCTCTGTCAAGAAAATAAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNLSEFDYHLPDELIAQEALADRAASRMLVVHREQGRWEDRCFRDLPEFLRPGDCLVLNDSRVFPARLFGHRSGVHSLAV
GKNNPKRHEFLSGAVEVFLLRAVSQDGRDWQALVRPGRKMRTGERIVFDEGLEAEIIARGEFGERTVRFLGSGDLYAAFD
RIGHVPLPPYIKRDDSPADRERYQTVFAREKGSVAAPTAGLHFTPEVIERCQAAGADVATVTLHVGLGTFQPLHQEVVEE
VKLHTEHYRITADNAGKIGAATRVVAVGTTSVRTLETAARDGVLEGETDIFLYPGVPFRRTGAMLTNFHLPRTSLLVLVS
AFAGKDLMLAAYRHAVEARYRFYSYGDCMLIV

Sequences:

>Translated_352_residues
MNLSEFDYHLPDELIAQEALADRAASRMLVVHREQGRWEDRCFRDLPEFLRPGDCLVLNDSRVFPARLFGHRSGVHSLAV
GKNNPKRHEFLSGAVEVFLLRAVSQDGRDWQALVRPGRKMRTGERIVFDEGLEAEIIARGEFGERTVRFLGSGDLYAAFD
RIGHVPLPPYIKRDDSPADRERYQTVFAREKGSVAAPTAGLHFTPEVIERCQAAGADVATVTLHVGLGTFQPLHQEVVEE
VKLHTEHYRITADNAGKIGAATRVVAVGTTSVRTLETAARDGVLEGETDIFLYPGVPFRRTGAMLTNFHLPRTSLLVLVS
AFAGKDLMLAAYRHAVEARYRFYSYGDCMLIV
>Mature_352_residues
MNLSEFDYHLPDELIAQEALADRAASRMLVVHREQGRWEDRCFRDLPEFLRPGDCLVLNDSRVFPARLFGHRSGVHSLAV
GKNNPKRHEFLSGAVEVFLLRAVSQDGRDWQALVRPGRKMRTGERIVFDEGLEAEIIARGEFGERTVRFLGSGDLYAAFD
RIGHVPLPPYIKRDDSPADRERYQTVFAREKGSVAAPTAGLHFTPEVIERCQAAGADVATVTLHVGLGTFQPLHQEVVEE
VKLHTEHYRITADNAGKIGAATRVVAVGTTSVRTLETAARDGVLEGETDIFLYPGVPFRRTGAMLTNFHLPRTSLLVLVS
AFAGKDLMLAAYRHAVEARYRFYSYGDCMLIV

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=364, Percent_Identity=40.3846153846154, Blast_Score=258, Evalue=4e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SOLUE (Q01SU0)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827565.1
- ProteinModelPortal:   Q01SU0
- SMR:   Q01SU0
- STRING:   Q01SU0
- GeneID:   4425488
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6354
- NMPDR:   fig|234267.9.peg.6039
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- PhylomeDB:   Q01SU0
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39289; Mature: 39289

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEFDYHLPDELIAQEALADRAASRMLVVHREQGRWEDRCFRDLPEFLRPGDCLVLND
CCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
SRVFPARLFGHRSGVHSLAVGKNNPKRHEFLSGAVEVFLLRAVSQDGRDWQALVRPGRKM
CCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
RTGERIVFDEGLEAEIIARGEFGERTVRFLGSGDLYAAFDRIGHVPLPPYIKRDDSPADR
CCCCEEEECCCCCEEEEECCCCCCHHHHEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
ERYQTVFAREKGSVAAPTAGLHFTPEVIERCQAAGADVATVTLHVGLGTFQPLHQEVVEE
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
VKLHTEHYRITADNAGKIGAATRVVAVGTTSVRTLETAARDGVLEGETDIFLYPGVPFRR
HHHHHHEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHH
TGAMLTNFHLPRTSLLVLVSAFAGKDLMLAAYRHAVEARYRFYSYGDCMLIV
CCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure
MNLSEFDYHLPDELIAQEALADRAASRMLVVHREQGRWEDRCFRDLPEFLRPGDCLVLND
CCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
SRVFPARLFGHRSGVHSLAVGKNNPKRHEFLSGAVEVFLLRAVSQDGRDWQALVRPGRKM
CCCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
RTGERIVFDEGLEAEIIARGEFGERTVRFLGSGDLYAAFDRIGHVPLPPYIKRDDSPADR
CCCCEEEECCCCCEEEEECCCCCCHHHHEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
ERYQTVFAREKGSVAAPTAGLHFTPEVIERCQAAGADVATVTLHVGLGTFQPLHQEVVEE
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHH
VKLHTEHYRITADNAGKIGAATRVVAVGTTSVRTLETAARDGVLEGETDIFLYPGVPFRR
HHHHHHEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHH
TGAMLTNFHLPRTSLLVLVSAFAGKDLMLAAYRHAVEARYRFYSYGDCMLIV
CCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA