Click here to switch to the map view.

The map label for this gene is truB

Identifier: 116625354

GI number: 116625354

Start: 7917578

End: 7918459

Strand: Reverse

Name: truB

Synonym: Acid_6299

Alternate gene names: 116625354

Gene position: 7918459-7917578 (Counterclockwise)

Preceding gene: 116625355

Following gene: 116625352

Centisome position: 79.46

GC content: 63.49

Gene sequence:

>882_bases
TTGGACGGAGTGATCGTAATCGACAAGCCCGAGGGCTGGACCTCGCACGACGTGGTGAACAAGGTTCGCCGCATCGCCAA
GACCAAGAAGGTCGGCCACCTCGGGACGCTGGACCCGATCGCCACGGGAGTGCTGCCGCTAGTGATCGAGCGCGCCACTC
GCCTGGCGCAGTTTTACACGCGCAGCGAGAAAATCTACGAGGGGCTGGTTCGCTTTGGCTGGTCCACGAGCAGCTACGAT
CGCGCCGGAGAACCCACCAGTGAAAAGGTCGAGGTACAGTTGACGGCGGAACAACTGGAGCATGCGCTCGAAAACTTCCG
CGGCGAGTTCCTCCAGCGGCCGCCCGCGGTTTCGGCCAAGAAAGTGGAGGGGAAACGCTCCTACGAACTCGCGCGTAAGA
ACATGGCGGTGGAACTGGAACCGGTGAAGGTCCACGTGTACGAATTGACGCTACTCGCGCTCGAAGGCAACCTGGCGCGA
TTGCGGGCGCACTGCTCGGGCGGGACTTACATGCGCTCGATCGCGCACGATCTGGGCCAGGCGTTGGGATGCGGCGCGCA
CCTGCAGGAGCTGCGCCGGCTGGCCAGCGGCGAATTCGAACTCGACCAGGCGCGTACGCTCGAGCAACTGGAATCGCTGG
CCGCCGAAGACCGGCTGCCGGATGCCATCGTGCCCGCAGGGAAGCTACTCCCCGCATTCCCCAGCGTGTTCGTGGACGAG
ATCACCGCGGCGCAAATTCGGAATGGACGCGATTTCCCGGCTTCGCCTTTTCGCTCGCAGCCCGCGTCGAAATATGTGAA
GGCCGTGACGCGCACCGGCGACCTTGTAGCGATCGGCGAGGTAGTGCTGCCCAACCTTTATCACCCGACCGTCGTGATGT
AG

Upstream 100 bases:

>100_bases
TCCTGCCGCTTCTGGACCGGCTGGATCGGGACCGCAATTTCACACTAGGCTACATCTGCACGGCGCGGCGGCGCGCCTGA
CAGAGCCATAAAGGAAACCT

Downstream 100 bases:

>100_bases
CGCAGCTATCCGGCGCGGCGCGCGGCCGTGTGCCACGCCGAATTAAGCCATTTCGCGAACGTGGTGAGGCCGGGATATTC
CTGACGTACGGATCCGATAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDGVIVIDKPEGWTSHDVVNKVRRIAKTKKVGHLGTLDPIATGVLPLVIERATRLAQFYTRSEKIYEGLVRFGWSTSSYD
RAGEPTSEKVEVQLTAEQLEHALENFRGEFLQRPPAVSAKKVEGKRSYELARKNMAVELEPVKVHVYELTLLALEGNLAR
LRAHCSGGTYMRSIAHDLGQALGCGAHLQELRRLASGEFELDQARTLEQLESLAAEDRLPDAIVPAGKLLPAFPSVFVDE
ITAAQIRNGRDFPASPFRSQPASKYVKAVTRTGDLVAIGEVVLPNLYHPTVVM

Sequences:

>Translated_293_residues
MDGVIVIDKPEGWTSHDVVNKVRRIAKTKKVGHLGTLDPIATGVLPLVIERATRLAQFYTRSEKIYEGLVRFGWSTSSYD
RAGEPTSEKVEVQLTAEQLEHALENFRGEFLQRPPAVSAKKVEGKRSYELARKNMAVELEPVKVHVYELTLLALEGNLAR
LRAHCSGGTYMRSIAHDLGQALGCGAHLQELRRLASGEFELDQARTLEQLESLAAEDRLPDAIVPAGKLLPAFPSVFVDE
ITAAQIRNGRDFPASPFRSQPASKYVKAVTRTGDLVAIGEVVLPNLYHPTVVM
>Mature_293_residues
MDGVIVIDKPEGWTSHDVVNKVRRIAKTKKVGHLGTLDPIATGVLPLVIERATRLAQFYTRSEKIYEGLVRFGWSTSSYD
RAGEPTSEKVEVQLTAEQLEHALENFRGEFLQRPPAVSAKKVEGKRSYELARKNMAVELEPVKVHVYELTLLALEGNLAR
LRAHCSGGTYMRSIAHDLGQALGCGAHLQELRRLASGEFELDQARTLEQLESLAAEDRLPDAIVPAGKLLPAFPSVFVDE
ITAAQIRNGRDFPASPFRSQPASKYVKAVTRTGDLVAIGEVVLPNLYHPTVVM

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=32.2727272727273, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4503337, Length=197, Percent_Identity=35.0253807106599, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI215599015, Length=197, Percent_Identity=35.0253807106599, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI2367200, Length=295, Percent_Identity=37.6271186440678, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=32.8205128205128, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=248, Percent_Identity=33.4677419354839, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324037, Length=136, Percent_Identity=38.2352941176471, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=31.4720812182741, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_SOLUE (Q01SZ5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827510.1
- ProteinModelPortal:   Q01SZ5
- SMR:   Q01SZ5
- STRING:   Q01SZ5
- GeneID:   4425512
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6299
- NMPDR:   fig|234267.9.peg.5983
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q01SZ5
- ProtClustDB:   CLSK2516883
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32333; Mature: 32333

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGVIVIDKPEGWTSHDVVNKVRRIAKTKKVGHLGTLDPIATGVLPLVIERATRLAQFYT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSEKIYEGLVRFGWSTSSYDRAGEPTSEKVEVQLTAEQLEHALENFRGEFLQRPPAVSAK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHH
KVEGKRSYELARKNMAVELEPVKVHVYELTLLALEGNLARLRAHCSGGTYMRSIAHDLGQ
HCCCHHHHHHHHHCCEEEEEEEEEEEEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHH
ALGCGAHLQELRRLASGEFELDQARTLEQLESLAAEDRLPDAIVPAGKLLPAFPSVFVDE
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
ITAAQIRNGRDFPASPFRSQPASKYVKAVTRTGDLVAIGEVVLPNLYHPTVVM
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDGVIVIDKPEGWTSHDVVNKVRRIAKTKKVGHLGTLDPIATGVLPLVIERATRLAQFYT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSEKIYEGLVRFGWSTSSYDRAGEPTSEKVEVQLTAEQLEHALENFRGEFLQRPPAVSAK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHH
KVEGKRSYELARKNMAVELEPVKVHVYELTLLALEGNLARLRAHCSGGTYMRSIAHDLGQ
HCCCHHHHHHHHHCCEEEEEEEEEEEEEEEEEEECCCHHHHHHHCCCCHHHHHHHHHHHH
ALGCGAHLQELRRLASGEFELDQARTLEQLESLAAEDRLPDAIVPAGKLLPAFPSVFVDE
HHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
ITAAQIRNGRDFPASPFRSQPASKYVKAVTRTGDLVAIGEVVLPNLYHPTVVM
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA