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The map label for this gene is azoB [H]

Identifier: 116625353

GI number: 116625353

Start: 7916691

End: 7917572

Strand: Direct

Name: azoB [H]

Synonym: Acid_6298

Alternate gene names: 116625353

Gene position: 7916691-7917572 (Clockwise)

Preceding gene: 116625351

Following gene: 116625358

Centisome position: 79.44

GC content: 60.66

Gene sequence:

>882_bases
ATGCCAGCCAACAAGAACAAAAACAAAGTGATCCTTGTCACCGGGGCGACCGGCCATCAGGGCGGAGCTGTCGTTCGTCA
CTTGCGCGAGAAGGGCTTTACGGTACGCGCACTTACGCGCGATCCGGATCAGCCGAAGGCGCGTGCAATCACCGGCCAGG
GCGTAGAGGTCGTCCGCGGAGATATGGATGACAAAGCCGTACTTACGCGCGCGCTGGACGAGGCGAACGGCGTTTTTTCC
GTACAAAATTCGCACGAGGCCGGAATCGAGGGTGAAATTCGCCAGGGCCTCGGCGTTGCCGACGCGGCCAAACGCTCCCG
CATTTCGCATTTCATTTACAGCTCCGTGGCGAGCGCGGACCAGAAGACCGGTATCCCCCACTTCGACAGTAAATTCCGCA
TCGAGGAGCACATACGCGGGACGGGTATGAACTTCACGATCGTGCGGCCGGTCTTCTTCATGGAGAACTGGCTCGGCATG
CGCGAGATGATCGAGAACGGCGCGCTGAATCTTCCTCTGGATCCCGCGACCCGGTTGCAGATGATCGCGGTGGACGATAT
CGGTGGTGTAGTGGCCGCCGCCTTCGAACGGCCGGGCAAATGGCAGGGCCGCACCTTCGAAGTCGCTGGGGACGAGATGT
CGATGACCGAGCTTACCCAGGCCTTCACGCTCGTCACAGGGCATGAAGTTCGCTACGTTCAGACGCCGTGGGATGAGTTC
GAGCAGCGGGCCGGAAAAGAAATTACGACGATGTACCGCTGGTTCCAGGCGGCCGGCTATCACGTGGATATCGGATCCGT
ACGTCAGGAATATCCCGGCCTCACCACGTTCGCGAAATGGCTTAATTCGGCGTGGCACACGGCCGCGCGCCGCGCCGGAT
AG

Upstream 100 bases:

>100_bases
CGAAGCGAGTGCAGTTGTTTTCATTGTCTGTATCATACTGCGCGCAGGCGCGAATCCGGCCTGATGGATACCTTGGCACG
GCGGATGCTCGTCTTCATAC

Downstream 100 bases:

>100_bases
CTGCGCTACATCACGACGGTCGGGTGATAAAGGTTGGGCAGCACTACCTCGCCGATCGCTACAAGGTCGCCGGTGCGCGT
CACGGCCTTCACATATTTCG

Product: NmrA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPANKNKNKVILVTGATGHQGGAVVRHLREKGFTVRALTRDPDQPKARAITGQGVEVVRGDMDDKAVLTRALDEANGVFS
VQNSHEAGIEGEIRQGLGVADAAKRSRISHFIYSSVASADQKTGIPHFDSKFRIEEHIRGTGMNFTIVRPVFFMENWLGM
REMIENGALNLPLDPATRLQMIAVDDIGGVVAAAFERPGKWQGRTFEVAGDEMSMTELTQAFTLVTGHEVRYVQTPWDEF
EQRAGKEITTMYRWFQAAGYHVDIGSVRQEYPGLTTFAKWLNSAWHTAARRAG

Sequences:

>Translated_293_residues
MPANKNKNKVILVTGATGHQGGAVVRHLREKGFTVRALTRDPDQPKARAITGQGVEVVRGDMDDKAVLTRALDEANGVFS
VQNSHEAGIEGEIRQGLGVADAAKRSRISHFIYSSVASADQKTGIPHFDSKFRIEEHIRGTGMNFTIVRPVFFMENWLGM
REMIENGALNLPLDPATRLQMIAVDDIGGVVAAAFERPGKWQGRTFEVAGDEMSMTELTQAFTLVTGHEVRYVQTPWDEF
EQRAGKEITTMYRWFQAAGYHVDIGSVRQEYPGLTTFAKWLNSAWHTAARRAG
>Mature_292_residues
PANKNKNKVILVTGATGHQGGAVVRHLREKGFTVRALTRDPDQPKARAITGQGVEVVRGDMDDKAVLTRALDEANGVFSV
QNSHEAGIEGEIRQGLGVADAAKRSRISHFIYSSVASADQKTGIPHFDSKFRIEEHIRGTGMNFTIVRPVFFMENWLGMR
EMIENGALNLPLDPATRLQMIAVDDIGGVVAAAFERPGKWQGRTFEVAGDEMSMTELTQAFTLVTGHEVRYVQTPWDEFE
QRAGKEITTMYRWFQAAGYHVDIGSVRQEYPGLTTFAKWLNSAWHTAARRAG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190720, Length=287, Percent_Identity=29.9651567944251, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 32488; Mature: 32357

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANKNKNKVILVTGATGHQGGAVVRHLREKGFTVRALTRDPDQPKARAITGQGVEVVRG
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHEEECCCCCEEEEC
DMDDKAVLTRALDEANGVFSVQNSHEAGIEGEIRQGLGVADAAKRSRISHFIYSSVASAD
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QKTGIPHFDSKFRIEEHIRGTGMNFTIVRPVFFMENWLGMREMIENGALNLPLDPATRLQ
HHCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MIAVDDIGGVVAAAFERPGKWQGRTFEVAGDEMSMTELTQAFTLVTGHEVRYVQTPWDEF
EEEECCCCHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHEECCEEEEEECCHHHH
EQRAGKEITTMYRWFQAAGYHVDIGSVRQEYPGLTTFAKWLNSAWHTAARRAG
HHHHCHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PANKNKNKVILVTGATGHQGGAVVRHLREKGFTVRALTRDPDQPKARAITGQGVEVVRG
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHEEECCCCCEEEEC
DMDDKAVLTRALDEANGVFSVQNSHEAGIEGEIRQGLGVADAAKRSRISHFIYSSVASAD
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QKTGIPHFDSKFRIEEHIRGTGMNFTIVRPVFFMENWLGMREMIENGALNLPLDPATRLQ
HHCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MIAVDDIGGVVAAAFERPGKWQGRTFEVAGDEMSMTELTQAFTLVTGHEVRYVQTPWDEF
EEEECCCCHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHEECCEEEEEECCHHHH
EQRAGKEITTMYRWFQAAGYHVDIGSVRQEYPGLTTFAKWLNSAWHTAARRAG
HHHHCHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]