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The map label for this gene is purA [H]

Identifier: 116625330

GI number: 116625330

Start: 7879453

End: 7881060

Strand: Direct

Name: purA [H]

Synonym: Acid_6275

Alternate gene names: 116625330

Gene position: 7879453-7881060 (Clockwise)

Preceding gene: 116625329

Following gene: 116625331

Centisome position: 79.07

GC content: 55.29

Gene sequence:

>1608_bases
ATGTTCAGCGAAATCGTTCTCCTATCAGGTCCGGTAGCGTCAGGGAAAAGCACCTTGGCCTATGCGTTGGTGCGGAGTTA
CGGTTTTGATTGGTGTAAGACGCGTGAACTGATCCAAAGACTGAAGAGCACGAAGGGTGTACGCAGCGCCCTCCAGACCG
CAGGGGACGGACTCGATCGACGCACGAAGGGTGAATGGATAGCCACGGCTGTTGCAGAGCGCTCTCGAACGATCCCAGAG
AATGCCCGCATTGTCGTGGATGCGGTTCGAGCCAAGTCCCAGATCGACGCAGTCCGTAGGGCTTTCGGCCCGCTCGTGGT
CCACATCCACCTCACAGCGCCAGAAGAAGATCTGCGCCAGCGGTATGAGAACGGCCGATCCCCTTCTATCGAGGAATTTA
GCAGTTATGCCGAAGTTCGCCAGAATAAGACTGAGCGGTTCGTAGAACGCCTCGCTGACGTTGCCGACATAGTTATCAAT
ACCGGGCATAGCACCGAGGAAGATGTTCTTGTGCGCGTTGCGAGTCGCTTAGGACTGTACGGCAGATCGGTCGATCGGCT
AGTTGATGTTCTCGTTGGCGGGCAGTACGGAAGTGAAGGTAAAGGGCATGTGGCATCCTATCTGGCCCCAGAATATGATT
TTTTAGTTCGCGCGGGCGGTCCGAACGCCGGCCATACGGTCTACGAGGAGCCGAACGCATATGCCCACCACCAACTTCCG
TCCGGCACCAGATTTTCTAATTCGAAACTCGTCATAGCGCCTGGCGCGGTCTTGGCCGTCGAGAAGTTGCTGAAGGAGAT
CGCAGATTGCCAGGTAGATAATGAACGACTTACCATCGATCCCCAAGCCATGATTATCGAGCCCGGTGACATCAGTTTTG
AAGAAAAGGGGCTCAAGAATAGCATCGGCAGCACCGCCCAGGGCGTTGGCTCTGCGAGTAGCCGGAAGATACTTCGAACG
GCAGCGCGACCGAAGGTAAAGCTGGCTAAAGACGTAGTCCAGTTGCGACCGTTTGTGAAGGAGACGTTGGGCATACTCGA
GCGGGCGTTTGCGCGCGGTCAGCGAGTTTTCCTCGAGGGAACACAAGGGACCGGACTTAGTATTCACCACGGCTTCTACC
CATACGTGACCTCCCGCGAAACTTCGGTGAGCGGTTGCTTGGCGGAAGCTGGGATAGCGCCTACCCGCGTGCGACGGACG
ATCATTGCGGTTCGGAGCTATCCGATTCGCGTTGAGAGCCCGATGGACGCGACCTCTGGGCCAATGAAGCGAGAGCTAGA
TTGGGCAACAATTTCCTTTCGATCCGGCATTCCGGTCGAGGAACTGGAGGAAAAAGAAAAAACGACAACTACGAAGAAGC
GGCGGAGAGTGGCCGAATTCGATTGGTCCCTTCTCAGGAAATCGGCGTCCCTTAACGGTCCCACTGATGTAGCGTTGACT
TTCGTAGACTATATCAATGTTCATAATCGAAAGGCTCGCCGCTTCGAGCAACTCACGTCGATCACGGTGCAACTTATCGA
GGAAATCGAGCGGGTTGCCGCCGCACCAGTTTCACTTATAACGACCAGATTCGACTATCGCAGTATCATAGATCGGCGGG
CATGGTAA

Upstream 100 bases:

>100_bases
GGGTTCCCGTCTTTGTTTACGATTTACCTCAGACCACGCCAGTTCAACCGGCGCAGATGGCATTGGAGTCATCACTCGAA
TAGAGCCGAGGCGACACGAC

Downstream 100 bases:

>100_bases
TGGGCATATCAATAGACAACCTGGCAGCTACATATCCGCGCTTGTATCACATCACCAGCGCTGGAACTTGGCCAAGCATC
CTGCGGCACGGGCTTCTGAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MFSEIVLLSGPVASGKSTLAYALVRSYGFDWCKTRELIQRLKSTKGVRSALQTAGDGLDRRTKGEWIATAVAERSRTIPE
NARIVVDAVRAKSQIDAVRRAFGPLVVHIHLTAPEEDLRQRYENGRSPSIEEFSSYAEVRQNKTERFVERLADVADIVIN
TGHSTEEDVLVRVASRLGLYGRSVDRLVDVLVGGQYGSEGKGHVASYLAPEYDFLVRAGGPNAGHTVYEEPNAYAHHQLP
SGTRFSNSKLVIAPGAVLAVEKLLKEIADCQVDNERLTIDPQAMIIEPGDISFEEKGLKNSIGSTAQGVGSASSRKILRT
AARPKVKLAKDVVQLRPFVKETLGILERAFARGQRVFLEGTQGTGLSIHHGFYPYVTSRETSVSGCLAEAGIAPTRVRRT
IIAVRSYPIRVESPMDATSGPMKRELDWATISFRSGIPVEELEEKEKTTTTKKRRRVAEFDWSLLRKSASLNGPTDVALT
FVDYINVHNRKARRFEQLTSITVQLIEEIERVAAAPVSLITTRFDYRSIIDRRAW

Sequences:

>Translated_535_residues
MFSEIVLLSGPVASGKSTLAYALVRSYGFDWCKTRELIQRLKSTKGVRSALQTAGDGLDRRTKGEWIATAVAERSRTIPE
NARIVVDAVRAKSQIDAVRRAFGPLVVHIHLTAPEEDLRQRYENGRSPSIEEFSSYAEVRQNKTERFVERLADVADIVIN
TGHSTEEDVLVRVASRLGLYGRSVDRLVDVLVGGQYGSEGKGHVASYLAPEYDFLVRAGGPNAGHTVYEEPNAYAHHQLP
SGTRFSNSKLVIAPGAVLAVEKLLKEIADCQVDNERLTIDPQAMIIEPGDISFEEKGLKNSIGSTAQGVGSASSRKILRT
AARPKVKLAKDVVQLRPFVKETLGILERAFARGQRVFLEGTQGTGLSIHHGFYPYVTSRETSVSGCLAEAGIAPTRVRRT
IIAVRSYPIRVESPMDATSGPMKRELDWATISFRSGIPVEELEEKEKTTTTKKRRRVAEFDWSLLRKSASLNGPTDVALT
FVDYINVHNRKARRFEQLTSITVQLIEEIERVAAAPVSLITTRFDYRSIIDRRAW
>Mature_535_residues
MFSEIVLLSGPVASGKSTLAYALVRSYGFDWCKTRELIQRLKSTKGVRSALQTAGDGLDRRTKGEWIATAVAERSRTIPE
NARIVVDAVRAKSQIDAVRRAFGPLVVHIHLTAPEEDLRQRYENGRSPSIEEFSSYAEVRQNKTERFVERLADVADIVIN
TGHSTEEDVLVRVASRLGLYGRSVDRLVDVLVGGQYGSEGKGHVASYLAPEYDFLVRAGGPNAGHTVYEEPNAYAHHQLP
SGTRFSNSKLVIAPGAVLAVEKLLKEIADCQVDNERLTIDPQAMIIEPGDISFEEKGLKNSIGSTAQGVGSASSRKILRT
AARPKVKLAKDVVQLRPFVKETLGILERAFARGQRVFLEGTQGTGLSIHHGFYPYVTSRETSVSGCLAEAGIAPTRVRRT
IIAVRSYPIRVESPMDATSGPMKRELDWATISFRSGIPVEELEEKEKTTTTKKRRRVAEFDWSLLRKSASLNGPTDVALT
FVDYINVHNRKARRFEQLTSITVQLIEEIERVAAAPVSLITTRFDYRSIIDRRAW

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790620, Length=345, Percent_Identity=28.4057971014493, Blast_Score=109, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25146553, Length=373, Percent_Identity=29.4906166219839, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25146555, Length=355, Percent_Identity=29.8591549295775, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6324109, Length=364, Percent_Identity=27.1978021978022, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356033, Length=346, Percent_Identity=29.7687861271676, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 59320; Mature: 59320

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS01259 BH3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSEIVLLSGPVASGKSTLAYALVRSYGFDWCKTRELIQRLKSTKGVRSALQTAGDGLDR
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RTKGEWIATAVAERSRTIPENARIVVDAVRAKSQIDAVRRAFGPLVVHIHLTAPEEDLRQ
CCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHH
RYENGRSPSIEEFSSYAEVRQNKTERFVERLADVADIVINTGHSTEEDVLVRVASRLGLY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GRSVDRLVDVLVGGQYGSEGKGHVASYLAPEYDFLVRAGGPNAGHTVYEEPNAYAHHQLP
CCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SGTRFSNSKLVIAPGAVLAVEKLLKEIADCQVDNERLTIDPQAMIIEPGDISFEEKGLKN
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCEECCCEEEECCCEEEECCCCCCHHHHCHHH
SIGSTAQGVGSASSRKILRTAARPKVKLAKDVVQLRPFVKETLGILERAFARGQRVFLEG
HCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TQGTGLSIHHGFYPYVTSRETSVSGCLAEAGIAPTRVRRTIIAVRSYPIRVESPMDATSG
CCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
PMKRELDWATISFRSGIPVEELEEKEKTTTTKKRRRVAEFDWSLLRKSASLNGPTDVALT
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FVDYINVHNRKARRFEQLTSITVQLIEEIERVAAAPVSLITTRFDYRSIIDRRAW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSEIVLLSGPVASGKSTLAYALVRSYGFDWCKTRELIQRLKSTKGVRSALQTAGDGLDR
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RTKGEWIATAVAERSRTIPENARIVVDAVRAKSQIDAVRRAFGPLVVHIHLTAPEEDLRQ
CCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHH
RYENGRSPSIEEFSSYAEVRQNKTERFVERLADVADIVINTGHSTEEDVLVRVASRLGLY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GRSVDRLVDVLVGGQYGSEGKGHVASYLAPEYDFLVRAGGPNAGHTVYEEPNAYAHHQLP
CCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SGTRFSNSKLVIAPGAVLAVEKLLKEIADCQVDNERLTIDPQAMIIEPGDISFEEKGLKN
CCCCCCCCEEEEECCHHHHHHHHHHHHHCCEECCCEEEECCCEEEECCCCCCHHHHCHHH
SIGSTAQGVGSASSRKILRTAARPKVKLAKDVVQLRPFVKETLGILERAFARGQRVFLEG
HCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TQGTGLSIHHGFYPYVTSRETSVSGCLAEAGIAPTRVRRTIIAVRSYPIRVESPMDATSG
CCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
PMKRELDWATISFRSGIPVEELEEKEKTTTTKKRRRVAEFDWSLLRKSASLNGPTDVALT
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
FVDYINVHNRKARRFEQLTSITVQLIEEIERVAAAPVSLITTRFDYRSIIDRRAW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]