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The map label for this gene is gatA [H]

Identifier: 116625172

GI number: 116625172

Start: 7647950

End: 7649356

Strand: Reverse

Name: gatA [H]

Synonym: Acid_6116

Alternate gene names: 116625172

Gene position: 7649356-7647950 (Counterclockwise)

Preceding gene: 116625173

Following gene: 116625171

Centisome position: 76.76

GC content: 66.81

Gene sequence:

>1407_bases
ATGACCCCAGCCCTAGCCTCATTCCCCGATGCCGCAGGCCTGGCGCGCATCATTCGCTCGGGCGGCCTCAGCGCAGTGGA
AGCAGTAACGATGACTCTGGGTAAGATCGACCGGTATGACCGGTCAGTGAATTGTTTCACCACTGTGCTGCGCGAATCCG
CGCTGTCGGATGCGGCGGAGATCGACCGGCGCCTTGCTGCCGGCGAAGATCCGGGACCGCTGTGCGGCGTGCCATTCGCG
GTCAAGAACCTGTTCGACATCGCGGGATTGACGACGCTGGCGGGATCGAAGATTCACGCCGAATGTGCGCCCGCGGCGCA
CGACGCCACGGTAGTGGAGCGCTTGAAGTCGGCGGGAGCGATTGCCGTCGGCGCGCTCAACATGGACGAGTACGCCTACG
GGTTCACGACCGAGAACTCGCACTACGGTCCGACGCGCAACCCGCACGACCTAGAGCGCGTGGCTGGCGGATCATCGGGC
GGATCGGCGGCGGCGGTGGCGGCGGGGCTGGTGCCGCTGACGCTGGGCTCGGACACCAATGGCTCGATTCGCGTGCCGGC
GGCGTTCTGCGGGGTCTTCGGACTGAAGCCGACGTACGGCCGGATCTCGCGCGCGGGCGCGTTTCTATTTTGCGAGAGCT
TCGATCATGTGGGTCCGTTCGCGCGATCGGTCGGAGATCTGGCGCTGGCGTTCGACACTCTGCACGGGCCCGACGCGCGC
GATCCGGTGTGCAGCGCACGGCAGGCCGAGCCAGTGCTGCCACAACTGGAGCGCGGCATCGACGGATTGCGCATTGCGGT
CGCCGGGGGGTACTTCGCGCGGCGGGGAATGCCGGAAGCCTTCGCGCCAGTAACGACCGCGGCGCGCGCGCTGGGCGTGA
TGCGCACGGTTGAGGTTCCGGACTCGGATGTGGCGCGTGCCGCGGCGTACGTGATTACGGCGTGCGAGGGCAGCCGTATG
CACTTTCAAGACCTGAAGACGCGGCCGCAGGATTTCGATCCGTTGGTGGTGGACCGCTTCTTGGCGGGCGCGCTTCTGCC
GGCGACGTGGTACCAGCAGGCGCAGCGTTTCCGAACGGTGTTTCGGGCCAAGGTGCGGGAGTTGTTTCAGAACGTCGACA
TCCTGCTGGCGCCCGCGACGCCTTGCCCCGCGATTCGCATCGGACAGGCCACAATCACGCTGGACGGAGTCGAGTTGCCG
TCGCGGCCGAGTGTCGGTCTGTTTACGCAGCCGCTGTCTTCGATCGGGCTGCCGATTGTGGTGGCGCCGGTATTCGGGCC
GGGAATGTTGCCGGCAGGCCTGCAAATAGTCGGTGCCCCTTACAATGAGTCCGCGGTGCTGCGGGTGGCGCGCTACCTGG
AGAAGATCGGTGTGGCGGCGGCGCCGGTGGCGGAGGGCTTTGCATGA

Upstream 100 bases:

>100_bases
AAGCGGTGCGGATCAACCTGGAGCGGGTCTCAGCGATTTTGCGGCCGCTACTGGAATTCCCGATTGACGAGAGTGTGGAA
TCCGCGCCGGTGTTCCTGCC

Downstream 100 bases:

>100_bases
GCGGGCTTTCGCGGCGCCAATTCGTGGGCGGAGCCGCGGCGGCCTGCGCCAGGGCCGGAATGCAGGATCCGCTGGTTCTC
GGCTTTCTTTATAACGGTCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTPALASFPDAAGLARIIRSGGLSAVEAVTMTLGKIDRYDRSVNCFTTVLRESALSDAAEIDRRLAAGEDPGPLCGVPFA
VKNLFDIAGLTTLAGSKIHAECAPAAHDATVVERLKSAGAIAVGALNMDEYAYGFTTENSHYGPTRNPHDLERVAGGSSG
GSAAAVAAGLVPLTLGSDTNGSIRVPAAFCGVFGLKPTYGRISRAGAFLFCESFDHVGPFARSVGDLALAFDTLHGPDAR
DPVCSARQAEPVLPQLERGIDGLRIAVAGGYFARRGMPEAFAPVTTAARALGVMRTVEVPDSDVARAAAYVITACEGSRM
HFQDLKTRPQDFDPLVVDRFLAGALLPATWYQQAQRFRTVFRAKVRELFQNVDILLAPATPCPAIRIGQATITLDGVELP
SRPSVGLFTQPLSSIGLPIVVAPVFGPGMLPAGLQIVGAPYNESAVLRVARYLEKIGVAAAPVAEGFA

Sequences:

>Translated_468_residues
MTPALASFPDAAGLARIIRSGGLSAVEAVTMTLGKIDRYDRSVNCFTTVLRESALSDAAEIDRRLAAGEDPGPLCGVPFA
VKNLFDIAGLTTLAGSKIHAECAPAAHDATVVERLKSAGAIAVGALNMDEYAYGFTTENSHYGPTRNPHDLERVAGGSSG
GSAAAVAAGLVPLTLGSDTNGSIRVPAAFCGVFGLKPTYGRISRAGAFLFCESFDHVGPFARSVGDLALAFDTLHGPDAR
DPVCSARQAEPVLPQLERGIDGLRIAVAGGYFARRGMPEAFAPVTTAARALGVMRTVEVPDSDVARAAAYVITACEGSRM
HFQDLKTRPQDFDPLVVDRFLAGALLPATWYQQAQRFRTVFRAKVRELFQNVDILLAPATPCPAIRIGQATITLDGVELP
SRPSVGLFTQPLSSIGLPIVVAPVFGPGMLPAGLQIVGAPYNESAVLRVARYLEKIGVAAAPVAEGFA
>Mature_467_residues
TPALASFPDAAGLARIIRSGGLSAVEAVTMTLGKIDRYDRSVNCFTTVLRESALSDAAEIDRRLAAGEDPGPLCGVPFAV
KNLFDIAGLTTLAGSKIHAECAPAAHDATVVERLKSAGAIAVGALNMDEYAYGFTTENSHYGPTRNPHDLERVAGGSSGG
SAAAVAAGLVPLTLGSDTNGSIRVPAAFCGVFGLKPTYGRISRAGAFLFCESFDHVGPFARSVGDLALAFDTLHGPDARD
PVCSARQAEPVLPQLERGIDGLRIAVAGGYFARRGMPEAFAPVTTAARALGVMRTVEVPDSDVARAAAYVITACEGSRMH
FQDLKTRPQDFDPLVVDRFLAGALLPATWYQQAQRFRTVFRAKVRELFQNVDILLAPATPCPAIRIGQATITLDGVELPS
RPSVGLFTQPLSSIGLPIVVAPVFGPGMLPAGLQIVGAPYNESAVLRVARYLEKIGVAAAPVAEGFA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=28.2, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=34.453781512605, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI166795287, Length=183, Percent_Identity=36.6120218579235, Blast_Score=84, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=27.6255707762557, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=196, Percent_Identity=35.7142857142857, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17537465, Length=249, Percent_Identity=32.1285140562249, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=518, Percent_Identity=23.3590733590734, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=193, Percent_Identity=33.6787564766839, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=193, Percent_Identity=33.6787564766839, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=199, Percent_Identity=33.1658291457286, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=193, Percent_Identity=30.5699481865285, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=31.6455696202532, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=395, Percent_Identity=27.3417721518987, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=196, Percent_Identity=31.1224489795918, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648113, Length=463, Percent_Identity=32.6133909287257, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24644968, Length=493, Percent_Identity=27.1805273833671, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24648435, Length=484, Percent_Identity=26.8595041322314, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648437, Length=484, Percent_Identity=26.8595041322314, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45550774, Length=484, Percent_Identity=26.8595041322314, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=27.2912423625255, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=35.6846473029046, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652985, Length=241, Percent_Identity=33.195020746888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=241, Percent_Identity=33.195020746888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=241, Percent_Identity=33.195020746888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=241, Percent_Identity=33.195020746888, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648441, Length=419, Percent_Identity=26.9689737470167, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648439, Length=419, Percent_Identity=26.9689737470167, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49001; Mature: 48869

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPALASFPDAAGLARIIRSGGLSAVEAVTMTLGKIDRYDRSVNCFTTVLRESALSDAAE
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IDRRLAAGEDPGPLCGVPFAVKNLFDIAGLTTLAGSKIHAECAPAAHDATVVERLKSAGA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCC
IAVGALNMDEYAYGFTTENSHYGPTRNPHDLERVAGGSSGGSAAAVAAGLVPLTLGSDTN
EEEEEECCCHHEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCC
GSIRVPAAFCGVFGLKPTYGRISRAGAFLFCESFDHVGPFARSVGDLALAFDTLHGPDAR
CCEECCHHHHHHHCCCCCHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCC
DPVCSARQAEPVLPQLERGIDGLRIAVAGGYFARRGMPEAFAPVTTAARALGVMRTVEVP
CCHHCCCCCCCCHHHHHCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHEECCC
DSDVARAAAYVITACEGSRMHFQDLKTRPQDFDPLVVDRFLAGALLPATWYQQAQRFRTV
CHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAKVRELFQNVDILLAPATPCPAIRIGQATITLDGVELPSRPSVGLFTQPLSSIGLPIV
HHHHHHHHHCCCCEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCHHHCCHHHCCCCEE
VAPVFGPGMLPAGLQIVGAPYNESAVLRVARYLEKIGVAAAPVAEGFA
EECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure 
TPALASFPDAAGLARIIRSGGLSAVEAVTMTLGKIDRYDRSVNCFTTVLRESALSDAAE
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IDRRLAAGEDPGPLCGVPFAVKNLFDIAGLTTLAGSKIHAECAPAAHDATVVERLKSAGA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCC
IAVGALNMDEYAYGFTTENSHYGPTRNPHDLERVAGGSSGGSAAAVAAGLVPLTLGSDTN
EEEEEECCCHHEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCC
GSIRVPAAFCGVFGLKPTYGRISRAGAFLFCESFDHVGPFARSVGDLALAFDTLHGPDAR
CCEECCHHHHHHHCCCCCHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCC
DPVCSARQAEPVLPQLERGIDGLRIAVAGGYFARRGMPEAFAPVTTAARALGVMRTVEVP
CCHHCCCCCCCCHHHHHCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHEECCC
DSDVARAAAYVITACEGSRMHFQDLKTRPQDFDPLVVDRFLAGALLPATWYQQAQRFRTV
CHHHHHHHHHHEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAKVRELFQNVDILLAPATPCPAIRIGQATITLDGVELPSRPSVGLFTQPLSSIGLPIV
HHHHHHHHHCCCCEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCHHHCCHHHCCCCEE
VAPVFGPGMLPAGLQIVGAPYNESAVLRVARYLEKIGVAAAPVAEGFA
EECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA